消旋山莨菪碱对豚鼠形觉剥夺性近视的影响及机制研究

发布时间：2018-03-31 02:29

本文选题：消旋山莨菪碱　切入点：豚鼠　出处：《复旦大学》2011年硕士论文

【摘要】：第一部分10%消旋山莨菪碱对豚鼠形觉剥夺性近视的作用和安全性目的：探讨10%消旋山莨菪碱对豚鼠形觉剥夺性近视的作用以及安全性。方法：2-3周龄英国短毛花色豚鼠30只,随机分为3组,每组10只,采用右眼遮盖半透膜建造形觉剥夺模型,左眼为自身对照,A组形觉剥夺+10%消旋山莨菪碱20μl玻璃体腔注射1次,B组形觉剥夺+生理盐水20μl玻璃体腔注射1次,C组单纯形觉剥夺组。所有动物饲养于5001ux白光照明环境中,照明周期为12h：12h。干预前后用A超测量眼轴、前房深度和晶体厚度,算出玻璃体腔长度；带状光检影法测量屈光度；曲率计测量角膜曲率半径；右眼与左眼的差异作为评价指标以消除饲养过程中的组间差异。2周后实验结束每组随机选取2只豚鼠处死后立即摘取眼球,行HE染色观察眼部组织结构。SPSS软件行统计分析,以P0.05为统计学差异。结果：1、各组基线测量左右眼眼轴、前房深度、晶体厚度、玻璃体腔长度、屈光度、角膜曲率均无统计学差异(P0.05)。2、干预后2周复测,给药组左右眼眼轴和玻璃体腔差异明显小于对照组(P0.05),玻璃体腔长度差异与眼轴长度差异显著相关(r=0.904,P0.001)。各组左右眼屈光度差异、曲率半径差异、晶体厚度差异、前房深度差异无统计学意义(P0.05)。3、HE染色后显微镜下观察给药组角膜、睫状体、视网膜、巩膜均未见毒性改变。结论：10%消旋山莨菪碱可抑制豚鼠形觉剥夺性近视的眼轴增长,主要抑制玻璃体腔长度。10%消旋山莨菪碱玻腔注射未发现组织学毒性改变。目的：探讨10%消旋山莨菪碱玻腔注射对豚鼠形觉剥夺性近视眼球后极部巩膜TGF-β2和MMP-2蛋白表达的影响。方法：2-3周龄英国短毛花色豚鼠18只,随机分为3组,每组6只,采用右眼遮盖半透膜建造形觉剥夺模型,左眼为自身对照,A组形觉剥夺+10%消旋山莨菪碱20μl玻璃体腔注射1次,B组单纯形觉剥夺组,C组正常空白对照组。所有动物饲养于5001ux白光照明环境中,照明周期为12h：12h。2周后处死豚鼠取右眼后极部巩膜组织,采用蛋白印迹法检测MMP-2蛋白和TGF-β2蛋白含量。结果：巩膜MMP-2蛋白表达从高到低依次为：形觉剥夺组,10%消旋山莨菪碱组,正常对照组。巩膜TGF-β2表达从从高到低依次为：正常对照组,10%消旋山莨菪碱组,形觉剥夺组。结论：10%消旋山莨菪碱玻腔注射可使形觉剥夺后豚鼠巩膜蛋白表达发生变化；10%消旋山莨菪碱抑制MMP-2蛋白表达,调高TGF-β2蛋白表达；这一作用是否参与干预巩膜重塑过程,并对近视进展产生影响,值得进一步研究。
[Abstract]:Effect and safety of 10% racemic anisodamine on form-deprivation myopia in guinea pigsObjective: to investigate the effect and safety of 10% racemic anisodamine on form-deprivation myopia in guinea pigs.Methods Thirty British short-haired pigmented guinea pigs aged 2-3 weeks were randomly divided into 3 groups with 10 guinea pigs in each group. The model of shape deprivation was constructed by covering the right eye with semi-permeable membrane.The left eye in group A was deprived of 10% racemic anisodamine 20 渭 l intravitreal injection. Group B was injected with normal saline 20 渭 l once in group C and group C was deprived of simple form perception.All the animals were raised in 5001ux white lighting environment, and the illumination period was 12 h: 12 h.Before and after intervention, the length of vitreous cavity was calculated by measuring the depth of eye axis, anterior chamber depth and lens thickness by A-ultrasound, the diopter was measured by zonal photogrammetry, the radius of curvature of cornea was measured by curvature meter.The difference between right eye and left eye was used as evaluation index to eliminate the difference between groups during feeding. 2 weeks later, two guinea pigs in each group were randomly selected to take out the eyeball immediately after death, and the eye tissue structure was observed by HE staining. SPSS software was used for statistical analysis.P0.05 as the statistical difference.Results: there was no significant difference in eye axis, anterior chamber depth, lens thickness, length of vitreous cavity, diopter and corneal curvature in all groups.The difference of eye axis and vitreous cavity in the treatment group was significantly lower than that in the control group (P 0.05), and there was a significant correlation between the length of the vitreous cavity and the length of the eye axis.The difference of diopter, radius of curvature, thickness of lens and depth of anterior chamber were not statistically significant in each group. No toxic changes were found in cornea, ciliary body, retina and sclera after HE staining.Conclusion 10% racemic anisodamine can inhibit the axial growth of form-deprived myopia in guinea pigs, and no histological toxicity was found after vitreous injection of 10% racemic anisodamine.Aim: to investigate the effect of 10% racemic anisodamine injection on the expression of TGF- 尾 2 and MMP-2 protein in posterior sclera of form-deprived myopia in guinea pigs.Methods Eighteen British short-haired pigmented guinea pigs aged 2-3 weeks were randomly divided into 3 groups (6 in each group). The model of shape deprivation was constructed by covering the right eye with semi-permeable membrane.The left eye was given 10% racemic anisodamine 20 渭 l intravitreal injection into the vitreous cavity of the control group A (n = 10). Group B (n = 10) was injected once into the vitreous cavity of group B, and group C (n = 10) was normal blank control group.All the animals were raised in the white light environment of 5001ux. After 12h:12h.2 week, the guinea pigs were killed to take the sclera tissue of the posterior pole of the right eye. The contents of MMP-2 protein and TGF- 尾 2 protein were detected by Western blotting.Results: the expression of MMP-2 protein in sclera from high to low was 10% racemic anisodamine group and normal control group.The order of TGF- 尾 2 expression in sclera from high to low was: normal control group, 10% racemic anisodamine group, form deprivation group.Conclusion injection of 10% racemic anisodamine into vitreous cavity can change the scleral protein expression of guinea pigs after form deprivation. 10% racemic anisodamine can inhibit the expression of MMP-2 protein and increase the expression of TGF- 尾 2 protein, and whether this effect is involved in the process of scleral remodeling.It has an effect on myopia progression and is worthy of further study.
【学位授予单位】：复旦大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R778.11

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