微电流检测和EBER原位杂交联合应用在鼻咽癌病理诊断中的意义

发布时间：2018-04-11 22:26

本文选题：鼻咽癌 + EBER/原位杂交　；参考：《中国现代医学杂志》2014年29期

【摘要】：目的研究微电流值在鼻咽癌组织中的变化特点及EBER在鼻咽癌的表达情况。探索微电流检测和EBER原位杂交联合应用在鼻咽癌病理诊断中的意义。方法应用微电流检测和EBER分子原位杂交方法检测48例鼻咽癌、19例鼻咽黏膜慢性炎症,分析微电流值在鼻咽癌组织中的变化特点及EBER在鼻咽癌的表达情况。结果微电流检测的48例鼻咽癌中40例阳性(微电流值0μA),阳性率为83.3%,19例鼻咽黏膜慢性炎症组织中3例阳性(微电流值0μA),假阳性率15.8%;EBER原位杂交检测的48例鼻咽癌中38例呈阳性反应,阳性率为79.2%,19例鼻咽黏膜慢性炎症组织均阴性;微电流检测和EBER原位杂交联合检测48例鼻咽癌中44例阳性,阳性率为91.7%,差异有统计学意义。结论 1微电流检测和EBER原位杂交检测方法是较好的鼻咽癌病理诊断方法。2微电流检测和EBER原位杂交在鼻咽癌病理诊断中有相互补充作用,联合应用两种方法,可以提高鼻咽癌诊断正确率,减少假阴性率。
[Abstract]:Objective to study the changes of microcurrent in nasopharyngeal carcinoma (NPC) and the expression of EBER in nasopharyngeal carcinoma (NPC).To explore the significance of microcurrent detection and EBER in situ hybridization in the pathological diagnosis of nasopharyngeal carcinoma.Methods the microcurrent and EBER molecular in situ hybridization were used to detect the chronic inflammation of nasopharyngeal mucosa in 48 patients with nasopharyngeal carcinoma. The changes of microcurrent in nasopharyngeal carcinoma and the expression of EBER in nasopharyngeal carcinoma were analyzed.Results among 48 cases of nasopharyngeal carcinoma detected by microcurrent, 40 cases were positive (microcurrent value 0 渭 Ag), the positive rate was 83.3% and 3 out of 19 cases of chronic inflammation of nasopharyngeal mucosa (microcurrent value 0 渭 AN), the false positive rate was 15.8% EBER in 48 cases of nasopharyngeal carcinoma, 38 cases showed positive reaction.The positive rate was 79.2% in 19 cases of nasopharyngeal mucosa chronic inflammation and 44 cases (91.7%) were positive in 48 cases of nasopharyngeal carcinoma by microcurrent detection and EBER in situ hybridization. The difference was statistically significant.Conclusion 1 Micro-current detection and EBER in situ hybridization are better methods for the pathological diagnosis of nasopharyngeal carcinoma. 2. The microcurrent detection and EBER in situ hybridization are complementary to each other in the pathological diagnosis of nasopharyngeal carcinoma.It can improve the correct diagnosis rate of nasopharyngeal carcinoma and reduce false negative rate.
【作者单位】：广西中医药大学附属瑞康医院病理科;广西中医药大学第一附属医院病理科;
【基金】：广西科学研究与技术开发计划项目(No:桂科攻1140003B-54)
【分类号】：R739.63

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