核心蛋白多糖抑制翼状胬肉成纤维细胞增生的实验研究

发布时间：2018-04-18 19:47

本文选题：核心蛋白多糖 + 翼状胬肉　；参考：《眼科新进展》2014年08期

【摘要】：目的观察核心蛋白多糖对体外培养人翼状胬肉成纤维细胞(human pterygium fibroblasts,HPF)增生和凋亡的影响,寻找辅助治疗和预防翼状胬肉切除术后复发的新方法。方法采用组织块法体外培养HPF,将处于对数生长期的细胞用于实验。将0.1 mg·L-1、1.0 mg·L-1、10.0 mg·L-1的Decorin分别加入细胞培养基中培养24 h、48 h、72 h。MTT比色法分别测定不同浓度的Decorin作用于HPF不同时间后的细胞生长抑制率,流式细胞仪测定细胞周期时相变化,免疫组织化学法染色PCNA检测细胞生长活性。结果 MTT结果显示不同浓度组Decorin作用HPF 24 h细胞生长抑制率分别为(7.81±1.26)%、(19.52±2.14)%、(42.69±1.62)%,作用48 h分别为(10.19±2.74)%、(22.28±0.97)%、(45.73±0.82)%,作用72 h分别为(20.49±1.39)%、(29.50±2.47)%、(59.41±1.71)%。随着药物浓度的逐渐增高和作用时间的延长,细胞生长抑制率逐渐增加,差异均有统计学意义(均为P0.05)。流式细胞仪检测各浓度Decorin组HPF中G0/G1期细胞比率均较空白对照组高,差异均有统计学意义(均为P0.05)。当Decorin浓度在0.1~10.0 mg·L-1范围内作用HPF 72 h,均能够明显抑制细胞表达PCNA(均为P0.05)。结论 Decorin可以抑制HPF的增生,诱导其凋亡,且呈明显的剂量和时间依赖性,有望成为防治翼状胬肉的药物之一。
[Abstract]:Objective to observe the effect of core proteoglycan on proliferation and apoptosis of human pterygium fibroblasts in vitro, and to find a new method to treat and prevent the recurrence of pterygium after pterygium excision.Methods HPFs were cultured in vitro by tissue mass method, and the cells in logarithmic growth phase were used in the experiment.The cell growth inhibition rate of HPF treated with 0.1 mg L ~ (-1) and 1.0 mg ~ (-1) L ~ (-1) 10 mg 路L ~ (-1) Decorin for 24 h and 48 h ~ (72) h.MTT were determined by flow cytometry, and the cell cycle phase changes were measured by flow cytometry.Cell growth activity was detected by immunohistochemical staining PCNA.Results the results of MTT showed that the growth inhibition rates of HPF cells in different concentration groups were 7.81 卤1.26 and 19.52 卤2.14, 10.19 卤2.74, 22.28 卤0.97, and 20.49 卤1.39 for 72 h, respectively, and 29.50 卤2.47, 59.41 卤1.71, 59.41 卤1.71, respectively, in 48 hours.With the increase of drug concentration and the prolongation of the time of action, the inhibition rate of cell growth increased gradually, the difference was statistically significant (all P 0.05).The percentage of G0/G1 phase cells in HPF of Decorin group was higher than that of control group by flow cytometry, and the difference was statistically significant (P 0.05).When the concentration of Decorin was in the range of 0.1 ~ 10.0 mg ~ (-1) mg ~ (-1) for 72 h, the expression of PCNA was significantly inhibited (both P0.05 ~ (-1)).Conclusion Decorin can inhibit the proliferation of HPF and induce its apoptosis in a dose-and time-dependent manner, which may be one of the drugs for the prevention and treatment of pterygium.
【作者单位】：三峡大学第一临床医学院宜昌市中心人民医院眼科;
【分类号】：R777.33

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