不同磁场强度的硅胶包埋磁片对缺血性眼病的影响

发布时间：2018-03-29 23:23

本文选题：磁场　切入点：缺血性眼病　出处：《延边大学》2011年硕士论文

【摘要】：目的：观察不同磁场强度的硅胶包埋磁片对缺血性眼病的影响,探讨磁场对缺血性眼病治疗作用与机制。方法：80只家兔随机分5组n=16,阴性对照组,200GS组,400GS组,800GS组,阳性对照组。左眼制作视网膜动脉缺血后,分别用200GS、400GS、800GS磁片巩膜后埋藏,对照组埋藏硅胶片。给予每组家兔实验开始时和半年时测量视网膜电流图(ERG)和眼动电流图(EOG)。每组随机取四只家兔一周时行荧光素眼底血管造影(Fundus fluorescein angiography, FFA)检查,半个月时再次行FFA检查,一个月时最后行FFA检查。另外四只在一个月时做血清学检查后处死,取缺血部位眼组织做组织学检查。结果200GS、400GS、800GS组及阳性对照组均有不同程度的缺血改善,阴性对照组无改善。结果：动物实验结果显示：与对照组比较,200GS组、400GS组、800GS组、其ERG波形明显改变,为负反应,低于正常,不肯定,正常,尤其以800GS组的波形正常。并且其FFA充盈时间明显缩短。组织学和抗氧化指标检测结果显示：与对照组比较,各组血清中SOD、GSH-PX的活力增高,MDA含量则明显下降,且与磁场成正比关系；另外与对照比较,各组视网膜组织中双极细胞层凋亡明显减少,800GS组尤其明显。结论：硅胶包膜巩膜后埋藏磁片通过其抗氧化作用有效地改善缺血性眼病症状。
[Abstract]:Aim: to investigate the effect of magnetic field on ischemic ophthalmopathy and explore the mechanism of magnetic field on ischemic ophthalmopathy. Methods Twenty eight rabbits were randomly divided into 5 groups (n = 16), negative control group (n = 200GS) and positive control group (n = 800GS). The control group was implanted with silica gel tablets. The retinal electroretinogram (ERG) and EOG were measured at the beginning of the experiment and half a year in each group. Four rabbits in each group were randomly selected for fundus fluorescein angiographic (FFAA) examination at one week. Half a month after another FFA examination, one month at the end of the FFA test. The other four were executed after a serological examination at one month. Results there were different degrees of ischemic improvement in 200GSN 400GS800GS group and positive control group, but there was no improvement in negative control group. Results: compared with the control group, the ERG waveform of the 200GS group was obviously changed, which was negative, lower than that of the normal, uncertain and normal. Especially, the waveform of 800GS group was normal, and the filling time of FFA was shortened obviously. The results of histological and antioxidant indexes showed that the activity of SOD- GSH-PX in serum of each group was significantly lower than that of control group, which was proportional to the magnetic field. In addition, compared with the control group, the apoptosis of bipolar cell layer in the retina of each group was significantly reduced. Conclusion: silicone gel coated sclera embedded magnetic disc can effectively improve the symptoms of ischemic ophthalmopathy through its antioxidation effect.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R77

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