激光扫描共聚焦显微镜观测大黄素β-环糊精包合物在鼻咽癌细胞的跨膜转运及分布

发布时间：2018-03-26 20:14

本文选题：医学光学　切入点：药物跨膜转运　出处：《中国激光》2014年05期

【摘要】：利用激光扫描共聚焦显微镜动态研究大黄素β-环糊精包合物在鼻咽癌CNE-1细胞的跨膜转运及分布。结果显示,大黄素β-环糊精包合物以颗粒的形式主要分布于胞浆中,在其浓度为5~40mg·L-1时细胞对其摄取量随浓度的增加呈非线性增加;NaN3、甘露醇可抑制CNE-1细胞对大黄素β-环糊精包合物的摄入量,环孢菌素A(CsA)在药物浓度低(小于5mg·L-1)时可显著增加细胞对大黄素β-环糊精包合物的摄入量,而在药物浓度大时,反而抑制细胞的摄取量。相对大黄素而言,大黄素β-环糊精包合物在细胞内持续时间较长。大黄素β环糊精包合物在鼻咽癌CNE-1细胞的跨膜转运主要遵循能量依赖的内吞模式且受P-gp蛋白的调控,这一特性可为药物剂型研究提供参考。
[Abstract]:The transmembrane transport and distribution of emodin 尾 -cyclodextrin inclusion complex in nasopharyngeal carcinoma (NPC) CNE-1 cells were studied by laser scanning confocal microscopy. The results showed that emodin 尾 -cyclodextrin inclusion complex mainly distributed in the cytoplasm in the form of granules. When the concentration was 5~40mg L-1, the uptake of NAN3 was increased linearly with the increase of concentration. Mannitol inhibited the intake of emodin 尾 -cyclodextrin inclusion compound by CNE-1 cells. Cyclosporin A (CsA) significantly increased the intake of emodin 尾 -cyclodextrin inclusion complex when the drug concentration was lower than that of 5mg L-1, but inhibited the uptake of emodin 尾 -cyclodextrin when the drug concentration was high. The transmembrane transport of emodin 尾 -cyclodextrin inclusion complex in nasopharyngeal carcinoma (NPC) CNE-1 cells mainly follows an energy-dependent endocytosis pattern and is regulated by P-gp protein. This characteristic can be used as a reference for the study of drug formulation.
【作者单位】：广西医科大学药学院;广西医科大学医学科学实验中心;
【基金】：国家自然科学基金(81060270) 广西医学科学实验中心开放基金(KFJJ2011-25)
【分类号】：R739.63

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