ARMS2在人眼不同部位视网膜色素上皮细胞中的表达

发布时间：2018-05-12 11:40

本文选题：年龄相关性黄斑变性易感基因2 + 黄斑部　；参考：《济南大学》2011年硕士论文

【摘要】：目的研究年龄相关性黄斑变性易感基因2 (age—related maculopathy susceptibility 2 gene ARMS2)在人眼黄斑部和周边部视网膜色素上皮细胞(retinal pigment epithelial RPE)中的表达水平,以探讨其参与年龄相关性黄斑变性的可能性。方法 1.对照实验研究。采用人眼角膜移植剩余眼后段材料,由(青岛眼科医院眼库提供),分别于黄斑部及周边部取材进行原代RPE培养,共5只眼。 2.通过western-blot检测ARMS2在源于不同部位的RPE中蛋白表达水平。 3.提取RNA,应用逆转录聚合酶链反应检测ARMS2基因表达变化情况。 4.统计学分析所有数据采用Image J及SSPS13.0统计学软件处理数据,采用△△Ct法计算RQ值,配对t检验及方差分析进行统计学分析,P0.05为差异有统计学意义。结果 Western-blot显示各组周边部RPE中ARMS2蛋白条带与GAPDH(内参照)的灰度比值的相对表达量分别为0.57、0.47、0.42、0.58、0.51,较各组对应黄斑部降低(P0.05)。RT-PCR检测结果显示各组ARMS2RQ值间比较有统计学意义(t=4.671,P0.05)。黄斑部蛋白及m RNA的表达均高于周边部,且差异具有统计学意义。结论黄斑部RPE细胞中ARMS2基因及蛋白表达水平均高于周边部,该基因可能参与了年龄相关性黄斑变性的发生。
[Abstract]:Purpose To study the expression of age-related maculopathy susceptibility 2 gene ARMS2) in retinal pigment epithelial cells (RPE) of human eyes, and to explore the possibility of its involvement in age-related macular degeneration. Method 1. Comparative experimental study. The residual posterior segment of human cornea transplantation was used. The primary RPE culture was performed in the macular region and the peripheral part of the cornea, which was provided by the eye bank of Qingdao Eye Hospital. 2. Western-blot was used to detect the expression of ARMS2 in RPE from different sites. 3. ARMS2 gene expression was detected by reverse transcriptase polymerase chain reaction (RT PCR). 4. All data were analyzed by Image J and SSPS13.0 software. Ct method was used to calculate RQ value. There was significant difference in pairing t test and variance analysis (P0.05). Result The relative expression of ARMS2 protein band and GAPDH (internal reference) in the peripheral RPE of each group by Western-blot was 0.57 ~ 0.472 ~ 0.42 ~ 0.58 ~ 0.51, respectively, which was significantly higher than that of the corresponding macular region in each group. The results of RT-PCR showed that the ARMS2RQ value of each group was 4.671P0.05a significantly higher than that of each group. The expression of macular protein and m RNA was higher than that of peripheral area, and the difference was statistically significant. Conclusion The expression level of ARMS2 gene and protein in macular RPE cells was higher than that in peripheral RPE cells, which may be involved in the occurrence of age-related macular degeneration.
【学位授予单位】：济南大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R774.1

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