人类几丁质酶在慢性鼻—鼻窦炎与鼻息肉中的表达

发布时间：2018-03-18 14:23

  本文选题：几丁质酶　切入点：几丁质类蛋白　出处：《华中科技大学》2011年硕士论文　论文类型：学位论文

【摘要】：目的探讨人类几丁质酶家族Acidic Mammalian Chitinase(AMcase) , Chitotriosidase(CHIT-1), Stabilin-1 Interacting Chitinase-like Protein (SI-CLP) ,Chi3l1(Chitinase 3 like 1),CTBS(Chitobiase)和Oviductal Glycoprotein 1(OVGP-1)在正常鼻粘膜,不伴鼻息肉的慢性鼻-鼻窦炎和伴鼻息肉的慢性鼻-鼻窦炎中的表达。 方法收集10例正常下鼻甲粘膜(inferior turbinate tissues ITTs),9例不伴鼻息肉的慢性鼻-鼻窦炎(chronic rhinosinusitis CRS),9例伴鼻息肉的慢性鼻-鼻窦炎( nasal polyps NPs)患者组织标本,采用实时荧光定量PCR技术检测各组中人类几丁质酶家族成员mRNA的表达。 结果AMCase,CHIT-1,SI-CLP,Chi3l1和OVGP-1在各组中均有表达。CTBS在ITTs组中的阳性率为20%(2?10),CRSsNP中为33%(3?9)和CRSwNPs中为33% (3?9)。与ITTs组相比AMCase与Chi3l1在CRSwNPs组的表达水平明显上调,分别为2.4倍(P=0.008)和2.16倍(P=0.03)。 在ITTs组中,CHIT-1的表达和SI-CLP (r = 0.76, P = 0.0092),Chi3l1(r = 0.63, P = 0.047),OVGP-1(r = 0.87, P = 0.008)的表达正相关; Chi3l1和OVGP-1的表达(r = 0.67, P = 0.033)正相关。在CRSsNP组,CHIT-1的表达和SI-CLP (r = 0.76, P = 0.015),Chi3l1(r = 0.66, P = 0.049),AMCase(r = 0.73, P = 0.0246),OVGP-1(r = 0.93, P = 0.0002)的表达正相关; AMCase和SI-CLP (r = 0.7 , P = 0.0358)及OVGP-1(r = 0.85, P = 0.0037)的表达正相关。在CRSwNP组中CHIT-1分别和Chi3l1(r = 0.81, P = 0.0072),OVGP-1(r = 0.68, P = 0.042)的表达正相关;Chi3l1和AMCase(r =0.66 , P = 0.049),OVGP-1(r = 0.71, P = 0.00298)的表达正相关; AMCase和OVGP-1(r = 0.71, P = 0.0298)的表达正相关. 结论几丁质酶家族成员在正常以及炎性状态的鼻腔和鼻窦粘膜中有表达,部分家族成员的共表达具有显著性意义。
[Abstract]:Objective to investigate the expression of Acidic Mammalian Chitinase (Acidic Mammalian Chitinase), Chitotriosidase (CHIT-1), Stabilin-1 Interacting Chitinase-like Protein (SI-CLP) and Oviductal Glycoprotein 1OVGP-1 in normal nasal mucosa, chronic rhinosinusitis without nasal polyps and chronic rhinosinusitis with nasal polyps. Methods the tissue specimens of 9 patients with chronic rhinosinusitis without nasal polyps and 9 patients with nasal polyps sinusitis without nasal polyps were collected. The expression of mRNA in human chitinase family was detected by real-time fluorescence quantitative PCR. Results the positive rate of OVGP-1 in ITTs group was 20? In CRSsNP, there are 33? 9) and CRSwNPs for 33%? Compared with the ITTs group, the expression of AMCase and Chi3l1 were significantly up-regulated in the CRSwNPs group (2.4-fold P0. 008) and 2.16-fold Pmex 0.03 (P < 0.05). In ITTs group, there was a positive correlation between the expression of CHIT-1 and SI-CLP tir = 0.76, P = 0.0092Chi3l1tr = 0.63, P = 0.047OVGP-1tr = 0.87, P = 0.008), and the positive correlation between the expression of Chi3l1 and OVGP-1 was 0.67, P = 0.033). In CRSsNP group, the expression of CHIT-1 was positively correlated with SI-CLP tir = 0.76, P = 0.015, Chi3l1mcr = 0.66, P = 0.0499AMCASE r = 0.66. There was a positive correlation between the expression of AMCase and SI-CLP r = 0. 7, P = 0. 0358) and OVGP-1(r = 0. 85, P = 0. 0037). In the CRSwNP group, CHIT-1 was positively correlated with Chi3l1(r = 0. 81, P = 0. 0072, OVGGP-1, r = 0. 68, P = 0. 042). There was a positive correlation between the expression of AMCase and OVGP-1(r (P = 0.71, P = 0.0298), and the positive correlation between the expression of OVGP-1P = 0.71, P = 0.00298, and the expression of AMCase and OVGP-1(r = 0.71, P = 0.0298). Conclusion chitinase family members are expressed in normal and inflammatory nasal cavity and nasal sinus mucosa, and the coexpression of some family members is significant.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R765

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