形觉剥夺性弱视幼猫视皮质17区生长相关蛋白-43的表达

发布时间：2018-01-03 04:11

  本文关键词：形觉剥夺性弱视幼猫视皮质17区生长相关蛋白-43的表达　出处：《眼科新进展》2015年03期 　论文类型：期刊论文

  更多相关文章： 形觉剥夺 弱视 生长相关蛋白- 左旋多巴

【摘要】：目的观察形觉剥夺性弱视动物模型应用左旋多巴(levodopa,LD)治疗后视皮质17区生长相关蛋白-43(growth associated protein-43,GAP-43)的表达,探讨LD对形觉剥夺性弱视视觉功能恢复作用的可能机制。方法50只4周龄幼猫,随机分为正常组、剥夺组、对照组、低剂量LD组及高剂量LD组共5组,每组10只。通过单眼眼睑缝合制备形觉剥夺的弱视模型,观察不同干预条件下各组图形视觉诱发电位(pattern visual evoked potential,PVEP)的P100波的峰时及振幅的变化。应用免疫组织化学法测定各组视皮层17区GAP-43的差异。结果8周龄时,剥夺组、对照组、低剂量LD组、高剂量LD组剥夺眼P100波振幅比对侧眼及同周龄正常组幅值降低,潜时延长(均为P0.05);12周龄时,低剂量LD组剥夺眼P100波振幅增高,与对侧眼及同周龄正常组相比差异均无统计学意义(均为P0.05),而P100波潜时则未达到正常(P0.05);高剂量LD组剥夺眼P100波振幅增高、潜时缩短,与对侧眼及同周龄正常组相比差异均无统计学意义(均为P0.05)。8周龄时剥夺组视皮质17区Ⅱ/Ⅲ、Ⅳ、Ⅴ/Ⅵ层GAP-43免疫阳性细胞密度均低于正常组,差异均有统计学意义(均为P0.05);12周龄时高剂量LD组各层GAP-43免疫阳性细胞密度与正常组基本相等,差异无统计学意义(P0.05);对照组、低剂量LD组和正常组相比差异均有统计学意义(均为P0.05)。结论GAP-43在形觉剥夺性弱视形成过程中起一定的作用,LD对形觉剥夺性弱视视功能改善的机制是通过GAP-43表达的改变而实现的。
[Abstract]:Objective To observe the effect of deprivation amblyopia animal model application of levodopa (levodopa, LD) in visual cortex area 17 growth associated protein -43 (growth associated protein-43, GAP-43) the expression of LD on the perception of the possible mechanism of amblyopia visual function. Methods: 50 4 week old kittens were randomly divided into normal. Group, sleep deprivation group, control group, low dose LD group and high dose LD group 5 group, 10 rats in each group. By monocular lid suture preparing to form deprivation amblyopia model, observe the different interference conditions of each pattern visual evoked potential (pattern visual evoked potential, PVEP) changes of the P100 wave peak and the difference of amplitude. Determination of visual cortex area 17 GAP-43 immunohistochemical method. Results 8 weeks of age, deprivation group, control group, low dose LD group, high-dose LD group deprived eyes P100 wave amplitude contralateral eye and age-matched normal amplitude reduction 浣，

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