FOXP3、T淋巴细胞亚群在鼻息肉中的表达及其临床意义

发布时间：2018-02-25 04:18

本文关键词： 鼻息肉 FOXP3 T淋巴细胞亚群 CD4~+T细胞 CD8~+T细胞　出处：《郑州大学》2010年硕士论文　论文类型：学位论文

【摘要】： 背景和目的鼻息肉(nasal polyps, NP)是鼻腔和鼻窦粘膜的慢性炎症性疾病,发病率约占总人数的1%～4%。临床上以慢性鼻-鼻窦炎伴鼻息肉(CRSwNP)较为多见,其复发率较高。发病机制目前尚不清楚,近年来的研究表明可能与感染、变态反应、免疫及细胞因子等有关。叉头状转录因子3(forkhead transcription factor p3, Foxp3)是一个新发现的转录调节因子,对CD4+ CD25+ T调节性细胞的发育及功能的维持至关重要,并具有免疫抑制作用。FOXP3已被证明在CD4+ CD25+ T调节性细胞上特异性表达,在一定程度上反映CD4+ CD25+ T调节性细胞的水平和功能活性。本研究旨在分析FOXP3、CD4和CD8在鼻息肉中的表达及分布、弄清FOXP3、与CD4和CD8的相互关系,探讨FOXP3+ CD4+ CD25+ T调节细胞、T淋巴细胞亚群在鼻息肉发生、发展中的作用,明确其临床意义。材料与方法本研究对实验组50例鼻息肉组织和对照组20例正常下鼻甲黏膜组织的标本切片分别行HE染色,采用免疫组化SP法检测两组标本中FOXP3、CD4以及CD8的表达情况,采用SPSS16.0统计软件,选择正确的检验方法,进行数据分析及对照研究。结果 1 FOXP3蛋白的阳性产物主要定位于细胞核和(或)细胞质,呈棕黄色或棕褐色颗粒。免疫组化结果光镜下见：鼻息肉组和正常下鼻甲黏膜组均能观察到FOXP3阳性细胞。 2 CD4和CD8蛋白阳性产物主要定位于细胞膜,呈棕黄色或棕褐色颗粒。在鼻息肉组织中见大量的CD4和CD8阳性表达的细胞,呈丛集性分布,多集中在上皮下和腺体周围,CD4+T细胞数显著高于CD8+T细胞数。正常下鼻甲黏膜组织中大部分切片也可见少量的CD4+T细胞和CD8+T细胞。 3计算机图像分析系统检测FOXP3、CD4、CD8平均灰度值 (1)FOXP3在50例鼻息肉组织和20例正常下鼻甲黏膜组织中表达的平均灰度值差异具有统计学意义(p0.05)。在单侧和双侧鼻息肉组织中FOXP3表达的平均灰度值差异具有统计学意义(p0.05)。FOXP3在鼻息肉首发组与复发组表达的平均灰度值差异具有统计学意义(p0.05)。 (2)CD4在50例鼻息肉组织和20例正常下鼻甲黏膜组织中表达的平均灰度值差异具有统计学意义(p0.01)。在单侧与双侧鼻息肉组织中CD4表达的平均灰度值差异具有统计学意义(p0.05)。CD4在鼻息肉首发组与复发组表达的平均灰度值差异具有统计学意义(p0.05)。 (3)CD8在50例鼻息肉组织和20例正常下鼻甲粘膜组织中表达的平均灰度值差异具有统计学意义(p0.05)。在单侧与双侧鼻息肉组织中CD8表达的平均灰度值差异具有统计学意义(p0.05)。CD8在鼻息肉首发组与复发组表达的平均灰度值差异具有统计学意义(p0.05)。 4通过spearson相关分析,在鼻息肉组织中CD4与FOXP3之间存在负相关关系(γ=-0.383,p0.01)；CD8与FOXP3之间存在负相关关系(r=-0.364,p0.01)。结论 1FOXP3在鼻息肉组织中表达下调,提示FOXP3的缺乏或功能低下在鼻息肉的发生和发展中起着重要的作用。 2FOXP3、CD4和CD8均参与鼻息肉的形成过程,且可能鼻息肉的复发特性有关。 3FOXP3、CD4和CD8在鼻息肉中的表达具有相关性,提示三种蛋白可能协同参与鼻息肉的形成过程。 4检测鼻息肉组织中FOXP3表达,可以了解鼻息肉微环境中CD4+ CD25+调节性T细胞浸润状态,从而可判定鼻息肉组织局部免疫反应状态,为鼻息肉提供新的治疗方法。
[Abstract]:Background and purpose
Nasal polyps (nasal polyps NP) is a chronic inflammatory disease of nasal cavity and paranasal sinus mucosa, the incidence rate of about 1% of the total number of ~ 4%. in clinical chronic nasal sinusitis with nasal polyps (CRSwNP) is more common, the high recurrence rate. The pathogenesis is still unclear. Recent studies have shown that abnormal may react with infection, immunity and cytokines and so on.
Forkhead transcription factor 3 (forkhead transcription factor P3, Foxp3) is a newly discovered transcription factor, regulating vital to maintain the development and function of cells of CD4+ CD25+ T, and has the immunosuppressive effect of.FOXP3 has been shown to regulate the expression of cell specific CD4+ CD25+ in T, and reflect the level of functional activity of CD4+ CD25+ T regulatory cells in a certain extent.
The purpose of this study is to analyze the expression and distribution of FOXP3, CD4 and CD8 in nasal polyps, clarify the relationship between FOXP3 and CD4 and CD8, and to explore the role of FOXP3+ CD4+ CD25+ T regulating cells and the subsets of T lymphocytes in the occurrence and development of nasal polyps, and to clarify their clinical significance.
Materials and methods
In this study, the experimental group 50 cases of nasal polyps and 20 cases of control group specimens under normal turbinate mucosa were stained with HE, were detected by immunohistochemistry SP method in two groups. FOXP3, CD4 and CD8 expression, using SPSS16.0 statistical software, choose the correct test method of data analysis and the control.
Result
1, the positive products of FOXP3 protein were mainly located in nuclei and / or cytoplasm. They were brown or brown. Immunohistochemistry showed that FOXP3 positive cells could be observed in nasal polyps and normal inferior turbinate mucosa.
2 CD4 and CD8 protein was mainly expressed in the cell membrane, brownish yellow or brown particles. See CD4 and CD8 positive expression of a large number of cells in nasal polyps, a cluster distribution, mostly concentrated in the surrounding epithelium and gland, CD4+T cell number was significantly higher than that of CD8+T cells in normal mucosa tissues. In most sections of a small amount of CD4+T cells and CD8+T cells.
3 computer image analysis system to detect the average gray value of FOXP3, CD4, CD8
(1) the average gray level expression of FOXP3 in 50 cases of nasal polyps and 20 cases of normal mucosa in the value of the difference was statistically significant (P0.05). In the unilateral and bilateral nasal polyps in the average gray value of FOXP3 expression difference was statistically significant (P0.05.FOXP3) in the first group and the average gray nasal polyp recurrence group the expression of the value of the difference was statistically significant (P0.05).
(2) the average gray level expression of CD4 in 50 cases of nasal polyps and 20 cases of normal mucosa in the value of the difference was statistically significant (P0.01). In the unilateral and bilateral nasal polyps in the average gray value of CD4 expression difference was statistically significant (P0.05) the average gray.CD4 in the first group and the recurrence of nasal polyps expression of the value of the difference was statistically significant (P0.05).
(3) the average gray level expression of CD8 in 50 cases of nasal polyps and 20 cases of normal mucosa in the value of the difference was statistically significant (P0.05). In the unilateral and bilateral nasal polyps in the average gray value of CD8 expression difference was statistically significant (P0.05) the average gray.CD8 in the first group and the recurrence of nasal polyps expression of the value of the difference was statistically significant (P0.05).
4, through spearson correlation analysis, there was a negative correlation between CD4 and FOXP3 (=-0.383, P0.01) in nasal polyps, and there was a negative correlation between CD8 and FOXP3 (r=-0.364, P0.01).
conclusion
The expression of 1FOXP3 is downregulated in the nasal polyps, suggesting that the lack or infunction of FOXP3 plays an important role in the occurrence and development of nasal polyps.
2FOXP3, CD4 and CD8 are involved in the formation of nasal polyps, and may be related to the recurrent characteristics of nasal polyps.
The expression of 3FOXP3, CD4 and CD8 in nasal polyps is related, suggesting that the three proteins may be involved in the formation of nasal polyps.
4 detecting the expression of FOXP3 in nasal polyps, we can understand the infiltration state of CD4+ CD25+ regulatory T cells in the nasal polyp microenvironment, so that we can determine the local immune response state of nasal polyps, and provide a new treatment for nasal polyps.

【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R765.25

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