脂多糖诱导炎症对大鼠氧诱导视网膜病变的影响

发布时间：2018-02-03 08:29

本文关键词： 炎症氧诱导视网膜病变视网膜新生血管早产儿视网膜病变　出处：《眼科新进展》2015年04期 　论文类型：期刊论文

【摘要】：目的观察不同剂量脂多糖(lipopolysaccharide,LPS)诱导炎症对大鼠氧诱导视网膜病变(oxygen-induced retinopathy,OIR)的影响,以探讨炎症在早产儿视网膜病变(retinopathy of prematurity,ROP)中的作用。方法将新生SD大鼠随机分组,实验组依据LPS注射剂量的不同分为LPS-50、LPS-100和LPS-500组,并分别设立正常对照组(N组)和氧诱导对照组(OIR组)。N组幼鼠喂养于空气中,实验组和OIR组幼鼠饲养于氧气体积分数为80%/21%(24 h交替一次)的氧箱中至出生后14 d(P14),随后移至空气中继续饲养。实验组幼鼠行OIR处理过程中,于P7行LPS腹腔注射,LPS-50、LPS-100和LPS-500组注射剂量分别为50μg·kg-1、100μg·kg-1和500μg·kg-1。检测各组幼鼠在P7(注射前)、P8、P11和P14等不同时间点的体质量变化;并分别于P14和P18制作全视网膜铺片,通过GS-isolectin B4染色观察各组视网膜血管化及病理性新生血管情况。结果在各检测时间点,各实验组和OIR组幼鼠的体质量均低于N组(P0.05)。P7时,各实验组和OIR组体质量无差别(P0.05);至P8、P11和P14时,LPS-500组幼鼠体质量明显低于OIR组和其他实验组(P0.05)。P14时,N组幼鼠的浅层视网膜血管已覆盖整个视网膜;而OIR组和实验组幼鼠的视网膜均存在无血管区,且LPS-500组无血管区面积最大(27.32%±3.58%,P0.01)。P18时,N组视网膜血管网层次结构清晰;OIR组和实验组幼鼠的视网膜均出现病理性新生血管,且LPS-500组新生血管累及视网膜的范围最广(累及钟点数为6.83±1.72,P0.01)。结论 LPS诱导炎症可加重大鼠OIR,且在一定范围内呈剂量依赖性,提示炎症可能参与ROP病理过程。
[Abstract]:Objective to observe lipopolysaccharide in different doses. Effects of LPS-induced inflammation on oxygen-induced retinopathy (OIR) in rats with oxygen-induced retinopathy. To investigate the role of inflammation in retinopathy of prematurity retinopathy (ROP). Methods newborn SD rats were randomly divided into two groups. The experimental group was divided into LPS-50 LPS-100 and LPS-500 group according to the different injection dose of LPS. Normal control group (n group) and oxygen-induced control group (OIR group) were set up respectively. The young rats of group N were fed in the air. The young rats in the experimental group and the OIR group were fed in an oxygen box with oxygen volume fraction of 80 / 21 and 24 hours alternated to 14 days after birth (P14). The young rats in the experimental group were treated with OIR and LPS was injected intraperitoneally with LPS-50 in P7. The injection doses of LPS-100 and LPS-500 were 50 渭 g 路kg ~ (-1) kg-1 and 500 渭 g 路kg ~ (-1) respectively. The changes of body mass at different time points, such as P8, P11 and P14; The retinal vascularization and pathological neovascularization were observed by GS-isolectin B4 staining. The body mass of young rats in each experimental group and OIR group was lower than that in group N (P0.05A. P7). There was no difference in body weight between the experimental group and OIR group (P0.05). The body mass of the young rats in LPS-500 group was significantly lower than that in OIR group and other experimental groups at the time of P11 and P14. The superficial retinal vessels of the young rats in group N had covered the whole retina. However, there was no vascular area in the retina of OIR group and experimental group, and the area of no vessel area was 27.32% 卤3.58% in LPS-500 group. The level of retinal vascular network in group N was clear. Pathological neovascularization appeared in the retina of OIR group and experimental group, and the range of retinal involvement in LPS-500 group was the most extensive (the number of hours involved was 6.83 卤1.72). Conclusion LPS induced inflammation can aggravate OIRs in a dose-dependent manner, suggesting that inflammation may be involved in the pathological process of ROP.
【作者单位】：第四军医大学西京医院眼科全军眼科研究所;
【基金】：国家自然科学基金资助(编号:81271014;81470655) 德国洪堡基金会(Alexander von Humboldt Foundation)仪器设备捐赠基金资助(to YS Wang,V-8151/02085)~~
【分类号】：R774.1
【正文快照】： 视网膜血管的正常发育对视功能的形成至关重要,当未成熟的视网膜暴露于某些危险因素时,可出现正常的视网膜血管化过程受阻,继而形成病理性新生血管,导致早产儿视网膜病变(retinopathy of prematurity,ROP),造成视力损害,严重者甚至失明。作为儿童的首位致盲性疾病,ROP发生的

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