低氧预适应对缺血再灌注损伤大鼠视网膜低氧诱导因子-1α和血红素氧合酶-1表达的影响

发布时间：2018-01-22 23:10

本文关键词： 视网膜缺血再灌注损伤低氧预适应低氧诱导因子-α 血红素氧合酶-　出处：《眼科新进展》2015年04期 　论文类型：期刊论文

【摘要】：目的观察低氧预适应(hypoxic preconditioning,HPC)后视网膜缺血再灌注损伤(retinal ischemia-reperfusion injury,RIRI)大鼠视网膜低氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)、血红素氧合酶-1(heme oxygenase-1,HO-1)的动态变化。方法取65只Wistar成年大鼠随机分为正常对照组5只,RIRI组30只,HPC+RIRI组30只(HPC后采用高眼压法制成RIRI模型),后两组于RIRI后6 h、12 h、24 h、36 h、3 d、7 d各处死5只鼠。采用尼氏染色观察HPC对各组大鼠视网膜的影响,免疫组织化学法动态观察HPC对RIRI大鼠视网膜HIF-1α、HO-1表达的影响。结果尼氏染色显示正常对照组大鼠视网膜结构清晰,各层细胞排列整齐紧密;RIRI组大鼠视网膜各层高度水肿,随着时间延长,神经节细胞数量逐渐减少,分布较紊乱、稀疏;HPC+RIRI组与RIRI组比较,各层细胞水肿明显减轻,细胞排列比较规整,神经节细胞数量减少降低,分布较整齐。正常对照组大鼠视网膜组织HIF-1α、HO-1微量表达。RIRI组HIF-1α表达升高,峰值在RIRI后12 h,阳性细胞数为(120.64±5.67)个·mm-2;RIRI后6 h起HPC+RIRI组HIF-1α表达较RIRI组升高,RIRI后12 h达高峰且阳性细胞数为(158.54±4.77)个·mm-2,24 h起逐渐下降,RIRI后7 d时HPC+RIRI组HIF-1α表达仍高于RIRI组;RIRI后RIRI组HO-1表达升高,峰值在RIRI后24 h且阳性细胞数为(129.54±4.58)个·mm-2;RIRI后6 h起HPC+RIRI组HO-1表达较RIRI组升高,RIRI后12 h达高峰且阳性细胞数为(157.56±4.67)个·mm-2,RIRI后24 h仍高表达且阳性细胞数为(150.78±4.97)个·mm-2,此后逐渐下降,RIRI后7 d,HPC+RIRI组仍明显高于RIRI组。与RIRI组比较,HPC+RIRI组RIRI后各时间点HIF-1α、HO-1阳性细胞数均增多,差异均有统计学意义(均为P0.05)。结论 HPC可促进RIRI大鼠视网膜HIF-1α、HO-1蛋白的表达,减轻视网膜损伤,具有视网膜保护作用。
[Abstract]:Objective to observe hypoxic preconditioning in hypoxic preconditioning. Retinal ischemia-reperfusion injury after retinal ischemia-reperfusion injury. Rat retinal hypoxic inducible factor-1 伪 (hypoxia inducible factor-1 伪). Methods 65 adult Wistar rats were randomly divided into normal control group (n = 5). In RIRI group, 30 rats in RIRI group were treated with high intraocular pressure (IOP) to form RIRI model, and the latter two groups were treated with RIRI at 6 h, 12 h, 24 h and 36 h, respectively. The effects of HPC on the retina of rats in each group were observed by Nissl staining. The retinal HIF-1 伪 of RIRI rats was observed dynamically by immunohistochemical method. Results the retinal structure of normal control group was clear and the cells of each layer were arranged neatly and tightly. In RIRI group, the number of ganglion cells decreased gradually, and the distribution of ganglion cells was disordered and sparse. In HPC RIRI group, compared with RIRI group, the edema of cells and the number of ganglion cells in each layer were obviously reduced, the cell arrangement was more regular and the number of ganglion cells was decreased. The expression of HIF-1 伪 -HO-1 in retina of normal control group was increased. The expression of HIF-1 伪 in RIRI group was increased, and the peak value was 12 hours after RIRI. The number of positive cells was 120. 64 卤5. 67) 路mm-2; From 6 h after RIRI, the expression of HIF-1 伪 in HPC RIRI group was higher than that in RIRI group. The number of positive cells was 158.54 卤4.77 路m ~ (-2) 路m ~ (-2) at 12 h after RIRI and gradually decreased after 24 h. At 7 days after RIRI, the expression of HIF-1 伪 in HPC RIRI group was still higher than that in RIRI group. The expression of HO-1 in RIRI group increased after RIRI, the peak value was 24 h after RIRI and the number of positive cells was 129.54 卤4.58 路mm-2; The expression of HO-1 in HPC RIRI group was higher than that in RIRI group from 6 hours after RIRI. The number of positive cells was 157.56 卤4.67 路mm-2 at 12 h after RIRI. The number of positive cells was 150.78 卤4.97 路m ~ (-2) 路m ~ (-2) at 24 h after RIRI, and then decreased gradually 7 days after RIRI. Compared with RIRI group, the number of HIF-1 伪 -pHO-1 positive cells in HPC RIRI group was significantly higher than that in RIRI group at all time points after RIRI. Conclusion HPC can promote the expression of HIF-1 伪 -HO-1 protein in retina of RIRI rats and alleviate retinal injury. It has protective effect of retina.
【作者单位】：潍坊医学院眼科教研室;潍坊医学院附属医院眼科;潍坊医学院医学影像学系分子影像学研究中心;
【基金】：国家自然科学基金项目(编号:81000268) 山东省自然科学基金项目(编号:ZR2013HL067) 山东省科技发展计划项目(编号:2010GW2202):~~
【分类号】：R774.1
【正文快照】： 随着糖尿病视网膜病变、视网膜动静脉血管阻塞等常见的视网膜缺血性疾病发病率的升高,对于视网膜缺血再灌注损伤(retinal ischemia-reperfusioninjury,RIRI)的预防和治疗也成为眼科学者的研究热点,然而血-视网膜屏障的存在限制了许多药物的临床应用。低氧预适应(hypoxic preco

【参考文献】