Notch基因在变应性鼻炎小鼠模型中的表达及其意义

发布时间：2018-02-13 03:14

本文关键词： Notch基因鼻炎变应性模型动物　出处：《临床耳鼻咽喉头颈外科杂志》2014年20期 　论文类型：期刊论文

【摘要】：目的:探讨Notch信号通路相关基因在变应性鼻炎模型小鼠鼻黏膜及外周血单个核细胞(PBMC)中的表达及意义。方法:7~8周龄BALB/c小鼠16只,随机分成对照组和模型组。以卵清蛋白作为过敏原,建立变应性鼻炎小鼠模型,分析比较小鼠过敏症状评分,采用苏木精-伊红染色分析比较鼻黏膜病理变化及炎性细胞的浸润程度,采用酶联免疫吸附法检测并分析小鼠外周血血清中IgE的表达水平。荧光定量聚合酶链反应和流式细胞术从不同水平检测模型组小鼠鼻黏膜及PBMC中Notch信号通路相关受体(Notch1~Notch4)的表达量,并与对照组比较。结果:成功建立了变应性鼻炎BALB/c小鼠模型。模型组小鼠鼻黏膜中Notch1、Notch3、Notch4mRNA的表达明显高于对照组(P0.01),而Notch2mRNA的表达却低于对照组(P0.05);与此一致,模型组小鼠PBMC中Notch1、Notch3、Notch4的蛋白表达也明显高于对照组(P0.01),而Notch2的蛋白却低于对照组(P0.05)。结论:Notch基因的表达在变应性鼻炎模型小鼠发病过程中有显著改变,可能参与变应性鼻炎的发生发展,为深入研究变应性鼻炎的发病机制提供了思路。
[Abstract]:Objective: to investigate the expression and significance of Notch signaling pathway related genes in nasal mucosa and peripheral blood mononuclear cells (PBMC) of allergic rhinitis mice. The mouse model of allergic rhinitis was established with ovalbumin as allergen, and the allergic symptom score of mice was analyzed and compared. The pathological changes of nasal mucosa and the degree of infiltration of inflammatory cells were analyzed by hematoxylin and eosin staining. The expression of IgE in peripheral blood of mice was detected and analyzed by enzyme-linked immunosorbent assay (Elisa). Fluorescence quantitative Polymerase chain reaction (FQ-PCR) and flow Cytometry (FCM) were used to detect the Notch signaling pathway in the nasal mucosa and PBMC of the model group from different levels. The expression of Notch1 (Notch4), Results: the BALB/c mouse model of allergic rhinitis was successfully established. The expression of Notch1ntch3ntch4 mRNA in the nasal mucosa of mice in the model group was significantly higher than that in the control group (P 0.01), but the expression of Notch2mRNA was lower than that in the control group (P 0.05). The protein expression of Notch1, Notch3, Notch4 in PBMC of the model group was also significantly higher than that of the control group, while the protein of Notch2 was lower than that of the control group. Conclusion the expression of the 1 / Notch gene in allergic rhinitis model mice was significantly changed during the pathogenesis of allergic rhinitis. It may be involved in the occurrence and development of allergic rhinitis, which provides a way to further study the pathogenesis of allergic rhinitis.
【作者单位】：西安交通大学医学院第二附属医院耳鼻咽喉头颈外科病院;西安交通大学医院耳鼻咽喉科;
【基金】：西安交通大学交叉项目(No:08143039) 国家自然科学基金(No:81271087)
【分类号】：R765.21

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