IL-18对分泌性中耳炎模型大鼠中耳IFN-γ和IL-4表达的影响

发布时间：2018-04-13 18:55

本文选题：白细胞介素 + 分泌性中耳炎　；参考：《听力学及言语疾病杂志》2014年01期

【摘要】：目的探讨白细胞介素18(interleukin 18,IL-18)对分泌性中耳炎(otitis media with effusion,OME)模型大鼠中耳炎症细胞的增殖情况以及干扰素γ(interferonγ,IFN-γ)和白细胞介素4(interleukin 4,IL-4)表达的影响。方法 18只SD大鼠随机分为对照组(A组)、OME模型组(B组)和IL-18注射组(C组),每组各6只(12耳),B组和C组采用卵清蛋白(ovalbumin,OVA)腹腔注射致敏后耳内激发制成OME模型,C组于OVA全身致敏及耳内激发的同时,在实验第1、2、7、8、15、16d进行腹腔注射IL-18 1μg加生理盐水0.2ml,A组和B组在相同时间点腹腔注射生理盐水0.2ml。HE染色观察各组中耳黏膜病理变化及炎症细胞的改变,ELISA法测定各组大鼠中耳腔灌洗液中T辅助细胞(T helper cell,Th)分泌Th2型细胞因子-IL-4和Th1型细胞因子-IFN-γ的含量。结果 C组大鼠中耳黏膜组织炎症反应较B组无明显减轻,中耳黏膜下层和骨髓腔中中性粒细胞数量较B组显著增多(P0.05),嗜酸粒细胞数量与B组比较稍减少(P0.05),但仍明显多于A组(P0.05);C组中耳灌洗液Th1型细胞因子IFN-γ含量明显高于B组与A组(P0.05),Th2型细胞因子IL-4含量较B组稍有增高(P0.05);但B组和C组IL-4含量均明显高于A组(P0.05);A、C组Th1/Th2比值虽高于B组,但差异无统计学意义(P0.05)。结论 IL-18诱导OME模型大鼠中耳微环境中IFN-γ合成显著增高,虽然一定程度上缓解了OME大鼠中耳Th1/Th2失衡状态,但是仍存在以Th细胞过度活化、Th1和Th2型细胞因子过度分泌的免疫反应紊乱状态。
[Abstract]:Objective to investigate the effects of interleukin 18(interleukin 18 (IL 18) on the proliferation of middle ear inflammatory cells and the expression of Interferon 纬 interferon 纬 (IFN- 纬) and interleukin 4(interleukin 4 (IL 4) in rats with secretory otitis media media with effusion.Methods Eighteen Sprague-Dawley rats were randomly divided into two groups: control group (group A) and control group (group B) and IL-18 injection group (group C). Six rats in each group were sensitized with ovalbumin ovalbumin ovalbumin.OVA was sensitized throughout the body and stimulated in the ear at the same time,Intraperitoneal injection of IL-18 1 渭 g plus normal saline 0.2 ml 0.2ml.HE staining was performed at the first day of the experiment for 16 days to observe the pathological changes of middle ear mucosa and the changes of inflammatory cells in the middle ear of rats in each group. Elisa method was used to determine the middle ear of rats in each group.The levels of Th2 type cytokines (-IL-4) and Th1 type cytokines (-IFN- 纬) were secreted by T helper cells (T helper) in the lavage fluid.Results the inflammatory reaction of middle ear mucosa in group C was less than that in group B.The number of neutrophils in submucosa and medullary cavity of middle ear was significantly higher than that in group B (P 0.05), and the number of eosinophil in group B was slightly lower than that in group B (P 0.05), but it was still significantly higher than that in group A (group A) and group C (P 0.05). The content of IFN- 纬 in the lavage fluid of middle ear was significantly higher than that in group B.The IL-4 content of Th2 type cytokines in group B and group A was slightly higher than that in group B, but the content of IL-4 in group B and group C was significantly higher than that in group A (P 0.05) and the ratio of Th1/Th2 in group A was higher than that in group B.But the difference was not statistically significant (P 0.05).Conclusion the synthesis of IFN- 纬 in the middle ear microenvironment of OME model rats induced by IL-18 was significantly increased, although to some extent alleviated the imbalance of Th1/Th2 in the middle ear of OME rats.However, there is still a disorder of immune response in which Th cells over-activate Th1 and Th2 type cytokines.
【作者单位】：河北医科大附属唐山市工人医院耳鼻喉科;首都医科大学附属北京同仁医院耳鼻咽喉头颈外科;北京市耳鼻咽喉科研究所基础部;首都医科大学附属北京复兴医院;
【分类号】：R764

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