原发性青光眼合并2型糖尿病患者房水及血液中MMP-2及TIMP-2的表达
本文选题：原发性青光眼 切入点：型糖尿病 出处：《眼科新进展》2015年02期 论文类型：期刊论文
【摘要】：目的探讨基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)及金属蛋白酶2组织抑制因子(tissue inhibitor of metalloproteinase-2,TIMP-2)与原发性青光眼合并2型糖尿病的关系。方法研究对象分A组、B组、C组、D组、E组、F组6组,每组30眼。A组为原发性开角型青光眼(primary open angle glaucoma,POAG)组,B组为原发性闭角型青光眼(primary angle closure glaucoma,PACG)组,C组为POAG合并2型糖尿病组,D组为PACG合并2型糖尿病组,E组为糖尿病性白内障组,F组为年龄相关性白内障组。采用酶联免疫吸附试验检测各组房水及血清中TIMP-2及MMP-2的含量,并计算TIMP-2/MMP-2值。结果在6组研究对象的房水中,MMP-2浓度在A组为(24.92±6.62)μg·L-1、B组为(36.80±15.07)μg·L-1、D组为(28.44±5.78)μg·L-1,均较F组的(22.87±3.54)μg·L-1显著升高(均为P0.05);同时房水中TIMP-2浓度在A组为(43.92±19.57)μg·L-1、B组为(76.13±27.67)μg·L-1、D组为(61.92±6.51)μg·L-1,也均较F组的(22.48±3.56)μg·L-1显著升高(均为P0.05)。A、B、C、D组房水中TIMP-2/MMP-2值较E组和F组显著提高,约为其2倍,但A组、B组、C组、D组间TIMP-2/MMP-2值无显著性差异。在6组研究对象的血清中,A组MMP-2和TIMP-2浓度最高,分别为(396.75±49.30)μg·L-1和(337.67±62.78)μg·L-1,其余各组间MMP-2和TIMP-2浓度无显著性差异。6组研究对象血清中TIMP-2/MMP-2值无明显差异。结论原发性青光眼患者中TIMP-2/MMP-2值均存在失衡,说明MMP-2的活性变化及TIMP-2/MMP-2值与POAG和PACG的发病密切相关,但2型糖尿病对原发性青光眼的病程进展无明显影响。
[Abstract]:Objective to investigate the relationship between matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) and primary glaucoma complicated with type 2 diabetes mellitus. 30 eyes of group A: primary open angle glaucoma: primary open angle glaucoma group (group B, primary angle closure glaucoma, primary angle closure glaucoma group), group C, POAG combined with type 2 diabetes, group D, PACG, combined with type 2 diabetes, group E, diabetic whitening, group B, group B: primary angle closure glaucoma, primary angle closure glaucoma group, group C, group C, POAG combined with type 2 diabetes, group D, PACG, combined with type 2 diabetes, group B, group B, primary angle closure glaucoma. The contents of TIMP-2 and MMP-2 in aqueous humor and serum were detected by enzyme linked immunosorbent assay (Elisa). Results the concentration of MMP-2 in aqueous humor in group A was 24.92 卤6.62 渭 g 路L -1, and that in group B was 36.80 卤15.07 渭 g 路L -1, which was 28.44 卤5.78 渭 g 路L -1, which was significantly higher than that in group F (22.87 卤3.54) 渭 g 路L -1, and the concentration of TIMP-2 in aqueous humor was 43.92 卤19.57 渭 g 路L -1 in group A, 76.13 卤27.67 渭 g 路L -1 in group B, 61.92 卤6.51 渭 g 路L -1 in group D and 61.92 卤6.51 渭 g 路L -1 in group D, respectively. The concentration of TIMP-2/MMP-2 in aqueous humor of group D was significantly higher than that of group E and group F (P 0.05 卤3.56) 渭 g 路L -1. But there was no significant difference in TIMP-2/MMP-2 between C and D groups. The serum MMP-2 and TIMP-2 concentrations of group A were the highest among the six groups. The concentrations of MMP-2 and TIMP-2 were 396.75 卤49.30 渭 g 路L -1 and 337.67 卤62.78 渭 g 路L -1, respectively. There was no significant difference in the concentration of MMP-2 and TIMP-2 between other groups. The results showed that the changes of MMP-2 activity and TIMP-2/MMP-2 value were closely related to the onset of POAG and PACG, but type 2 diabetes had no significant effect on the progression of primary glaucoma.
【作者单位】： 赣南医学院第一附属医院眼科;
【基金】：江西省科技支撑计划项目(编号:20122BBG70156-1)~~
【分类号】：R775;R587.1
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