小牛血去蛋白提取物与重组人表皮生长因子促进兔角膜上皮损伤愈合和血管新生

发布时间：2018-02-14 20:35

本文关键词： 角膜损伤愈合新生血管生长因子　出处：《眼科新进展》2014年05期 　论文类型：期刊论文

【摘要】：目的探讨小牛血去蛋白提取物与重组人表皮生长因子(recombinant human epithelial growth factor,rhEGF)对兔角膜上皮损伤修复及血管新生的作用。方法建立兔角膜上皮损伤及新生血管模型,随机分为三组(每组5只5眼):小牛血去蛋白提取物组(速高捷组)、rhEGF组和生理盐水组(NS组)。术后1 d开始分别滴速高捷、rhEGF和生理盐水,每天4次,观察角膜上皮损伤修复及新生血管面积变化。结果术后1 d,rhEGF组、速高捷组与NS组角膜上皮愈合率分别为(51.83±8.87)%、(39.35±6.92)%、(43.32±5.42)%,三组间差异有统计学意义(F=6.475,P=0.012),rhEGF组角膜上皮愈合率高于速高捷组(P=0.004)和NS组(P=0.041)。术后2 d、3 d,三组间角膜上皮愈合率差异均无统计学意义(均为P0.05)。术后18 d,速高捷组角膜新生血管面积(58.27±5.42)mm2小于rhEGF组(79.98±8.63)mm2和NS组(77.16±12.59)mm2,差异均有统计学意义(P=0.040、0.008)。术后8 d、12 d、15 d和21 d,三组间角膜新生血管面积差异均无统计学意义(均为P0.05)。结论 rhEGF早期(2 d内)较速高捷促进兔角膜上皮损伤修复作用明显,rhEGF和速高捷促进角膜新生血管作用不明显。
[Abstract]:Objective to investigate the effects of calf blood deproteinization extract and recombinant human epithelial growth factor-rhEGF on corneal epithelial injury and angiogenesis in rabbits. Methods Rabbit corneal epithelial injury and neovascularization models were established. The rats were randomly divided into three groups (5 eyes in each group): calf blood deproteinizing extract group (RHG group) and NS group (NS group). After 1 day of operation, rhEGF and normal saline were dripped respectively, 4 times a day. The corneal epithelial injury and neovascularization were observed. Results the rhEGF group was treated with rhEGF on the 1st day after operation. The healing rate of corneal epithelium in the two groups was 51.83 卤8.87 and 39.35 卤6.92 and 43.32 卤5.42, respectively. The difference among the three groups was statistically significant. The rate of corneal epithelial healing in the three groups was significantly higher than that in the high speed group (P 0.004) and the NS group (P 0.041). There was no statistical difference in the rate of corneal epithelial healing between the three groups at 2 days and 3 days after operation. The corneal neovascularization area was 58.27 卤5.42 mm ~ 2 in the high speed injection group and 77.16 卤12.59 mm ~ 2 in the rhEGF group and NS group, respectively. There was no significant difference in corneal neovascularization area between the three groups on the 8th day, 12 days after operation, 15 days and 21 days after the operation (P 0.05), and there was no significant difference in the area of corneal neovascularization between the three groups (P < 0.05). The corneal neovascularization area in the three groups was not significantly different from that in the control group (P < 0.05), but there was no significant difference in the corneal neovascularization area between the three groups. Conclusion the effect of rhEGF on corneal epithelium injury and repair was significantly higher than that of rhEGF within 2 days. The effect of rhEGF on corneal neovascularization was not obvious.
【作者单位】：南方医科大学珠江医院眼科;中山大学中山眼科中心国家重点实验室;
【基金】：国家自然科学基金资助(编号:30973246);国家自然科学基金面上项目(编号:81371067) 广东省广州市海珠区科技计划项目(编号:2010-Y-26)~~
【分类号】：R772.2

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