酒精法去上皮对角膜上皮紧密连接影响的研究

发布时间：2018-02-22 04:29

本文关键词： 准分子激光角膜切削术酒精大鼠角膜上皮紧密连接　出处：《安徽医科大学》2011年硕士论文　论文类型：学位论文

【摘要】：目的:探讨屈光手术中酒精法去上皮对大鼠角膜上皮紧密连接的影响。方法:60只SD大鼠随机分为酒精组和机械组,各30只。在各组内再将大鼠随机六组(A、B、C、D、E、F),每组5只,每只大鼠随机选择一只眼作为手术眼,另一只眼作为对照眼。分别用来观察24 h大鼠角膜上皮缺损直径、检测24、48、72、96、120 h角膜上皮claudin-1、ZO-1蛋白的表达情况。心脏注药法处死动物,取眼球,用OCT冰冻包埋,制作冰冻切片,HE染色,免疫荧光法检测角膜上皮紧密连接蛋白表达光密度,进行统计学比较。结果: 1.去上皮时间:酒精组和机械组去上皮时间的差异有统计学意义(P0.05)。 2.角膜上皮中央缺损直径:24 h手术显微镜下观察60只大鼠的手术眼,中央区角膜上皮均有缺损。裂隙灯照相分析酒精组和机械组A组大鼠术眼角膜上皮缺损直径的大小,酒精组和机械组角膜上皮缺损直径差异无统计学意义。手术显微镜下观察48 h实验大鼠手术眼角膜上皮光滑,均未见缺损。 3.组织病理学改变:HE染色结果显示两组48 h移行的角膜上皮完全覆盖损伤的部位,但仅2～3层上皮细胞,细胞形态异常,变大、扁平延长。96 h角膜上皮基本恢复正常形态。 4. ZO-1蛋白表达差异:ZO-1主要在角膜上皮表层细胞表达,24、48 h酒精组和机械组角膜上皮ZO-1蛋白的表达差异有统计学意义(P0.05)。72、96、120 h ZO-1蛋白的表达在酒精组和机械组差异无统计学意义。 5. claudin-1蛋白表达差异:claudin-1主要在角膜上皮翼状细胞和基底细胞层表达,24、48 h酒精组和机械组角膜上皮claudin-1蛋白的表达差异有统计学意义(P0.05)。72、96、120 h claudin-1蛋白的表达在酒精组和机械组差异无统计学意义。结论:屈光手术手术中酒精法去上皮较机械法去上皮速度快,二者角膜上皮再生时间未见明显区别。但酒精法去上皮较机械法影响角膜上皮紧密连接的修复,说明20%酒精去上皮影响角膜上皮的修复。
[Abstract]:Objective: To investigate the effect of alcohol removal on the close connection of corneal epithelium in rats during refractive surgery.
Methods: 60 SD rats were randomly divided into alcohol group and mechanical group, 30 rats each. The rats in groups randomly six groups (A, B, C, D, E, F), 5 rats in each group, each rat were randomly selected as one eye surgery eye, the other eye as the control eyes were used to observe the corneal epithelial defect. 24 h rats to detect 24,48,72,96120 h in diameter, corneal epithelial claudin-1, expression of ZO-1 protein. The heart injection were animal, eyeballs, frozen OCT embedding, the production of frozen sections, HE staining, immunofluorescence detection of corneal epithelial tight junction protein expression in the light density. The results were statistically analyzed.
Result:
1. the time to go to the epithelium: there was a significant difference in the time between the alcohol group and the mechanical group (P0.05).
The diameter of 2. central corneal epithelial defect: Observation of 60 rats 24 h eye surgery under surgical microscope, central corneal epithelium defect. Slit lamp diameter and mechanical analysis in alcohol group group A group rat corneal epithelial defect size, alcohol group and mechanical corneal epithelial defect diameter was no significant difference in operation. Under the microscope 48 h rat corneal epithelial surgery is smooth, no defect.
3. histopathological changes: HE staining showed that two h 48.96 migrated corneal epithelium completely covered the site of injury, but only 2~3 layers of epithelial cells, abnormal cell morphology, larger, flat, prolonged.96 h corneal epithelium was basically restored to normal form.
4., the difference of ZO-1 protein expression: ZO-1 was mainly expressed in the epithelial cells of corneal epithelium. The expression of ZO-1 protein in corneal epithelium of 24,48 h alcohol group and mechanical group was statistically significant (P0.05). The expression of.72,96120 h ZO-1 protein in alcohol group and mechanical group was not statistically significant.
5. claudin-1 expression: claudin-1 mainly expressed in the epithelium of cells and basal cell layer, there was significant difference in the expression of 24,48 h in alcohol group and mechanical corneal epithelial claudin-1 protein (P0.05) expression of.72,96120 h claudin-1 protein had no significant difference in the alcohol group and mechanical group.
Conclusion: alcohol refractive surgery operation. Compared with the mechanical method to epithelial epithelial fast, two corneal epithelial regeneration time had no significant difference. But the alcohol method to epithelium mechanical effects on corneal epithelial tight junction repair, 20% alcohol to repair the corneal epithelium epithelium.

【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R779.63

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