氟伐他汀对大鼠角膜碱烧伤后新生血管形成的影响

发布时间：2018-04-21 22:43

本文选题：氟伐他汀 + 角膜　；参考：《武汉大学学报(医学版)》2014年04期

【摘要】：目的:探讨氟伐他汀对大鼠角膜碱烧伤后新生血管形成的作用及其机制。方法:30只SD大鼠随机分为3组:生理盐水组、氟伐他汀组和地塞米松组,每组10只,均用1mol/L的NaOH烧伤大鼠右眼,生理盐水组、氟伐他汀组和地塞米松组分别以生理盐水、10-5 mol/L氟伐他汀和1g/L地塞米松点眼。碱烧伤后1-14d用裂隙灯观察角膜的混浊度和新生血管的生长情况,HE染色观察角膜组织病理学改变,免疫组化观察VEGF和CD31的表达情况。结果:碱烧伤后第3,7,14天,氟伐他汀组和地塞米松组的角膜混浊度评分和新生血管生长评分都显著低于生理盐水组,差异有统计学意义,P0.05。组织病理学检查结果提示,与生理盐水组相比,氟伐他汀组和地塞米松组的角膜组织结构明显整齐,新生血管的形成明显减少,伴少量炎性细胞浸润。免疫组化结果显示氟伐他汀组和地塞米松组的VEGF和CD31的表达显著减少。结论:氟伐他汀能减轻大鼠角膜碱烧伤,其机制可能是下调VEGF和CD31的表达。
[Abstract]:Aim: to investigate the effect and mechanism of fluvastatin on neovascularization after alkali burn of cornea in rats. Methods Thirty Sprague-Dawley rats were randomly divided into three groups: normal saline group, fluvastatin group and dexamethasone group with 10 rats in each group. All rats were treated with 1mol/L in the right eye and normal saline group. The eyes of fluvastatin group and dexamethasone group were treated with normal saline for 10-5 mol/L fluvastatin and 1g/L dexamethasone respectively. The corneal turbidities and neovascularization were observed by slit lamp from 1 to 14 days after alkali burn. The histopathological changes of cornea were observed by HE staining and the expression of VEGF and CD31 were observed by immunohistochemistry. Results: the corneal turbidity score and neovascularization score in fluvastatin group and dexamethasone group were significantly lower than those in saline group (P 0.05). Histopathological examination showed that the corneal tissue structure of fluvastatin group and dexamethasone group was obviously neat compared with normal saline group. The formation of neovascularization was obviously decreased and a small amount of inflammatory cells infiltrated in fluvastatin group and dexamethasone group. Immunohistochemical results showed that the expression of VEGF and CD31 in fluvastatin group and dexamethasone group decreased significantly. Conclusion: fluvastatin can attenuate the alkali burn of cornea in rats by down-regulating the expression of VEGF and CD31.
【作者单位】：武汉大学人民医院;
【分类号】：R779.1

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