MicroRNA-451通过靶向RAB14抑制鼻咽癌细胞的生长、侵袭和迁移能力

发布时间：2018-05-07 23:31

本文选题：鼻咽肿瘤 + microRNA　；参考：《实用医学杂志》2015年03期

【摘要】：目的:探讨mi R-451对鼻咽癌细胞增殖、侵袭和转移能力的影响。方法:选取正常鼻咽上皮细胞株NP69及4株鼻咽癌细胞株CNE-1、CNE-2、5-8F、6-10B,荧光定量PCR检测mi R-451的表达量。选取鼻咽癌细胞株CNE-1和CNE-2,分别转染mi R-451 mimics和阴性对照后,采用MTT法检测鼻咽癌细胞的增殖能力,划痕实验检测其侵袭能力,transwell迁移实验检测其迁移能力。通过生物信息学方法预测mi R-451的靶基因。结果:与正常鼻咽上皮细胞相比,mi R-451在鼻咽癌细胞株中的表达量明显降低。鼻咽癌细胞过表达mi R-451后,其增殖、侵袭和迁移能力均受到抑制。通过靶基因预测数据库预测RAB14为mi R-451的靶基因,并通过荧光素酶报告基因实验、RT-PCR、Western blot实验验证了这一结果。将RAB14低表达后,也在一定程度上抑制了鼻咽癌的增殖、侵袭和转移能力。结论:mi R-451通过靶向RAB14抑制了鼻咽癌细胞的增殖、侵袭和迁移能力。
[Abstract]:Objective: to investigate the effect of mi R 451 on the proliferation, invasion and metastasis of nasopharyngeal carcinoma cells. Methods: the normal nasopharyngeal epithelial cell line NP69 and four nasopharyngeal carcinoma cell lines CNE-1, CNE-2 and 5-8FN6-10B were used to detect the expression of miR-451 by fluorescence quantitative PCR. Nasopharyngeal carcinoma (NPC) cell lines CNE-1 and CNE-2 were transfected with mi R-451 mimics and negative control, respectively. The proliferation ability of nasopharyngeal carcinoma cells was detected by MTT assay, and the invasion ability was detected by scratch test and transwell migration assay was used to detect migration ability of nasopharyngeal carcinoma cells. The target gene of mi R 451 was predicted by bioinformatics. Results: compared with normal nasopharyngeal epithelial cells, the expression of mil R-451 in nasopharyngeal carcinoma cell lines was significantly decreased. After overexpression of mi R 451, the proliferation, invasion and migration of nasopharyngeal carcinoma cells were inhibited. The target gene RAB14 was predicted to be a target gene of miR-451 by target gene prediction database, and the results were verified by RT-PCRV Western blot experiment of luciferase reporter gene. The low expression of RAB14 also inhibited the proliferation, invasion and metastasis of nasopharyngeal carcinoma to some extent. Conclusion the cell proliferation, invasion and migration of nasopharyngeal carcinoma cells were inhibited by targeting RAB14.
【作者单位】：南方医科大学南方医院肿瘤放射治疗科;南方医科大学南方医院泌尿外科;
【基金】：广东省教育厅科研项目(编号:2013KJCX0038) 2012国家教育部博士学科点项目(编号:20114433110015) 南方医院2013年度高层次课题匹配基金项目(编号:201345)
【分类号】：R739.63

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