中耳胆脂瘤上皮中CYLD、c-jun、Ki-67的表达及意义

发布时间：2018-03-03 00:25

本文选题：中耳胆脂瘤　切入点：增殖　出处：《中华耳科学杂志》2014年04期 　论文类型：期刊论文

【摘要】：目的研究抑癌基因CYLD(cylindromatosis)、原癌基因c-jun及细胞核增殖相关抗原Ki-67在中耳胆脂瘤上皮细胞中的表达情况,分析它们之间的相互关系,探讨CYLD、c-jun、Ki-67在中耳胆脂瘤发病机制中的可能作用。方法采用免疫组化技术检测CYLD、c-jun及Ki-67蛋白产物在30例中耳胆脂瘤上皮组织(实验组)及20例正常耳后皮肤上皮组织(对照组)中的表达情况。结果 CYLD蛋白产物阳性表达定位于细胞浆,其在中耳胆脂瘤上皮中主要见基底层弱阳性或阳性表达,而在对照组正常耳后皮肤上皮全层均见阳性表达或强阳性表达,CYLD蛋白在中耳胆脂瘤上皮中的表达明显低于正常耳后皮肤(X2=16.333,p=0.000,p0.05)。c-jun蛋白产物阳性表达定位于细胞浆和细胞核,其在中耳胆脂瘤上皮中上皮全层均见阳性表达或强阳性表达,而在对照组正常耳后皮肤上皮中均仅见基底层弱阳性或阳性表达,c-jun蛋白在中耳胆脂瘤上皮中的表达明显高于正常耳后皮肤(X2=14.901,p=0.000,p0.05)。Ki-67蛋白产物定位于细胞核,细胞质内未见Ki-67蛋白表达。其在中耳胆脂瘤上皮基底层、棘层,甚至颗粒层中均可见阳性或强阳性表达,而在对照组正常耳后皮肤上皮中均仅见基底层弱阳性或阳性表达,Ki-67蛋白在中耳胆脂瘤上皮中的表达明显高于正常耳后皮肤(X2=13.675,P=0.000,P0.05)。Spearman相关性分析发现中耳胆脂瘤上皮细胞CYLD、c-jun蛋白表达存在负相关(rs=-0.381,P=0.038,P0.05)。结论与正常耳后皮肤的上皮细胞相比,中耳胆脂瘤上皮细胞具有高度增殖的能力。CYLD、c-jun异常表达可能与中耳胆脂瘤发生、发展密切相关。在中耳胆脂瘤上皮中可能也存在CYLD对c-jun表达的负调节。
[Abstract]:Objective to study the expression of CYLDD Cylindromatosisus, proto-oncogene c-jun and nuclear proliferation-associated antigen (Ki-67) in middle ear cholesteatoma epithelial cells, and to analyze the relationship between them. To explore the possible role of CYLDX c-juni Ki-67 in the pathogenesis of middle ear cholesteatoma. Methods Immunohistochemical technique was used to detect CYLD- c-jun and Ki-67 protein products in 30 cases of middle ear cholesteatoma epithelium (experimental group) and 20 cases of normal retroauricular skin (P < 0.05). Results the positive expression of CYLD protein was located in the cytoplasm. In the middle ear cholesteatoma epithelium, weak positive or positive expression was found in the basal layer. The positive expression of CYLD protein in the epithelium of middle ear cholesteatoma was significantly lower than that in the normal skin of the control group. The positive expression of CYLD protein was located in cytoplasm and nucleus. Positive or strong positive expression was found in the whole epithelium of middle ear cholesteatoma. In control group, the expression of c-jun protein was significantly higher in the epithelium of middle ear cholesteatoma than that in the normal skin of the normal ear. The protein products of X2P 14.901 P0. 000 p0. 05 and Ki-67 were located in the nucleus of the control group, and the expression of c-jun protein in the epithelium of middle ear cholesteatoma was higher than that in the normal skin of the normal ear. There was no expression of Ki-67 protein in cytoplasm. Positive or strong expression of Ki-67 protein was found in basal layer, spinous layer and even granular layer of middle ear cholesteatoma. In the control group, the expression of Ki-67 protein was only weakly positive or positive in the basal layer of normal posterior ear skin epithelium, and the expression of Ki-67 protein in the epithelium of middle ear cholesteatoma was significantly higher than that in normal retroauricular skin skin. The correlation analysis showed that the epithelial cells of middle ear cholesteatoma were higher than that of normal skin. The expression of CYLDN c-jun protein was negatively correlated with that of the normal skin epithelial cells, and the expression of CYLDN c-jun protein was negatively correlated with that of the normal skin epithelium, and the expression of CYLDD c-jun protein was negatively correlated with that of the normal skin. The abnormal expression of CYLDN c-jun may be closely related to the occurrence and development of middle ear cholesteatoma. There may also be a negative regulation of c-jun expression by CYLD in middle ear cholesteatoma epithelium.
【作者单位】：中山大学附属第六医院耳鼻咽喉头颈外科;暨南大学附属第一医院耳鼻咽喉头颈外科;
【分类号】：R764.21

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