耳蜗核前腹核丛细胞的功能修饰先于听觉的建立

发布时间：2018-02-12 23:29

本文关键词： 耳蜗核前腹侧核丛细胞发育电生理　出处：《南方医科大学学报》2014年07期 　论文类型：期刊论文

【摘要】：目的通过全细胞膜片钳技术研究新生SD乳鼠耳蜗核前腹核(AVCN)中丛细胞在发育过程中的电生理特性变化。方法运用全细胞膜片钳记录出生后5~21 d的SD乳鼠耳蜗核前腹核中丛细胞的动作电位(AP)反应及微小兴奋性突触后电流(mEPSC),再通过相关数据采集及分析软件分析动作电位半峰宽和mEPSC的10%~90%上升时间(10%~90%Risetime)及时间常数,并对此做进一步统计分析处理,进而比较不同年龄段新生SD乳鼠的耳蜗核前腹核中丛细胞的电生理反应特性。结果在丛细胞动作电位半峰宽的统计分析中发现,丛细胞动作电位的时间精确性随着出生后年龄的增加而增加,在听觉建立(出生后12 d)附近变化停止。丛细胞mEPSC的时间精确性也随着出生后年龄的增加而增加,在听觉建立附近变化停止。结论耳蜗核前腹核中丛细胞功能的修饰先于听觉的建立。
[Abstract]:Objective to study the electrophysiological characteristics of plexus cells in the anterior ventral nucleus of the cochlear nucleus (AVCNN) of newborn SD rats by whole-cell patch clamp technique. Methods whole cell patch clamp was used to record the precochlear nucleus precochlear nucleus of neonatal SD rats at 5 to 21 days after birth. The action potential (APP) response and the minimal excitatory postsynaptic current (mEPSCN) of the plexus cells in the ventral nucleus were analyzed. The half peak width of the action potential and the 1090% rise time of mEPSC and the time constant were analyzed by the software of relevant data acquisition and analysis. The electrophysiological responses of the plexus cells in the anterior ventral nucleus of the cochlear nucleus of newborn SD rats of different ages were compared. Results in the statistical analysis of the half peak width of the action potential of the plexus cells, the electrophysiological response of the plexus cells in the anterior ventral nucleus of the cochlear nucleus was compared. The temporal accuracy of the action potential of plexus cells increased with the increase of postnatal age, and stopped in the vicinity of auditory establishment (12 days after birth). The temporal accuracy of mEPSC of plexus cells also increased with the increase of postnatal age. Conclusion the functional modification of the plexus cells in the anterior ventral nucleus of the cochlear nucleus is prior to the establishment of the auditory system.
【作者单位】：南方医科大学基础医学院生理学教研室;
【基金】：国家自然科学基金(31171059)~~
【分类号】：R764

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