京尼平角膜交联的长期安全性初步研究

发布时间：2018-02-10 16:38

本文关键词： 京尼平角膜胶原交联共焦显微镜安全性　出处：《眼科新进展》2015年01期 　论文类型：期刊论文

【摘要】：目的探讨生物交联剂京尼平在角膜交联中的长期安全性。方法选取健康新西兰白兔22只,分为实验组(10只)、阳性对照组(10只)以及正常对照组(2只),均取右眼为实验眼,其中实验组应用2 g·L-1京尼平在室温下频繁滴眼40 min,阳性对照组应用体积分数0.1%戊二醛在室温下频繁滴眼40 min,正常对照组不做任何处理。术后观察动物外眼及眼前节情况,分别在滴眼后1 d、7 d、14 d、30 d、90 d每组处死2只动物(正常对照组在滴眼后7 d处死2只动物),取右眼眼球,先后进行角膜共焦显微镜检查和组织病理学观察。结果实验组对角膜、结膜的刺激作用轻于阳性对照组。各组角膜层次结构清晰,初步观察实验组及阳性对照组相同深度的角膜基质细胞数与正常对照组比较未见明显差异。滴眼后1 d、7 d、14 d、30 d、90 d,阳性对照组角膜内皮细胞计数分别为(2437±55)mm-2、(2478±81)mm-2、(2272±69)mm-2、(2574±118)mm-2、(2481±93)mm-2,均小于实验组(2713±89)mm-2、(2613±100)mm-2、(2620±98)mm-2、(2749±92)mm-2、(2678±89)mm-2和正常对照组(2645±90)mm-2,差异均有统计学意义(P=0.000、0.001),实验组与正常对照组差异无统计学意义(P=0.530)。各组兔眼角膜结构排列整齐,细胞形态正常,实验组和阳性对照组基质层胶原纤维排列较正常对照组更紧密。结论 2 g·L-1京尼平溶液作为一种天然的生物交联剂,具有刺激性低、对角膜基质及内皮细胞无明显损伤等优点,初步认为是一种安全的角膜交联剂。
[Abstract]:Objective to investigate the long-term safety of genipin in corneal crosslinking. Methods 22 healthy New Zealand rabbits were divided into experimental group (n = 10) and control group (n = 10) and normal control group (n = 2). The experimental group was treated with 2 g 路L -1 geniapine for 40 minutes, the positive group was given 0.1% glutaraldehyde for 40 minutes at room temperature, and the normal control group was not treated. The external eye and anterior segment were observed after operation. Two animals were killed in each group at 1 day, 7 days, 14 days, 30 days and 90 days after eye dripping. In the normal control group, 2 animals were killed at 7 days after dropping, the right eye was taken, corneal confocal microscopy and histopathological observation were performed successively. Results the cornea of the experimental group were examined by confocal microscopy and histopathological observation. The stimulating effect of conjunctiva was lighter than that of positive control group. The number of corneal stromal cells of the same depth in the experimental group and the positive control group was not significantly different from that in the normal control group. The number of corneal endothelial cells in the positive control group was 2437 卤55mm-2, 2478 卤81mm-2, 2574 卤118mm-2mm-2, 2481 卤93mm-2, respectively, less than that in the control group, which was less than that in the control group. The experimental group (2713 卤89mm-2) and the control group (2613 卤100mm-2) were 2620 卤98mm-2mm-2 and 2749 卤92mm-2mm-2, respectively. The difference was statistically significant between the control group (2645 卤90mm-2) and the control group (2645 卤90mm-2). There was no significant difference between the experimental group and the normal control group. There was no significant difference between the experimental group and the normal control group. The corneal structure of the rabbits was arranged neatly and neatly, and the corneal structure of the experimental group was not significantly different from that of the normal control group. The matrix layer collagen fibers of the experimental group and the positive control group were arranged more closely than that of the normal control group. Conclusion as a natural biological crosslinking agent, 2 g 路L -1 geniapine solution has low irritation. It has no obvious damage to corneal stroma and endothelial cells, so it is considered to be a safe cross-linking agent for cornea.
【作者单位】：南京军区南京总医院眼科;北京大学第一医院眼科;
【基金】：国家自然科学基金(编号:11372011) 北京市自然科学基金(编号:7142159) 中国科学院力学研究所非线性力学国家重点实验室基金~~
【分类号】：R772.2

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