低剂量顺铂对小鼠耳蜗内毛细胞形态及otoferlin蛋白的影响

发布时间：2018-03-21 17:20

本文选题：顺铂　切入点：内毛细胞　出处：《临床耳鼻咽喉头颈外科杂志》2014年11期 　论文类型：期刊论文

【摘要】：目的:建立稳定有效的低剂量顺铂损伤听觉模型,探讨顺铂造成听功能损伤的机制。方法:将C57小鼠随机分为7组,分别为正常组,不同剂量、不同时间损伤实验组,每组8只。通过小鼠听性脑干反应(ABR)阈值测量、AgNO3毛细胞形态染色、免疫荧光法、RT-PCR测定基膜底回内毛细胞otoferlin蛋白的表达等方式检测顺铂对耳蜗内毛细胞形态及otoferlin蛋白的影响。结果:在同一时间点,用药4d后,1.5mg/kg和3.0mg/kg实验组较正常组小鼠ABR阈值明显升高,用药7d的各实验组ABR阈值较正常组均明显升高。同一剂量下,0.75mg/kg×7d组较0.75mg/kg×4d组阈值明显增高,其余剂量无明显时间差异。实验组中3.0mg/kg实验组蛋白表达均降低,1.5mg/kg实验组与正常组无差异。3.0mg/kg×7d组内毛细胞形态改变最明显,但在各组均未缺失。结论:低剂量顺铂可造成听功能损伤,其损伤机制很可能与内毛细胞的损伤及otoferlin蛋白有关。
[Abstract]:Objective: to establish a stable and effective hearing model of low dose cisplatin injury and to explore the mechanism of hearing impairment induced by cisplatin. Methods: C57 mice were randomly divided into 7 groups: normal group, experimental group with different dose and time. Agno 3 hair cells were stained with ABR threshold in 8 rats in each group. The effects of cisplatin on the morphology of cochlear hair cells and the expression of otoferlin protein in the inner hair cells of basal gyrus of basal membrane were detected by immunofluorescence reverse transcription-polymerase chain reaction (RT-PCR). After 4 days of administration, the ABR threshold of 1.5 mg / kg and 3.0mg/kg group was significantly higher than that of the normal group, and the ABR threshold of each experimental group on the 7th day was significantly higher than that of the normal group, and the threshold value of 0.75 mg / kg 脳 7 d group was significantly higher than that of the 0.75mg/kg 脳 4 d group at the same dose. There was no significant difference in the other doses. The expression of protein in the 3.0mg/kg group was significantly lower than that in the control group. There was no significant difference between the experimental group and the normal group. The morphological changes of hair cells in the group of 3.0 mg / kg 脳 7 d were the most obvious. Conclusion: low dose cisplatin can cause auditory impairment, and its mechanism may be related to the damage of inner hair cells and otoferlin protein.
【作者单位】：第三军医大学附属西南医院耳鼻咽喉-头颈外科;
【基金】：国家自然科学基金资助项目(No:30973301;81271080) 重庆市自然基金资助项目(No:CSTC2009BB5170)
【分类号】：R764

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