白血病抑制因子（LIF）促进兔眼LASIK术后角膜神经修复的实验研究

发布时间：2018-01-16 22:04

本文关键词：白血病抑制因子（LIF）促进兔眼LASIK术后角膜神经修复的实验研究　出处：《重庆医科大学》2011年硕士论文　论文类型：学位论文

【摘要】：目的:探讨白血病抑制因子(Leukemia inhibitory factor,LIF)对兔眼准分子激光原位角膜磨镶术(laser in situ keratomileusis,LASIK)后角膜神经修复的促进作用,并观察评估白血病抑制因子局部使用的安全性。方法:40只健康新西兰大白兔,其中5只兔作为空白组,不作任何处理,用于观察正常兔眼的角膜神经形态;其余35只兔(70只眼)均接受近视性LASIK手术,术后左眼给予自制的1.0μg/mlLIF滴眼液,4次/天,作为治疗组;右眼给予平衡盐溶液(balanced salt solution,BSS),4次/天,作为对照组。分别于术后第1天、第1周、第2周、第1个月、第3个月及第6个月进行干眼参数检查,并各取5只兔处死后摘取双侧角膜行氯化金染色,光镜下观察角膜神经修复情况,并进行两组比较。余下5只兔于术后第6个月处死后摘下双侧角膜,每只角膜平均分成两半,一半用于制作角膜病理切片,光镜下观察LIF滴眼液使用6个月后对角膜各层细胞结构的影响,另一半行透射电镜观察LIF滴眼液使用6个月后对角膜各层超微结构的影响。结果:1.LASIK术后除蒂部以外,角膜瓣切削区上皮下及浅基质层神经均消失,术后第7天角膜瓣边缘可见少量再生神经纤维,第6个月时上皮下、浅基质层神经数量和形态接近正常,周边部角膜神经网密度与术前无明显差异,而中央密度仍低于术前。 2. LASIK术后各时间点除第6个月时,治疗组神经末梢数量均高于对照组,有统计学差异(P0.05)。 3.术后两组干眼参数比较,第2周、1月、3月治疗组均高于对照组,差异有统计学意义,其余各期比较无统计学差异。 4.LASIK术后局部使用LIF滴眼液治疗的6个月内,并未发现角膜新生血管形成、上皮剥脱、晶体混浊等不良反应,光镜和电镜下观察兔眼角膜各层结构均无明显变化。结论:1. LASIK手术造成兔眼大部分角膜神经的丢失,除蒂部以外,角膜瓣切削区上皮下及浅基质层神经均消失。 2.LIF能有效促进LASIK术后兔眼角膜神经的修复,从而改善术后干眼现象,是一种安全有效的药物。
[Abstract]:Objective: to investigate the leukemia inhibitory factor Leukemia inhibitory factor. The effect of lif on corneal nerve repair after laser in situ keratomileusis in situ keratomileusis in rabbits was studied. The safety of local use of leukemia suppressor was evaluated. Methods of 40 healthy New Zealand white rabbits, 5 rabbits were used as blank group, without any treatment, to observe the corneal nerve morphology of normal rabbits. The other 35 rabbits (70 eyes) were treated with myopic LASIK operation. The left eye was treated with self-made 1.0 渭 g / ml LIF eye drops 4 times a day as the treatment group. The right eye was treated with balanced salt solution four times a day as the control group, respectively on the first day, the first week and the second week after operation. The dry eye parameters were examined in the first month, the third month and the sixth month, and 5 rabbits were killed respectively. The corneas were harvested and stained with gold chloride. The nerve repair of the cornea was observed under light microscope. The remaining 5 rabbits were killed at the 6th month after operation. Each cornea was divided into two halves, half of which was used to make pathological sections of the cornea. The effects of LIF eye drops on the cell structure of each layer of cornea were observed under light microscope, and the effects of LIF eye drops on the ultrastructure of each layer of cornea were observed by transmission electron microscope (TEM). Results 1. After LASIK, the subcutaneous and superficial stromal layer nerves in the corneal flap disappeared except for the pedicle. A small amount of regenerated nerve fibers were observed at the edge of the corneal flap on the 7th day after operation, and at the 6th month. The number and morphology of the superficial stromal nerve were close to normal, the density of the peripheral corneal nerve network was not significantly different from that of the preoperative, but the central density was still lower than that of the preoperation. 2. At 6 months after LASIK, the number of nerve endings in the treatment group was higher than that in the control group (P 0.05). 3. The dry eye parameters of the two groups were higher than that of the control group in the 2nd week, January and March, the difference was statistically significant, but there was no statistical difference in the other stages. 4. Within 6 months after LASIK local use of LIF eye drops, no adverse reactions such as corneal neovascularization, epithelial exfoliation and lens opacity were found. There was no obvious change in corneal structure under light microscope and electron microscope. Conclusion 1. LASIK procedure caused the loss of most corneal nerves in rabbit eyes, and the subcutaneous and superficial stromal nerves in the corneal flap region disappeared except for the pedicle. 2. LIF can effectively promote the corneal nerve repair after LASIK and improve the dry eye phenomenon. It is a safe and effective drug.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R779.63

【参考文献】