白细胞介素4及受体基因多态性与维吾尔族变应性鼻炎的关联研究

发布时间：2018-02-14 04:56

本文关键词： 受体白介素4 变应性鼻炎多态性基因型　出处：《新疆医科大学》2010年硕士论文　论文类型：学位论文

【摘要】：目的:变应性鼻炎(allergic rhinitis,AR)具有复杂的遗传背景,是遗传因素和环境因素相互作用的共同结果,对该病染色体上易感基因的探究仍然是目前的研究热点和努力方向。本研究通过分析白细胞介素4(IL-4)及受体(IL-4R)基因多态性与维吾尔族(维族)人群AR的关联,为探寻AR易感基因和疾病防治提供相关信息。方法:采用人群为基础病例对照研究设计,以新疆喀什地区现况调查发现的109例维族AR病人和393例健康对照者为研究对象,用聚合酶链反应-限制性片段长度多态性法(PCR-RFLP)检测IL-4 rs2243283C/G和IL-4R rs6498011A/G位点基因多态性,比较它们在病例组与对照组间基因型和等位基因的频率分布,并通过问卷调查分析AR相关影响因素。结果:(1)IL-4R基因rs6498011A/G位点在病例组和对照组间基因型分布频率有统计学差异( x 2=23.841,P0.001),等位基因型分布频率也有统计学差异( x 2=7.113,P=0.008),多因素分析结果同样显示IL-4R基因型是AR的影响因素,携带IL-4R基因rs6498011位点AA基因型和AG基因型者发生AR的危险分别为携带GG基因型者的7.330倍、5.316倍。(2)IL-4基因rs2243283C/G位点在两组间基因型及等位基因分布均无明显不同(P0.05)。(3)吸烟为新疆喀什地区维族AR的影响因素,吸烟者发生AR的危险性是不吸烟者的2.073倍。结论: IL-4R基因rs6498011A/G位点基因多态性与维族AR密切相关,可能是影响维族AR的重要易感侯选基因;IL-4基因rs2243283C/G位点与维族AR无相关性。
[Abstract]:Objective: allergic rhinitis ARs has a complex genetic background and is a common result of the interaction between genetic and environmental factors. In this study, the polymorphism of interleukin-4 (IL-4) and receptor IL-4R (IL-4R) gene and AR in Uygur nationality (Uygur) population were analyzed, and the relationship between the polymorphisms of IL-4 gene and AR in Uygur population was analyzed by analyzing the relationship between the polymorphism of IL-4 gene and AR in Uygur population. Methods: a population-based case-control study was conducted in 109 Uygur patients with AR and 393 healthy controls in Kashi, Xinjiang. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the polymorphism of IL-4 rs2243283C/G and IL-4R rs6498011A/G loci, and to compare the frequency distribution of genotypes and alleles between case group and control group. Results the frequency of genotype distribution of rs6498011A/G locus of IL-4R gene was significantly different between case group and control group (x2m23. 841, P0.001), and the frequency of allelic genotype was also significantly different (x 2, 7. 113, P < 0. 008, P < 0. 008). The results of factor analysis also showed that IL-4R genotype was the influencing factor of AR. The risk of AR in patients with rs6498011 and AG genotype of IL-4R gene was 7.330-fold and 5.316 times as high as that of those with GG genotype, respectively. There was no significant difference in genotype and allele distribution between the two groups. The influencing factors of Uygur AR in Xinjiang Kashi area, Conclusion: the polymorphism of rs6498011A/G locus of IL-4R gene is closely related to AR in Uygur nationality, and it may be that the rs2243283C/G locus of IL-4 gene, an important susceptible candidate gene, has no correlation with AR in Uygur nationality.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R765.21

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