地塞米松对低渗诱导的人小梁细胞容积敏感性氯电流的影响

发布时间：2018-03-14 00:17

本文选题：小梁细胞　切入点：氯化物通道　出处：《吉林大学学报(医学版)》2014年06期 　论文类型：期刊论文

【摘要】：目的:分析地塞米松对低渗诱导的人小梁细胞容积敏感性氯电流的影响,探讨容积敏感性氯通道(VACC)在激素性青光眼(GIG)发病中的可能机制。方法:人小梁细胞悬液接种于直径35mm塑料培养皿中单层生长,按培养液中是否含地塞米松分组,即正常培养液组和地塞米松培养1、3和7d组,共4组,采用全细胞膜片钳技术分别记录4组细胞在低渗状态下的氯电流密度值,比较各组数值的差异。结果:正常培养液组小梁细胞低渗刺激后,在+100和-100 mV电压钳钳制下,外向和内向电流密度值分别为(19.94±0.87)和(-6.53±0.41)pA/pF。地塞米松培养1、3和7d组的小梁细胞低渗刺激后,在+100和-100mV电压钳钳制下,外向和内向电流密度值分别为(19.39±1.40)和(-6.42±0.28)pA/pF、(17.97±2.35)和(-5.82±0.94)pA/pF、(17.16±1.16)和(-5.65±0.43)pA/pF。地塞米松培养1d组小梁细胞较正常培养液组低渗刺激后的外向和内向电流密度值减小,但差异无统计学意义(P0.05);地塞米松培养3和7d组小梁细胞较正常培养液组小梁细胞低渗刺激后的外向和内向电流密度值明显减小(P0.05)。结论:地塞米松导致体外培养的正常人小梁细胞容积敏感性氯电流密度值降低,进而影响小梁细胞容积调节,可能是导致GIG房水流出阻力增加的原因之一。
[Abstract]:Objective: to investigate the effect of dexamethasone on volume-sensitive chloride currents in hypotonic induced human trabecular cells. To investigate the possible mechanism of volume-sensitive chloride channel (VAC) in the pathogenesis of steroid-induced glaucoma (GIG.Methods: human trabecular cell suspension was inoculated in 35 mm diameter plastic dish for monolayer growth and divided into groups according to whether dexamethasone was contained in the culture medium or not. Normal culture medium group and dexamethasone culture group for 3 and 7 days were used to record the chlorine current density of 4 groups under hypotonic condition by whole cell patch clamp technique. Results: after hypotonic stimulation of trabecular cells in the normal medium group, the trabecular cells were clamped with 100 MV and -100 MV voltage forceps. The current density of extroversion and introversion were 19.94 卤0.87) and 6.53 卤0.41pA / pF.Dexamethasone cultured trabecular meshwork cells at 100 and -100 MV voltage clamp after hypotonic stimulation for 1 d and 7 d, respectively. The outward and inward current density values were 19.39 卤1.40) and 6.42 卤0.28 Pa / pFU 17.97 卤2.35) and 5.82 卤0.94pA / pFU 17.16 卤1.16) and 5.65 卤0.43 pAr / pF.The outward and inward current densities of trabecular meshwork cells in the dexamethasone culture group were significantly lower than those in the normal medium group for 1 day, and the outward and inward current densities of the trabecular meshwork cells in the dexamethasone group were significantly lower than those in the normal medium group. However, the difference was not statistically significant (P 0.05), and that of trabecular cells in dexamethasone group for 3 and 7 days was significantly lower than that in normal medium group after hypotonic stimulation (P 0.05). Conclusion: dexamethasone can induce the effect of dexamethasone on the growth of trabecular meshwork cells in vitro. In normal human trabecular meshwork cells, the volume-sensitive chloride current density decreased, The effect of trabecular cell volume regulation may be one of the reasons for the increase of outflow resistance of GIG aqueous humor.
【作者单位】：黑龙江省哈尔滨普瑞眼科医院眼科;吉林大学第二医院眼科;
【基金】：国家自然科学基金资助课题(81271002)
【分类号】：R775

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