错配修复基因hMLH1和hMSH2在下咽癌及切缘中的表达及临床意义

发布时间：2018-03-05 22:30

  本文选题：hMLH1　切入点：hMSH2　出处：《河南科技大学》2011年硕士论文　论文类型：学位论文

【摘要】：目的:检测下咽癌及切缘和正常喉咽组织中hMLH1、hMSH2的表达状况,揭示其与下咽癌发生发展预后的关系,以期为下咽癌的早期诊断、判断预后和基因治疗及其预防提供理论依据,并探讨下咽癌手术的安全切缘。 方法:本研究采用免疫组化SP法,从2008-2010年手术治疗的不同分期分级的下咽癌患者中随机选取50例,每例取癌组织、表面癌旁3mm,5mm,10mm石蜡标本切片,并取20例正常喉组织做对照,检测下咽癌及切缘和正常咽组织中hMLH1和hMSH2的表达状况,由此探讨其与下咽癌发生发展和预后的关系,以及判定下咽癌手术的安全切缘范围。 结果: 1.hMLH1与hMSH2蛋白阳性表达主要位于细胞核,为棕褐色或黄色,一少部分位于细胞浆即核浆型,着色较浅且分布均匀。 2.在下咽癌和正常喉组织中hMLH1蛋白的阳性率分别为54%和80%,两组间表达差异具有统计学意义(P0.05);hMSH2蛋白的阳性率分别为56%和85%,两组间表达差异差异具有统计学意义(P0.05)。 3.hMLH1与hMSH2蛋白的表达和下咽癌患者性别、年龄和临床分期无明显相关(P 0.05)。 4.hMLH1与hMSH2蛋白的表达与下咽癌的分化程度和淋巴结转移有明显相关(P0.05)。 5.在下咽癌组织中hMLH1与hMSH2蛋白的表达呈正相关,r=0.713(P0.05)。 6. hMLH1与hMSH2蛋白的阳性表达按癌组织、癌旁3mm、5mm、10mm和正常喉组织的顺序依次递增,越靠近正常组织,阳性表达越高。差异具有显著性(P0.05)。 结论:1.下咽癌细胞中存在hMLH1与hMSH2基因蛋白的表达下调,hMLH1与hMSH2基因蛋白的缺失,可能参与了下咽癌早期的发生过程。 2.hMLH1与hMSH2基因蛋白的表达与下咽癌患者性别、年龄、临床分期无相关性。 3.hMLH1与hMSH2基因蛋白的表达与下咽癌的分化程度、淋巴结转移相关。 4.下咽癌组织中hMLH1、hMSH2蛋白的表达呈正相关,hMLH1与hMSH2可能一起参与了下咽癌的发生、发展、侵袭及转移的过程。 5.hMLH1与hMSH2有可能成为下咽癌预警的标记物。 6.在下咽癌中检测hMLH1与hMSH2基因蛋白,将可为研究下咽癌的发生发展及其基因治疗提供理论依据。 7.下咽癌手术可以选择距肿瘤10mm作为安全切缘参考标准。
[Abstract]:Objective: to detect the expression of hMLH1hMSH2 in hypopharyngeal carcinoma and its incisal margin and normal larynx tissue, and to reveal the relationship between hMLH1hMSH2 and the prognosis of hypopharyngeal carcinoma, so as to provide theoretical basis for early diagnosis, judgement of prognosis, gene therapy and prevention of hypopharyngeal carcinoma. And to explore the safe margin of hypopharyngeal carcinoma surgery. Methods: in this study, 50 patients with hypopharyngeal carcinoma were randomly selected from 50 patients with hypopharyngeal carcinoma in different stages and grades treated by operation from 2008 to 2010 by immunohistochemical SP method. Each case was taken from the cancer tissue, and the paraffin sections of 3 mm to 5 mm or 10 mm adjacent to the surface carcinoma were taken, and 20 cases of normal laryngeal tissue were taken as control. To detect the expression of hMLH1 and hMSH2 in hypopharyngeal carcinoma and its incisal margin and normal pharyngeal tissue, and to explore the relationship between the expression of hMLH1 and hMSH2 and the occurrence, development and prognosis of hypopharyngeal carcinoma, and to determine the safe margin of surgery for hypopharyngeal carcinoma. Results: 1. The positive expression of hMLH1 and hMSH2 protein was mainly located in the nucleus, which was brown or yellow, and a few in the cytoplasm, the staining was light and evenly distributed. 2. 2. The positive rates of hMLH1 protein in hypopharyngeal carcinoma and normal larynx were 54% and 80, respectively. The positive rates of hMLH1 protein were 56% and 85 in hypopharyngeal carcinoma and normal larynx, respectively. The difference between the two groups was statistically significant (P 0.05). 3. There was no significant correlation between hMLH1 expression and hMSH2 protein expression, sex, age and clinical stage of patients with hypopharyngeal carcinoma (P 0.05). 4. The expression of hMLH1 and hMSH2 protein was significantly correlated with the degree of differentiation and lymph node metastasis of hypopharyngeal carcinoma. 5. There was a positive correlation between the expression of hMLH1 and hMSH2 protein in hypopharyngeal carcinoma tissues. 6. The positive expression of hMLH1 and hMSH2 protein increased in the order of cancer tissue, adjacent 3 mm ~ 5 mm ~ 10 mm and normal laryngeal tissue. The closer the tissue was to the normal tissue, the higher the positive expression was, and the difference was significant (P 0.05). Conclusion the expression of hMLH1 and hMSH2 gene protein down-regulated the deletion of hMLH1 and hMSH2 gene protein in hypopharyngeal carcinoma cells, which may be involved in the early development of hypopharyngeal carcinoma. 2. The expression of hMLH1 and hMSH2 gene protein had no correlation with sex, age and clinical stage of patients with hypopharyngeal carcinoma. 3. The expression of hMLH1 and hMSH2 gene protein was correlated with the differentiation of hypopharyngeal carcinoma and lymph node metastasis. 4. The expression of hMLH1hMSH2 protein was positively correlated with the expression of hMLH1hMSH2 protein in hypopharyngeal carcinoma tissues. HMLH1 and hMSH2 may be involved in the process of occurrence, development, invasion and metastasis of hypopharyngeal carcinoma. 5. HMLH1 and hMSH2 may be markers for early warning of hypopharyngeal cancer. 6. Detection of hMLH1 and hMSH2 gene proteins in hypopharyngeal carcinoma may provide theoretical basis for the study of the occurrence and development of hypopharyngeal carcinoma and its gene therapy. 7. For hypopharyngeal carcinoma surgery, 10 mm away from the tumor can be selected as the reference standard for the safe margin of resection.
【学位授予单位】：河南科技大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.64

【参考文献】

相关期刊论文 前10条

1 游龙贵,孙炜,孙彦,张克辉,张小安,兰玉清;喉癌手术切缘Bcl-2、Bax及Bak蛋白的表达[J];青岛大学医学院学报;2005年01期

2 谷建琦;张英怀;董青;贾志宇;;hMLH1、hMSH2蛋白在口腔鳞状细胞癌中的表达及意义[J];北京口腔医学;2007年02期

3 周琪,阎晓初,辛榕,段光杰,章容,柳凤轩;大肠腺瘤及其癌变错配修复基因hMLH1和hMSH2表达与细胞增殖的关系[J];第三军医大学学报;2004年07期

4 赵坚强;;下咽癌的手术治疗回顾与展望[J];临床医学;2006年07期

5 郭成;车向明;刘青光;;hMSH2,hMLH1的表达与胃癌临床病理及预后的关系[J];广东医学;2008年01期

6 杨胜利,谭家驹,杨光;DNA损伤修复基因多态性与食管癌的研究进展[J];国外医学.外科学分册;2005年03期

7 李佩玲,刘梅梅;错配修复基因hMSH2研究的新进展[J];国外医学.遗传学分册;2002年04期

8 范凯;马坚妹;王朝晖;王彦;刘敏;吕申;许国旺;;胃癌中hMSH2、PTEN和PCNA表达的相关性及意义[J];解剖学杂志;2006年01期

9 李为民,郭睿,郭志祥;喉癌手术切缘的组织病理学研究[J];临床耳鼻咽喉科杂志;1998年11期

10 孙越峰;喉癌手术切缘的研究进展[J];临床耳鼻咽喉科杂志;2001年09期

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