塞来昔布与顺铂联合应用抑制顺铂耐药性鼻咽癌细胞的生长并诱导其凋亡

发布时间：2018-04-18 19:50

本文选题：塞来昔布 + 顺铂　；参考：《细胞与分子免疫学杂志》2015年02期

【摘要】：目的观察塞来昔布(celecoxib)与顺铂(DDP)联合处理对DDP耐药的鼻咽癌TW03/DDP细胞生长的影响。方法用(0.05~1.00)μg/m L的DDP梯度培养TW03/DDP细胞,经反转录PCR和Western blot法证明TW03/DDP细胞肺耐药相关蛋白(LRP)高表达后,采用siRNA干扰TW03/DDP细胞LRP蛋白表达;Celecoxib与DDP联合或单独处理不具耐药性的鼻咽癌TW03细胞、TW03/DDP细胞和TW03/DDP-siRNA细胞。CCK-8法检测细胞的生长抑制率,annexin V-FITC/碘化丙锭(PI)双染色结合流式细胞术检测细胞凋亡率。结果 Celecoxib与DDP单独使用均能抑制TW03/DDP细胞和TW03细胞的生长并诱导凋亡,但DDP对TW03/DDP细胞作用弱;Celecoxib与DDP联用对2个细胞株的生长都有抑制作用,其生长抑制率和凋亡率均大于二者单独应用的效果;Celecoxib处理TW03/DDP细胞可使其LRP的mRNA和蛋白水平下降。结论 Celecoxib和DDP联合应用可抑制LRP的表达,显著抑制TW03/DDP细胞的生长并诱导其凋亡。
[Abstract]:Objective To observe the effect of celecoxib (celecoxib) and cisplatin (DDP) combined treatment effect on the growth of DDP resistant human nasopharyngeal carcinoma cell line TW03/DDP. Methods using (0.05~1.00) DDP g/m L gradient cultured TW03/DDP cells by reverse transcription of PCR and Western blot of TW03/DDP cell lung resistance by Guan Danbai (LRP) high expression after use siRNA interference of TW03/DDP cells LRP protein expression; Celecoxib combined with DDP treatment alone or with drug resistance of nasopharyngeal carcinoma TW03 cells, TW03/DDP cells and TW03/DDP-siRNA cells detected by.CCK-8 cell growth inhibition rate, annexin V-FITC/ propidium iodide (PI) staining and cell apoptosis rate were detected by flow cytometry. Results of Celecoxib and DDP used alone can inhibit the TW03/DDP cell and TW03 cell growth and induce apoptosis in TW03/DDP cells, but the effect of DDP on Celecoxib and DDP combined with weak; on the 2 cell growth was inhibited, the Growth inhibition rate and apoptosis rate were all greater than those of two application alone. Celecoxib treated TW03/DDP cells decreased the level of mRNA and protein of LRP. Conclusion combination of Celecoxib and DDP can inhibit LRP expression, significantly inhibit TW03/DDP cell growth and induce apoptosis.

【作者单位】：内江市第一人民医院医务科;四川大学华西医院耳鼻喉科;
【基金】：四川省科技厅科技支撑计划(2012SZZ011)
【分类号】：R739.63

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