鼻咽癌组织中EBERs与BMI-1表达相关性研究

发布时间：2018-03-21 17:08

本文选题：鼻咽癌　切入点：EBERs　出处：《遵义医学院》2011年硕士论文　论文类型：学位论文

【摘要】：目的：探讨鼻咽癌组织中EBERs与BMI-1表达相关性。方法：对2007年12月至2009年11月收治的40例鼻咽癌患者的石蜡包埋切片进行研究。原位杂交方法检测这些切片中EBERs的表达。免疫组化方法检测这些切片中BMI-]的表达。结果：40例鼻咽癌组织中EBERs情况：(+)组14例、(++)组15例、(+++)组7例、(++++)组4例；合计低表达组为29例,高表达组为11例,阳性表达率为100%(40/40)。40例鼻咽癌组织中BMI-1情况：BMI-1表达阴性组27例, (+)组2例, (++)组8例, (+++)组3例,阳性表达率为32.5%(13/40)。鼻咽癌组织中EBERs表达与BMI-1表达差异有统计学意义,p值0.005,rs0.058。鼻咽癌组织中EBERs表达分组与BMI-1强阳性表达差异无统计学意义,p值=0.364。结论：检测鼻咽癌中EBERs表达,可以作为检测EBV感染的一种方法,至少在低分化鳞癌中是一种高敏感性、高特异性的方法。鼻咽癌中EBERs表达与BMI-1表达可能有正相关性,可以进一步研究证实,并探讨二者间的机理。
[Abstract]:Objective: to investigate the correlation between EBERs and BMI-1 expression in nasopharyngeal carcinoma (NPC). Methods: the paraffin embedded sections of 40 patients with nasopharyngeal carcinoma from December 2007 to November 2009 were studied. The expression of EBERs in these sections was detected by in situ hybridization and the expression of BMI-] was detected by immunohistochemistry. Results of the 40 cases of nasopharyngeal carcinoma, EBERs was found in 14 cases (15 cases), 15 cases (15 cases), 7 cases (4 cases), 29 cases in low expression group and 11 cases in high expression group. The positive expression rate was 100% 40% 40 cases of nasopharyngeal carcinoma (NPC), 27 cases of negative BMI-1 expression group, 2 cases of group (2), 8 cases of group (8), 3 cases of group (3). The positive expression rate was 32.5 / 40%. There was significant difference between EBERs expression and BMI-1 expression in nasopharyngeal carcinoma (P = 0.005 rs0.058). There was no significant difference between EBERs expression group and BMI-1 strong positive expression in nasopharyngeal carcinoma tissue (P = 0.364). Conclusion: detection of EBERs expression in nasopharyngeal carcinoma can be used as a method to detect EBV infection, at least it is a highly sensitive and specific method in poorly differentiated squamous cell carcinoma. There may be a positive correlation between EBERs expression and BMI-1 expression in nasopharyngeal carcinoma. The mechanism between the two can be further studied and verified.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.63

【参考文献】