豚鼠内耳前庭结构三维重建与温度场数值模拟的研究

发布时间：2018-03-23 17:14

本文选题：前庭　切入点：石蜡连续切片　出处：《大连医科大学》2011年硕士论文

【摘要】：目的: 1.本实验通过组织石蜡连续切片,结合计算机技术得到豚鼠内耳前庭膜迷路的三维几何模型,可清晰显示内耳前庭器官:膜半规管、椭圆囊及球囊等的形态结构,加深临床医生对内耳前庭宏细观结构的认识和准确量化理解,为建立异常条件下的动物实验模型及应用于人体实验奠定基础。 2.熟练掌握石蜡连续切片技术及计算机三维重建的方法,为内耳前庭各结构参数的精确测量和评价,以及生物力学数值模型的建立提供有效的工具。 3.对豚鼠内耳前庭三维重建模型进行温度场的数值模拟,分析温度刺激下前庭系统膜迷路内的生物力学响应。结合临床双温试验,有助于定量了解前庭系统维持平衡功能的机理,为前庭性疾病的诊疗提供一定的参考依据。方法:采用新鲜豚鼠颞骨,经过固定、脱钙、脱水、透明、浸蜡、包埋定位、切片机连续切片、染色、封片等,获得了片厚6μm的石蜡连续切片一套,采用数码摄像机进行采集,获取横断面二维图像数字信息,利用计算机技术进行三维重建,建立包含膜半规管、椭圆囊、球囊等细微结构的内耳前庭三维模型。基于三维成像模型进行数据测量,运用有限元方法进行内耳前庭温度场的数值模拟。结果: 1.获得了一套完整的豚鼠内耳组织连续切片及放大后图像资料。证实了从豚鼠内耳取材至切片、封片的实验步骤均具有可行性。 2.利用石蜡连续切片方法结合计算机技术,建立了豚鼠内耳前庭膜性结构的三维模型,可清楚的显示豚鼠内耳前庭的细微结构及空间特性,并得到了内耳前庭的三维数据。 3.对豚鼠内耳前庭三维成像模型进行温度场的数值模拟,显示了在温度刺激下前庭系统内复杂的力学行为。本文中给出了△T=+1℃、△T=+7℃及△T=-1℃三种温度刺激,观察随着时间的推移引起半规管壶腹嵴嵴顶的位移、速度及压强的变化。温度升高与降低时,位移均逐渐增大,速度均逐渐减小;温度升高,压强逐渐增大,温度降低,压强逐渐减小,以上指标最终均趋于一个稳定值。温差越大,力学响应越大。观察三个半规管同一温度刺激下嵴顶的位移响应得出:外半规管壶腹嵴嵴顶的位移响应最大,两垂直半规管壶腹嵴嵴顶的位移响应相对较小,且后半规管壶腹嵴嵴顶的位移响应是最小的。压强云图上可见压强降主要集中在外半规管。说明温度干预对外半规管影响较大。结论: 1.利用组织连续切片技术获取二维图像数字信息并进行计算机豚鼠内耳前庭三维重建,既具备了切片的细节性,又能反映其空间特性,而且可以进行三维数据测量,是一种提高颞骨形态学研究水平的方法,为今后的形态学教学及研究奠定了基础。 2.通过对内耳前庭温度场的数值模拟分析,能够定量了解前庭系统维持平衡的机理,为评价半规管及整个前庭系统的功能状态提供了一定的数据参考。 3.采用石蜡连续切片方法结合计算机技术用于内耳前庭宏细观结构三维重建并进行数值模拟的方法具有可行性。
[Abstract]:Objective:
1. this experiment through the paraffin serial sections, combined with computer technology the three-dimensional geometric model of guinea pig vestibular membrane lost, can clearly show the vestibular organs: the morphology and structure of membrane canal, utricular and saccular etc., deepen clinicians in the vestibular macro meso structure understanding and accurate quantitative understanding, to establish an animal model of abnormal under the condition of the human experiment and application to lay the foundation.
2., we have a good command of paraffin continuous slice technology and computer three-dimensional reconstruction method, providing an effective tool for the accurate measurement and evaluation of the structural parameters of the inner ear vestibule, and the establishment of a biomechanical numerical model.
Numerical simulation of temperature field of 3. in guinea pig vestibular 3D model, biomechanical analysis of temperature under the stimulation of the vestibular system film lost in response. Combined with clinical trials of double temperature mechanism contributes to quantitatively understand the balance function of the vestibular system, and to provide reference for diagnosis and treatment of vestibular disease.
Methods: the fresh guinea pig bone, after fixed, decalcified, dehydrated, transparent, paraffin embedding, serial sectioning, positioning, slicing machine staining, mounting, the thickness of 6 m paraffin serial sections of a digital camera, the acquisition, acquisition of digital cross sectional two-dimensional image information reconstructed using computer technology includes the establishment of the utricle, membranous semicircular canal, vestibular 3D fine structure. The balloon model based on 3D imaging measurement data, using the finite element method to simulate the temperature field of the vestibule.
Result:
1. get a complete set of inner ear serialsections and enlarge image data. Confirmed the story from the inner ear to the mounting section, experimental steps are feasible.
2., a three-dimensional model of the inner ear vestibule membrane structure of guinea pig was established by paraffin continuous slice method combined with computer technology. It can clearly show the fine structure and spatial characteristics of the inner ear vestibule of the guinea pig, and get the three-dimensional data of the inner ear vestibule.
Numerical simulation of temperature field of 3. in vestibule of guinea pig 3D imaging model, shows the complicated mechanical behavior under temperature stimulation in the vestibular system. This paper gives a T=+1 C, C and C delta T=+7 Delta T=-1 three temperature stimulation, observed with the passage of time caused by displacement of the semicircular canal crista crest, change the speed and pressure. The temperature rises and decreases, the displacement increase gradually, the speed decreases gradually; temperature increases, the pressure increases, the decrease of temperature, pressure decreases gradually, the above indexes are finally reached a stable value. The higher the temperature is, the greater the mechanical response. The observation that the same temperature stimulation under the crest displacement the response of the three semicircular canals: lateral displacement of the crista crest response maximum displacement, two vertical semicircular canal crista crest response is relatively small, and the displacement of the semicircular canal crista crest response pressure is minimal. The pressure drop is mainly concentrated in the outer semicircular canals, indicating that temperature intervention has a great influence on the external semicircular canals.
Conclusion:
1. use the serialsections technique to obtain two-dimensional image information and computer three-dimensional reconstruction of vestibule of guinea pig, not only has the details of the slice, and can reflect the space characteristics, but also can be used for 3D data measurement, is a method to improve the morphology of the temporal method of water level, which laid the foundation for the future research and teaching.
2., through the numerical simulation analysis of the vestibular temperature field in the inner ear, we can understand quantitatively the mechanism of maintaining the balance of the vestibular system, and provide some data reference for evaluating the functional state of semicircular canal and the whole vestibular system.
3. the method of continuous paraffin section combined with computer technology for three-dimensional reconstruction and numerical simulation of the macro mesoscopic structure of the vestibule of the inner ear is feasible.

【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R764

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