鼻咽癌血清的拉曼光谱研究

发布时间：2018-04-26 11:42

本文选题：激光拉曼光谱 + 鼻咽癌　；参考：《福建医科大学》2010年硕士论文

【摘要】： 目的研究激光拉曼光谱技术对于鼻咽癌血清早期筛查诊断的应用价值。方法从2009年5月到2009年12月,对我院收治的29例经病理证实为鼻咽癌的初诊患者和17例健康志愿者抽取外周静脉血2-4ml于血常规管中。所有的血液样品经离心后取上清液获得血清样品。采用inVia型共聚焦显微拉曼光谱仪进行测量,每个样品测5个点。将同一样品所测量的5条光谱进行平均以代表一个样品的血清拉曼光谱信号。最后将鼻咽癌患者及健康志愿者两组拉曼光谱信号数据、鼻咽癌血清EBV-DNA阴性及阳性两亚组的拉曼光谱数据采用成组t检验,主成分分析,判别分析等进行统计学分析处理。结果鼻咽癌患者和健康志愿者两组血清平均拉曼光谱的谱峰基本相似,但多数谱峰位置的频移差别有统计学意义。常见谱峰I1445、I1651的强度及I1445%qI1651比值的差别无统计学意义(P值分别为:0.08、0.51、0.41)。进一步采用主成分降维处理后予判别分析,两组血清的回顾性判别敏感性为100%,特异性为100%,误判概率为0%,而交叉验证判别敏感性为100%,特异性为93.1%(27/29),误判概率为4.3%(2/46)。鼻咽癌血清EBV-DNA阴性及阳性两亚组血清回顾性判别敏感性为91.7%,特异性为90.9%,误判概率为8.7%(2/23),而交叉验证判别敏感性41.7%(5/12),特异性45.5%(5/11),误判概率为56.5%(13/23)。结论激光拉曼光谱技术可很好的区分鼻咽癌血清及正常人血清,尚无法区分鼻咽癌患者EBV-DNA阴性与EBV-DNA阳性的血清。虽然我们的研究结果令人鼓舞,但仍需要进一步的大宗病例进行验证。
[Abstract]:Objective to study the value of laser Raman spectroscopy in early diagnosis of nasopharyngeal carcinoma (NPC). Methods from May 2009 to December 2009, 29 patients with nasopharyngeal carcinoma (NPC) and 17 healthy volunteers were treated in our hospital. The peripheral venous blood 2-4ml was extracted from the blood routine tube. All blood samples were taken from supernatant after centrifugation to obtain serum samples. InVia confocal microscopy Raman spectrometer was used to measure 5 points for each sample. The 5 spectra measured by the same sample were averaged to represent the serum Raman spectrum signal of one sample. At last, the Raman signal data of NPC patients and healthy volunteers, and the Raman spectrum data of serum EBV-DNA negative and positive subgroups of NPC were analyzed statistically by group t test, principal component analysis and discriminant analysis. Results the average Raman spectra of patients with nasopharyngeal carcinoma and healthy volunteers were similar, but the frequency shift of most peaks was statistically significant. There was no significant difference in the intensity and I1445%qI1651 ratio of the common spectral peak I1445 / I1651 (P = 0.08 / 0.51 / 0.41). The retrospective discriminant sensitivity of the two groups was 100, the specificity was 100, the probability of misjudgment was 0, the sensitivity of cross-validation was 100, the specificity was 93.1% 27 / 29, the probability of misjudgment was 4.33%. The sensitivity of retrospective discriminant was 91.7, the specificity was 90.9, the probability of misjudgment was 8.7 / 23 / 23, the sensitivity of cross-validation was 41.7 / 12, the specificity was 45.5 / 11, and the probability of misjudgment was 56.513 / 230.The results showed that the sensitivity of serum EBV-DNA negative and positive was 91.7%, the specificity was 90.9%, the probability of misjudgment was 8.7% / 23%, and the sensitivity of cross-validation was 41.7% / 12%, and the specificity was 45.5% / 11%. Conclusion Laser Raman spectroscopy can distinguish NPC serum from normal serum, but can not distinguish EBV-DNA negative serum from EBV-DNA positive serum. While our findings are encouraging, further large numbers of cases are needed.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.63

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