间歇低氧小鼠血清氧化应激指标的变化及N-乙酰半胱氨酸的治疗作用

发布时间：2018-04-25 18:58

本文选题：间歇低氧 + N-乙酰半胱氨酸　；参考：《苏州大学》2011年硕士论文

【摘要】：目的观察慢性间歇低氧((chronic intermittent hypoxia,CIH)小鼠血清氧化应激指标的变化情况,并初步探讨N-乙酰半胱氨酸(N-acetylcystein,NAC)的治疗作用。方法将50只小鼠随机分为正常对照组(unhandled control group, UC) 20只,CIH组30只,分别进行间歇低氧3天、1周、2周、3周、4周;另设CIH 4周+NAC组6只。分别采用硫代巴比妥酸法、羟胺法、化学比色法检测各组小鼠血清丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(Superoxide Dismutase,SOD)、和谷胱甘肽过氧化物(glutathione peroxidase,GSH-PX)表达水平,观察随着间歇低氧造模时间延长,各指标的变化情况,以及NAC的治疗作用。结果(1)在间歇低氧造模第3天时,CIH组小鼠血清MDA水平较对照组有所升高,较UC组比较差异有统计学意义,(CIH 8.98±0.50 nmol/mL UC 7.82±0.49 nmol/mL,p0.05)。随着间歇低氧时间的延长,CIH组小鼠血清MDA水平呈逐渐升高趋势,各造模时间段两组之间比较,差异均有统计学意义(p0.05或p0.01)。另外,在间歇低氧造模3天时,CIH组小鼠血清SOD、GSH-PX水平较对照组有所下降,但差异无统计学意义(p0.05);自低氧第1周始,CIH组小鼠血清SOD、GSH-PX分别为83.30±7.32U/mL、211.5±13.39 U/mL,较UC组95.14±8.4 U/mL、233.84.±15.32 U/mL比较差异有统计学意义(p0.05)。随着间歇低氧时间的延长,CIH组小鼠血清SOD、GSH-PX水平呈逐渐下降趋势,各造模时间段两组之间比较,差异均有统计学意义(p0.05或p0.01)。 (2)间歇低氧4周组+NAC组与单独间歇低氧4周组相比,SOD,GSH-PX升高,MDA降低,差异有统计学意义(p0.05)。但MDA仍高于UC组,SOD、GSH-PX仍低于UC组,差异有统计学意义(P0.05或P0.01)。结论CIH可导致机体氧化应激水平增加,且随着低氧时间延长,机体氧化抗氧化失衡越严重。NAC治疗能够改善CIH导致的氧化应激反应。
[Abstract]:Objective to observe the changes of serum oxidative stress indexes in chronic intermittent hypoxia intermittent mice and to explore the therapeutic effect of N-acetylcystein Nacetylcysteine (N-acetylcystein). Methods Fifty mice were randomly divided into control group (n = 30) and control group (n = 20). They were treated with intermittent hypoxia for 3 days, 1 week, 2 weeks, 3 weeks and 4 weeks, and CIH for 4 weeks, NAC group, 6 rats, respectively. The serum levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and glutathione peroxidase (GSH-PX) were detected by thiobarbituric acid method, hydroxylamine method and chemical colorimetry respectively. The changes of each index and the therapeutic effect of NAC. Results 1) on the 3rd day after intermittent hypoxia, the serum MDA level of mice in the control group was higher than that in the control group, which was significantly higher than that in the UC group (8.98 卤0.50 nmol/mL UC 7.82 卤0.49 nmol / mL). With the prolongation of intermittent hypoxic time, the serum MDA level of mice increased gradually, and there were significant differences between the two groups in each model time period (p0. 05 or p0. 01). In addition, the serum level of GSH-PX in the CIH group was lower than that in the control group after 3 days of intermittent hypoxia, but the difference was not significant (p 0.05), and the serum GSH-PX in the CIH group was 83.30 卤7.32 U / mL 211.5 卤13.39 U / mLrespectively since the first week of hypoxia, which was significantly higher than that in the UC group (95.14 卤8.4 U / mL / L 233.84 卤15.32 U/mL). The level of GSH-PX in serum of CIH group decreased gradually with the prolongation of intermittent hypoxia time, and there were significant differences between the two groups in each model time period (P 0.05 or p 0.01). (2) compared with intermittent hypoxia for 4 weeks, the GSH-PX level in NAC group was significantly lower than that in intermittent hypoxia group for 4 weeks, and the difference was statistically significant (P 0.05). However, MDA was still higher in UC group than in UC group, and GSH-PX was still lower than that in UC group (P 0.05 or P 0.01). Conclusion CIH can increase the level of oxidative stress, and with the prolongation of hypoxic time, the more serious the imbalance of oxidative oxidation. NAC can improve the oxidative stress response induced by CIH.
【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R766.43

【引证文献】