CK19和EGFR mRNA在喉癌动物模型外周血中的表达及其与淋巴转移关系

发布时间：2018-04-17 04:20

本文选题：细胞角蛋白19 + 表皮生长因子受体　；参考：《重庆医科大学》2011年硕士论文

【摘要】：目的:探索外周血中的循环播散肿瘤细胞(circulation tumor cell,CTC)与喉癌淋巴结转移的相关性,评价CK19和EGFR在喉癌微转移诊断中的价值。方法:从40只裸鼠中随机选择35只为实验组,平均分为5组,每组7只,建立喉癌裸鼠成瘤模型,其余5只为空白对照组。于接种后2、4、6、8、10周分别处死1组,采用RT-PCR方法检测裸鼠外周血及肿瘤组织中CK19 mRNA和EGFR mRNA表达,采用连续病理切片HE染色法观察淋巴结转移情况,免疫组化SP法检测肿瘤组织中CK19和EGFR蛋白的表达。结果:裸鼠成瘤模型构建成功。喉癌裸鼠动物模型中有20只发生淋巴结转移,转移率为57.1%(20/35)。空白对照组外周血中未发现CK19 mRNA和EGFR mRNA表达,实验组外周血和肿瘤组织中CK19 mRNA阳性率分别为54.3%(19/35)和80%(28/35),EGFR mRNA的阳性率分别为37.1%(13/35)和74.3%(26/35),CK19和EGFR是CTC的特异性标记物,淋巴结转移与外周血CTC相关(P0.001),且多发生在肿瘤指数生长期。免疫组化检测结果发现CK19和EGFR蛋白在肿瘤组织中阳性率分别为80%(28/35)和85.7%(30/35)。结论:外周血中CK19和EGFR的表达有助于预测淋巴结转移,而CK19和EGFR是外周血循环播散肿瘤细胞的特异性标记物,提示CTC与喉癌淋巴转移具有相关性。
[Abstract]:Objective: to investigate the relationship between circulating tumor cell CTCs and lymph node metastasis in laryngeal carcinoma, and to evaluate the value of CK19 and EGFR in the diagnosis of micrometastasis of laryngeal carcinoma.Methods: 35 nude mice were randomly divided into 5 groups, 7 in each group, to establish tumorigenic model of laryngeal carcinoma, and the other 5 were blank control group.The expression of CK19 mRNA and EGFR mRNA in peripheral blood and tumor tissues of nude mice were detected by RT-PCR method, and lymph node metastasis was observed by HE staining in serial sections.Immunohistochemical SP method was used to detect the expression of CK19 and EGFR in tumor tissues.Results: the tumorigenic model of nude mice was successfully constructed.Lymph node metastasis occurred in 20 nude mice with laryngeal carcinoma, and the metastasis rate was 57.1%.No expression of CK19 mRNA and EGFR mRNA was found in the peripheral blood of the blank control group. The positive rates of CK19 mRNA in peripheral blood and tumor tissues in the experimental group were 54.3 / 35 and 80 / 28 / 35, respectively. The positive rates of CK19 mRNA in peripheral blood of the control group were 37.1T / 35) and 74.33.33% / 35% / CK19 and EGFR were specific markers of CTC, respectively.Lymph node metastasis was associated with peripheral blood CTC (P 0.001), and most of them occurred in the growth stage of tumor index.Immunohistochemical results showed that the positive rates of CK19 and EGFR in tumor tissues were 80 / 28 / 35) and 85.730 / 35 / 35 respectively.Conclusion: the expression of CK19 and EGFR in peripheral blood is helpful to predict lymph node metastasis, while CK19 and EGFR are specific markers for circulating tumor cells in peripheral blood, suggesting that CTC is associated with lymphatic metastasis of laryngeal carcinoma.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.65

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