18-β甘草次酸对变应性鼻炎大鼠鼻黏膜上皮细胞内质网的影响

发布时间：2018-01-11 11:14

本文关键词：18-β甘草次酸对变应性鼻炎大鼠鼻黏膜上皮细胞内质网的影响　出处：《中国中西医结合杂志》2015年05期 　论文类型：期刊论文

【摘要】：目的探讨18-β甘草次酸(glycyrrhetinic acid,GA)对变应性鼻炎(allergic rhinitis,AR)模型大鼠鼻黏膜上皮细胞内质网变化的影响。方法 96只Wistar大鼠随机分为空白组、AR模型组、氯雷他定组、甘草次酸组,每组24只,腹腔注射卵清蛋白致敏建立大鼠AR模型,评定动物形态学评分。造模后给予甘草次酸21.6 mg/kg灌胃,干预后第2、4、6、10周取鼻黏膜组织送电镜检查。结果空白组叠加评分(2.10±0.45)分,氯雷他定组为(5.10±0.56)分,AR模型组为(5.10±0.56)分,甘草次酸组为(5.20±0.78)分,各组分别与空白组比较,差异有统计学意义(P0.01)。透射电镜见AR模型组内质网较空白组增多,明显囊样扩张、大量空泡形成、脱颗粒,胞质内可见大量的游离核糖体。在变应原持续作用下,AR模型组鼻黏膜上皮细胞内质网的上述变化呈进行性加重。而甘草次酸干预组内质网扩张、空泡化、脱颗粒均较模型组缓解,并随观察周期延长逐渐接近空白对照组。结论 18-β甘草次酸可改善变应性鼻炎模型大鼠鼻黏膜上皮细胞内质网的扩张、空泡化及脱颗粒。
[Abstract]:Objective to investigate the effect of 18- 尾 glycyrrhetinic acid (GA) on allergic rhinitis allergic rhinitis. Methods 96 Wistar rats were randomly divided into three groups: control group (n = 24), loratadine group (n = 24) and glycyrrhetinic acid group (n = 24). The AR model was established by intraperitoneal injection of ovalbumin to sensitize the rats, and the animal morphological score was evaluated. After the model was established, glycyrrhetinic acid was given orally for 21.6 mg/kg. Results the superposition score was 2.10 卤0.45 in blank group and 5.10 卤0.56 in loratadine group. The scores of AR model group and glycyrrhetinic acid group were 5.10 卤0.56 and 5.20 卤0.78, respectively. Transmission electron microscope showed that the endoplasmic reticulum of AR model group was more than that of the blank group, the cystoid dilatation, a large number of cavitation formation, degranulation. A large number of free ribosomes could be seen in cytoplasm. The changes of endoplasmic reticulum (ER) of nasal epithelial cells in AR model group were gradually aggravated under the continuous action of allergen, while endoplasmic reticulum dilated and vacuolated in glycyrrhetinic acid intervention group. Compared with the model group, the degranulation was relieved, and gradually close to the blank control group with the prolonged observation period. Conclusion 18- 尾 glycyrrhetinic acid can improve the expansion of endoplasmic reticulum of nasal mucosa epithelial cells in rats with allergic rhinitis. Vacuolation and degranulation.
【作者单位】：兰州大学第一医院耳鼻咽喉头颈外科;甘肃中医学院附属医院耳鼻咽喉头颈科;
【基金】：国家自然科学基金资助项目(N0.81160449) 甘肃省卫生厅行业科技计划项目(N0.GSWST2011-06) 甘肃省中医药科学技术研究课题(N0.GZK-2011-22)
【分类号】：R765.21
【正文快照】： 变应性鼻炎是由Ig E介导的鼻黏膜的变态反应性疾病。发病机制属于1型超敏反应,与细胞因子、细胞间黏附分子及部分神经肽密切相关,其过程涉及T、B等淋巴细胞的激活及白介素、肿瘤坏死因子等细胞因子的合成及相关炎症介质的释放[1]。与线粒体一样内质网被认为在细胞生命活动中起

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