蛴螬提取物对实验性兔视网膜静脉阻塞新生血管因子HGF、ES表达影响的研究

发布时间：2018-03-11 02:09

本文选题：视网膜静脉阻塞　切入点：视网膜新生血管　出处：《湖南中医药大学》2010年硕士论文　论文类型：学位论文

【摘要】：目的评价蛴螬提取物对有色家兔视网膜静脉阻塞的治疗作用和防治并发症新生血管(RNV)的机制。方法40只有色兔随机份为4组,即正常对照组(A组)、实验对照组(B组)、复方血栓通治疗组(C组)、蛴螬提取物治疗组(D组),每组10只兔20只眼,A组用蒸馏水灌胃,B、C、D组用光化学动力法建立RVO动物模型,分别用蒸馏水、复方血栓通片、蛴螬提取物连续灌胃,于造模后1h,1d,1wk,2wk,3wk行眼底照相,于造模后1d、1wk、2wk、3wk行FFA检查,于造模后1wk、3wk行HE染色、免疫组化HGF、ES染色及其结果观察分析。结果B、C、D三组60只眼成功建立了RVO模型。造模后1wk,各造模组眼底均见出血,无灌注区面积和视盘面积比值B组最大,C组居中,D组最小；造模后2wk,各组仍见水肿、出血及无灌注区,无灌注区面积和视盘面积比值B组增大,且10眼3眼出现新生血管,C、D组面积比值减小；造模后3wk,B组10眼5眼出现新生血管,其无灌注区面积扩大,C、D组无新生血管出现,未见明显无灌注区。造模后1wk和3wk,视网膜切片光镜观察结果显示：D组视网膜各层结构病理变化均轻于B、C组,HGF、ES免疫组化结果示：HGF含量B组较C、D组显著上升,D组上升最小,ES含量B组较C、D组显著下降,D组下降最小,差异有统计学意义(P0.05)。结论蛴螬提取物能促进实验性RVO模型出血的吸收,改善视网膜缺血缺氧的状态,可以抑制实验性RVO模型中HGF的表达,促进ES的表达,从而可以抑制实验性RNV的形成。
[Abstract]:Objective to evaluate the therapeutic effect of grub extract on retinal vein occlusion and the mechanism of prevention and treatment of RNVs. Methods 40 colored rabbits were randomly divided into 4 groups. The RVO animal model was established by photochemical method in group A (n = 20), group A (n = 20) in each group (n = 10), group A (n = 20), group A (n = 20), group A (n = 20), group A (n = 20), group A (n = 10) were treated with distilled water. Distilled water, compound Xuetong tablet and grub extract were continuously perfused into the stomach. Fundus photography was performed at 1 hour after model making, 1 wk at 1wk and 2wk at 3wk after modeling. FFA was performed at 1wk after modeling, HE staining was performed at 1wk 3 wk after modeling, HGFES staining was performed and the results were observed and analyzed. Results the RVO model was successfully established in 60 eyes of group D, and hemorrhage was found in the fundus of each model group 1 wk after the model was made, the largest ratio of no perfusion area to optic disc area in group B was the smallest in group D, and edema was found in each group at 2 wk after modeling. The ratio of no perfusion area to optic disc area increased in group B and decreased in 3 eyes of 10 eyes and 5 eyes in group B 3 weeks after modeling. No neovascularization was found in group C ~ (2 +) with enlarged area of no perfusion area. There was no obvious no perfusion area. 1 wk and 3 wk after modeling, the pathological changes of each layer of retina in group B were lighter than those in group B and group C. The immunohistochemical results showed that the content of HGF in group B was significantly higher than that in group C and group D was higher than that in group C. The minimum es content in group B was significantly lower than that in group C D and that in group D was lower than that in group D. The difference was statistically significant (P 0.05). Conclusion Grub extract can promote the absorption of hemorrhage in experimental RVO model, improve the state of retinal ischemia and hypoxia, inhibit the expression of HGF and es in experimental RVO model, and thus inhibit the formation of experimental RNV.
【学位授予单位】：湖南中医药大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R774.1

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