多重MSP检测早期鼻咽癌甲基化基因的初步探索

发布时间：2018-05-14 09:13

本文选题：早期鼻咽癌 + 甲基化特异性PCR　；参考：《实用医学杂志》2014年24期

【摘要】：目的:探讨多重MSP(multiplex-MSP)检测基因甲基化在鼻咽癌早期诊断中的作用。方法:通过MSP方法对35例Ⅰ期鼻咽癌标本18个基因的甲基化状态进行验证;同时通过条件摸索建立多重MSP方法,尝试实现多个基因甲基化状态的快速检测。结果:甲基化率超过50%的基因有12个;多重MSP检测中仅1组合的非甲基化特异性产物(U片段)扩增效果没有达到预期结果 ,余3个组合电泳结果与甲基化特异性PCR相符。结论:采取合适的组合进行多重MSP扩增可获得理想结果 ,其结果与MSP以及甲基化芯片扫描结果相似,是一种效率高,成本较低的实验方法。
[Abstract]:Objective: to investigate the role of multiple mSPex-MSPs in the early diagnosis of nasopharyngeal carcinoma (NPC). Methods: the methylation status of 18 genes in 35 patients with stage I nasopharyngeal carcinoma was verified by MSP method, and the multiple MSP method was established to detect the methylation status of multiple genes. Results: there were 12 genes with a methylation rate of more than 50%, and only one non-methylation specific product (U fragment) in multiple MSP assay was not as good as expected, and the results of the remaining 3 combinations were consistent with methylation specific PCR. Conclusion: the ideal results can be obtained by using suitable combination of multiple MSP amplification. The results are similar to those of MSP and methylation chip scanning. It is an experimental method with high efficiency and low cost.
【作者单位】：广东省人民医院(广东省医学科学院)耳鼻咽喉头颈外科;
【基金】：广州市科技计划项目(编号:2011J4300067)
【分类号】：R739.63

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