鼻内翻性乳头状瘤与HPV感染及其亚型的关系

发布时间：2018-03-12 13:50

本文选题：内翻性乳头状瘤　切入点：人乳头状瘤病毒　出处：《宁夏医科大学》2010年硕士论文　论文类型：学位论文

【摘要】：目的探讨人乳头状瘤病毒(Human Papillomavirus,HPV)在鼻内翻性乳头状瘤(Nasal Inverted Papilloma,NIP)中感染亚型的分布及其在病变发生、发展中的作用。方法取101例NIP石蜡包埋组织标本采用核酸分子快速导流杂交基因芯片分型技术(HybriMax)对21种HPV亚型同步进行检测和病毒分型,并以24例健康志愿者鼻腔黏膜组织为对照。结果NIP石蜡标本HPV总阳性率64.36%(65/101)。对照组HPV均为阴性(0/24)。两组比较差异有统计学意义(χ2=32.178,P0.05)。单纯NIP组、伴不典型增生NIP组、癌变组HPV阳性率分别为59.74%(46/77)、81.82%(18/22)、50%(1/2)。单纯NIP组与伴不典型增生NIP组的HPV感染率比较差异无统计学意义(χ2=3.649,P =0.056)。单纯NIP组与伴不典型增生NIP组的HPV高危亚型、低危亚型的构成比差异有统计学意义(χ2=10.412,P0.05)。参照Krouse分期体系,101例NIP分为四组,即KrouseI-IV期,四组HPV阳性率分别为45.45%(10/22)、62.50%(10/56)、89.47%(17/19)、75.00%(3/4),四组不同临床分期的NIP中的HPV感染率差异有统计学意义(χ2=8.349,P0.05)。结论NIP的发病与HPV感染密切相关,HPV11、58、16、44型可能是与国人鼻内翻性乳头状瘤发生发展相关的主要亚型。HPV高危亚型或多重亚型混合感染能促使NIP向不典型增生发展,HPV感染可能使NIP病变范围扩大。明确NIP中HPV亚型的分布情况对肿瘤临床行为的预测有一定的指导意义。
[Abstract]:Objective to investigate the distribution of human papillomavirus (HPV) infection subtype in nasal inverted papillomavirus (nipp) and its role in the pathogenesis and development of human papillomavirus (HPV). Methods Twenty-one HPV subtypes and virus typing were detected in 101 NIP paraffin-embedded tissue samples by hybridization hybridization gene typing technique and 24 healthy volunteers were used as control. Results the total positive rate of HPV in paraffin wax specimens of NIP was 64.36% and 65 / 101% respectively. The HPV of control group was negative 0 / 24%. There was a significant difference between the two groups (蠂 2 + 32.178% P 0.05). In simple NIP group, NIP group with atypical hyperplasia, there was a significant difference between the two groups. The positive rates of HPV in cancer group were 59.74 / 46 / 77 / 81.82 and 18 / 22 / 50 / 2 respectively. There was no significant difference in HPV infection rate between simple NIP group and NIP group with atypical hyperplasia (蠂 ~ (2) 3.649) P ~ (0.056). The high risk subtypes of HPV in simple NIP group and NIP group with atypical hyperplasia were not significantly different. According to the Krouse staging system, 101 cases of NIP were divided into four groups: KrouseI-IV stage. The positive rates of HPV in the four groups were 45.4510 / 10 / 22 / 62.50 / 10 / 56 / 10.47 / 1975.00 / 3 / 4, respectively (蠂 ~ 28.349 / P 0.05), and there was a significant difference in HPV infection rate among the four groups (蠂 ~ 28.349 / P 0.05). Conclusion the pathogenesis of NIP is closely related to the infection of HPV. HPV1158 / 1644 may be the main subtype associated with the occurrence and development of Chinese nasal inverted papilloma. High risk subtype or multiple subtypes of HPVinfection may promote the development of NIP towards atypical hyperplasia. The distribution of HPV subtypes in NIP may be helpful to predict the clinical behavior of NIP.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.62

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