新生豚鼠高胆红素耳蜗核谷氨酸、γ-氨基丁酸含量表达

发布时间：2018-03-26 11:00

本文选题：新生豚鼠　切入点：高胆红素　出处：《中华耳科学杂志》2014年04期

【摘要】：目的观察高胆红素模型新生豚鼠耳蜗核谷氨酸(glutamicacid,Glu)和γ-氨基丁酸(gamma-aminobutyricacid,GABA)含量的变化,探讨Glu和GABA在高胆红素损伤模型低位听觉中枢耳蜗核中的作用和意义。方法选择正常新生豚鼠30只随机分为三组:对照组、低胆红素(100μg/g)处理组和高胆红素(200μg/g)处理组,8h后处死动物取材处理标本免疫荧光观察各组耳蜗核Glu和GABA含量,Western blotting(线粒体凋亡相关蛋白检测)观察细胞质细胞色素C含量变化。结果与对照组相比,高浓度和低浓度胆红素处理组耳蜗核中GABA的含量水平明显下降,差异有显著性(p0.05),而Glu的含量水平则明显上升(p0.01);Western blotting显示细胞色素C在胆红素高低浓度处理组表达明显升高。结论新生豚鼠高胆红素模型Glu和GABA含量的动态变化反映了神经元的活动状况,提示二者在胆红素耳神经毒性中可能起重要作用。细胞质细胞色素C增高说明高胆红素可能介导神经细胞早期凋亡。
[Abstract]:Objective to observe the changes of glutamic acid glutamate (glutamic acid) and gamma-aminobutyric acid (GABA) in the cochlear nucleus of neonatal guinea pigs with hyperbilirubin model. To investigate the role and significance of Glu and GABA in the central cochlear nucleus of low hearing in the model of hyperbilirubin injury, 30 normal newborn guinea pigs were randomly divided into three groups: control group. Low bilirubin 100 渭 g / g and hyperbilirubin 200 渭 g / g) treatment group for 8 h, then killed the animals to observe the content of Glu and GABA in the cochlear nucleus and the content of cytochrome C in the cytoplasm of each group. The results were compared with those in the control group. The content of GABA in cochlear nucleus of high and low concentration bilirubin group decreased obviously. There was a significant difference between the two groups, and the level of Glu was significantly increased. Western blotting showed that the expression of cytochrome C in bilirubin treatment group was significantly increased. Conclusion the dynamic changes of Glu and GABA levels in neonatal guinea pig model of hyperbilirubin are reflected. The activity of neurons, These results suggest that both of them may play an important role in the ototoxicity of bilirubin. The increase of cytochrome C in cytoplasm suggests that hyperbilirubin may mediate early apoptosis of nerve cells.
【作者单位】：南方医科大学南方医院耳鼻咽喉头颈外科;广东药学院附属第二医院耳鼻咽喉头颈外科;
【分类号】：R764

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