SOM模型大鼠中耳黏膜及耳积液中TNF-α的表达及抗TNF-α治疗的机制研究

发布时间：2018-04-29 00:11

本文选题：肿瘤坏死因子 + 分泌性中耳炎　；参考：《中国现代医学杂志》2014年18期

【摘要】：目的探讨抗TNF-α单克隆抗体对大鼠分泌性中耳炎(SOM)的治疗作用及其机制。方法应用随机数字表法将90只健康成年SD大鼠分成三组:对照组、SOM组、抗TNF-α治疗组,每组又根据SOM制成后不同时点,再分为6 h、1 d、3 d、7 d和14 d组,每组6只动物。HE染色后观察中耳黏膜上皮组织病理变化,应用免疫组织化学法检测中耳黏膜上皮组织中TNF-α的表达情况,并采用酶联免疫吸附(ELISA法)检测大鼠耳积液(MEE)中TNF-α的表达水平。结果制模后各时相,对照组标本中无TNF-α的阳性表达,而SOM组和抗TNF-α治疗组中耳黏膜上皮细胞中TNF-α表达灰度值均呈逐渐升高趋势,且较对照组均显著增强(P0.05)。制模后各时相SOM组和抗TNF-α治疗组之间比较,除6 h组外,抗TNF-α治疗组其余3个时相中耳黏膜上皮细胞中TNF-α表达灰度值均明显低于SOM组(P0.05)。制模后各时相SOM组MEE中TNF-α的表达水平均高于对照组,呈逐步上升趋势,差异均有显著性(P0.05)。制模后各时相抗TNF-α治疗组MEE中TNF-α蛋白含量的变化曲线与SOM组相似。两组间不同时间点比较,抗TNF-α治疗组MEE中TNF-α蛋白含量明显均低于SOM组(P0.05)。结论抗TNF-α治疗通过拮抗TNF-α的炎症介导作用而抑制中耳炎症及免疫反应,有助于减轻急性期炎症反应和阻止慢性SOM的发生。
[Abstract]:Objective to investigate the therapeutic effect of anti-TNF- 伪 monoclonal antibody on secretory otitis media in rats and its mechanism. Methods 90 adult Sprague-Dawley rats were randomly divided into three groups: control group and anti-TNF- 伪 treatment group. Each group was divided into 6 h / 1 d ~ 3 d group and 14 d group at different time points after SOM. The pathological changes of middle ear mucosal epithelium were observed after HE staining, and the expression of TNF- 伪 in middle ear mucosa was detected by immunohistochemical method. The expression of TNF- 伪 in rat ear effusion was detected by enzyme-linked immunosorbent assay (Elisa). Results there was no positive expression of TNF- 伪 in the control group at each time after model making, but the gray level of TNF- 伪 expression in the middle ear epithelial cells of SOM group and anti-TNF- 伪 treatment group was increased gradually, and the expression of TNF- 伪 was significantly enhanced than that of the control group (P 0.05). Compared with anti-TNF- 伪 treatment group and SOM group after model making, the expression of TNF- 伪 in the other three phases of anti-TNF- 伪 treatment group was significantly lower than that in SOM group (P 0.05) except for 6 h. The expression level of TNF- 伪 in MEE of SOM group was higher than that of control group, and the expression of TNF- 伪 in MEE was increased gradually, and the difference was significant (P 0.05). The change curve of TNF- 伪 protein content in MEE of anti-TNF- 伪 treatment group was similar to that of SOM group. The content of TNF- 伪 protein in MEE of anti-TNF- 伪 treatment group was significantly lower than that of SOM group at different time points. Conclusion Anti-TNF- 伪 therapy can inhibit otitis media and immune response by antagonizing the inflammatory mediating effect of TNF- 伪, which can alleviate the inflammatory reaction in acute stage and prevent the occurrence of chronic SOM.
【作者单位】：沈阳医学院附属第二医院耳鼻喉科;
【基金】：沈阳市科技计划项目(No:F12-193-9-31)
【分类号】：R764.21

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