l5-羟基前列腺素脱氢酶在鼻咽癌中的表达

发布时间：2018-05-05 22:22

本文选题：鼻咽癌 + 15-PGDH　；参考：《广州医学院》2010年硕士论文

【摘要】：鼻咽癌是我国常见的头颈部恶性肿瘤,其发病机制不十分明确,与其他肿瘤一样,鼻咽癌的发生是一个多因素、多阶段、多步骤的复杂过程,它与病毒、环境因素紧密相关,也与生物遗传、化学、物理因素有密切关系。鼻咽癌位置比较隐蔽,早期诊断率低,治疗目前以放疗为主,但仍反应大,治愈率低,因此鼻咽癌的诊断和治疗仍然面临许多挑战。前列腺素广泛存在于许多组织中,被发现有致癌作用。COX-2是催化花生四烯酸转化为前列腺素(PGs)的关键酶,近年的研究表明COX-2在鼻咽癌中异常高表达,对生长、增殖和转移有促进作用。15-PGDH是前列腺素(PGs)和相关廿烷类生物降解、灭活的关键酶,在多种正常组织中表达,对肿瘤有抑制作用,可降解PGE2,对COX-2也有天然拮抗作用。但是关于15-PGDH在鼻咽部正常组织和鼻咽癌中表达的情况如何目前还无这方面的报道,本研究通过免疫组化和Western blot观察15-PGDH在鼻咽癌和鼻咽正常组织的表达情况,对鼻咽癌细胞是否有抑制作用。目的: 本研究通过免疫组化和Western blot来观察15-PGDH在鼻咽癌和鼻咽正常组织的表达情况,探讨对鼻咽癌细胞是否有抑制作用。方法: 收集广州医学院第二附属医院经病理科确诊的鼻咽组织标本,分成四组:鼻咽正常组、T1T2期鼻咽癌组、T3T4期鼻咽癌组、鼻咽癌淋巴结转移组。通过免疫组化和Western blot观察15-PGDH在各组的表达情况。结果: 免疫组化检测:25例正常鼻咽组织中有15例(60%)15-PGDH呈强阳性染色,8例(32%)15-PGDH呈弱阳性染色,2例(8%)15-PGDH表达缺失;47例鼻咽癌组织中,28例(59.57%)15-PGDH表达缺失,19例(40.43%)15-PGDH呈弱阳性染色,0例15-PGDH呈强阳性染色,P0.05,15-PGDH的表达有显著差异。Western blot印迹分析发现鼻咽癌组11例(37%)可见较弱的特异性条带,19例(63%)无条带;正常组7(70%)例可见特异性强阳性条带,3例(30%)见较弱的特异性条带。与鼻咽正常组相比,鼻咽癌各组15-PGDH表达均很低。结论:1、15-PGDH在鼻咽正常组织中高表达,在鼻咽癌中低表达或阴性表达。 2、鼻咽癌越晚期,15-PGDH的表达越低。 3、15-PGDH对鼻咽癌细胞可能有抑制作用。
[Abstract]:Nasopharyngeal carcinoma (NPC) is a common malignant tumor of head and neck in China. The pathogenesis of nasopharyngeal carcinoma is not very clear. Like other tumors, nasopharyngeal carcinoma is a complex process of multi-factor, multi-stage and multi-step, which is closely related to virus and environmental factors. It is also closely related to biologic, chemical and physical factors. The location of nasopharyngeal carcinoma is relatively concealed, the early diagnosis rate is low, the treatment is mainly radiotherapy at present, but the response is still large, the cure rate is low, so the diagnosis and treatment of nasopharyngeal carcinoma is still facing many challenges. Prostaglandins are widely found in many tissues. COX-2 is found to be a key enzyme in catalyzing the conversion of arachidonic acid to prostaglandin prostaglandins (PGs). Recent studies have shown that COX-2 is highly expressed in nasopharyngeal carcinoma. Proliferation and Metastasis. 15-PGDH is the key enzyme of prostaglandin prostaglandins (PGs) and related eicosane biodegradation and inactivation. It is expressed in many normal tissues and can inhibit tumor, degrade PGE2, and has natural antagonism to COX-2. However, there is no report on the expression of 15-PGDH in normal nasopharynx and nasopharyngeal carcinoma. In this study, the expression of 15-PGDH in nasopharyngeal carcinoma and nasopharyngeal normal tissue was observed by immunohistochemistry and Western blot. Whether has the inhibitory effect to the nasopharyngeal carcinoma cell. Objective: In this study, immunohistochemistry and Western blot were used to observe the expression of 15-PGDH in nasopharyngeal carcinoma (NPC) and nasopharynx (NPC), and to investigate the inhibitory effect of 15-PGDH on nasopharyngeal carcinoma (NPC) cells. Methods: Nasopharyngeal tissue specimens from the second affiliated Hospital of Guangzhou Medical College were collected and divided into four groups: normal nasopharynx group with T 1T 2 stage nasopharyngeal carcinoma, stage T 3T 4 nasopharyngeal carcinoma group and nasopharyngeal carcinoma lymph node metastasis group. The expression of 15-PGDH in each group was observed by immunohistochemistry and Western blot. Results: Immunohistochemical analysis of 15 / 25 normal nasopharynx tissues with strong positive staining for 15-PGDH in 8 cases and weak positive staining for 15-PGDH in 2 cases in 25 normal nasopharynx tissues and in 47 cases of nasopharyngeal carcinoma (NPC), 28 cases (19 cases) with deletion of 15-PGDH expression (40.4315-PGDH) and 19 cases (40.4315-PGDH) with weak positive staining of 15-PGDH (0 cases) and 47 cases of nasopharynx carcinoma (NPC) tissues showed weak positive staining of 15-PGDH. There was a significant difference in the expression of 15-PGDH by positive staining. Western blot blot analysis showed that there were weak specific bands in 11 cases of nasopharyngeal carcinoma (19 cases, 63%). In the normal group, there were 3 cases with strong positive bands and 30 cases with weak specific bands. Compared with normal nasopharynx group, the expression of 15-PGDH in nasopharyngeal carcinoma group was very low. Conclusion the expression of 15-PGDH is high in normal nasopharynx and low or negative in nasopharyngeal carcinoma. 2, the lower the expression of 15-PGDH was in the advanced stage of nasopharyngeal carcinoma. 315-PGDH may have inhibitory effect on nasopharyngeal carcinoma cells.
【学位授予单位】：广州医学院
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R739.63

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