雷公藤红素治疗变应性鼻炎大鼠的机制研究

发布时间：2018-03-04 23:33

本文选题：雷公藤红素　切入点：鼻炎　出处：《临床耳鼻咽喉头颈外科杂志》2014年08期 　论文类型：期刊论文

【摘要】：目的:研究雷公藤红素对大鼠变应性鼻炎(AR)的治疗作用并初步探讨相关作用机制。方法:构建大鼠AR模型,测定大鼠第1天、第4天和第7天的行为学特征改变、鼻黏膜呼吸区组织超氧化物歧化酶(SOD)、丙二醛(MDA)、还原型谷胱甘肽(GSH)和谷胱甘肽过氧化物酶(GSH-PX)的水平;测定鼻黏膜呼吸区组织核因子E2p45相关因子2(NRF2)的核内蛋白表达及谷氨酸半胱氨酸连接酶催化亚基(GCLC)的浆蛋白表达。结果:AR大鼠第1、4、7天的行为学特征改变显著(P0.01),鼻黏膜呼吸区组织SOD、GSH、GSH-PX降低,MDA升高;NRF2的核内蛋白表达降低,GCLC的浆蛋白表达降低。雷公藤红素低剂量治疗组和高剂量治疗组能不同程度改善上述指标的变化,同时提高了鼻黏膜呼吸区组织NRF2的核蛋白表达和GCLC的浆蛋白表达。结论:雷公藤红素能改善AR大鼠的症状,其机制可能与提高鼻黏膜呼吸区组织NRF2的核蛋白表达和GCLC的浆蛋白表达相关。
[Abstract]:Objective: To study the effect of tripterine on rats with allergic rhinitis (AR) treatment and explore the related mechanism. Methods: the AR rat model, rats were first days, fourth days and seventh days of behavior change, nasal respiratory tissue superoxide dismutase (SOD). Malondialdehyde (MDA), glutathione (GSH) and glutathione peroxidase (GSH-PX) levels; Determination of nasal respiratory tissue nuclear factor E2p45 related factor 2 (NRF2) of the nuclear protein expression and glutamate cysteine ligase catalytic subunit (GCLC) expression of plasma protein. Results: the behavior of day 1,4,7 AR the characteristics of rats changed significantly (P0.01), nasal respiratory tissue SOD, GSH, GSH-PX decreased, MDA increased; the expression of nuclear protein NRF2 decreased expression of GCLC protein decreased. The plasma tripterine low dose group and high dose treatment group can be improved on Changes in the index, while improving the nuclear protein expression and GCLC nasal respiratory tissue NRF2 plasma protein expression. Conclusion: celastrol can improve the symptoms of AR rats, and its mechanism may improve nuclear protein expression and GCLC nasal respiratory tissue NRF2 plasma protein expression.

【作者单位】：邢台市人民医院耳鼻咽喉科;邢台市人民医院普外科;邢台市人民医院胸外科;
【分类号】：R765.21

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