量子点偶联RGD用于喉癌血管的靶向活体成像

发布时间：2018-05-16 10:58

本文选题：医用光学 + 量子点　；参考：《中国激光》2014年05期

【摘要】：研究了偶联环状精氨酸-甘氨酸-天冬氨酸-D-苯丙氨酸-赖氨酸[c(RGDfK)]肽段的CdSe/ZnS量子点(QDRGD)对喉癌血管靶向成像。利用羧基与氨基反应将c(RGDfK)肽段与QD偶联;采用荧光分光光度计对QD-RGD在RMPI1640培养基和小鼠血清溶液中的光谱稳定性进行了检测;利用荧光显微镜检测QD-RGD对Hep-2细胞和MCF-7细胞上整合素αvβ3的靶向性;最后将QD-RGD尾静脉注射到小鼠体内,检测了其对皮脊翼视窗中喉癌血管的靶向性。结果表明QD-RGD的发射光谱在RMPI1640培养基4h内没有明显变化,在小鼠血清中24h内发射光谱的荧光强度仅下降了20%;对细胞荧光成像表明QD-RGD能特异性与细胞表面的整合素αvβ3结合;血管成像表明QD-RGD在注射2h后聚集在喉癌局部血管,24h后QD-RGD从血管中移除。该研究表明QD-RGD能用于活体喉癌肿瘤血管靶向成像,这为喉癌的靶向诊断和靶向治疗研究提供了参考。
[Abstract]:CdSe/ZnS quantum dots (QDRGDs) coupled with cyclic arginine glycine aspartic acid D- phenylalanine-lysine peptide were studied for vascular targeting imaging of laryngeal carcinoma. The carboxyl group and amino group reaction were used to couple cGDfK peptide with QD, and the spectral stability of QD-RGD in RMPI1640 medium and mouse serum solution was determined by fluorescence spectrophotometer. The targeting ability of QD-RGD to integrin 伪 v 尾 3 in Hep-2 cells and MCF-7 cells was detected by fluorescence microscope, and finally, the QD-RGD tail vein was injected into mice to detect its targeting to laryngeal cancer vessels in flanking window. The results showed that the emission spectrum of QD-RGD did not change significantly within 4 h of RMPI1640 medium, and the fluorescence intensity of emission spectrum decreased only 20% in mouse serum within 24 h, and the fluorescence imaging showed that QD-RGD could specifically bind to integrin 伪 v 尾 3 on cell surface. Angiography showed that QD-RGD was removed from the laryngeal carcinoma after 2 h of QD-RGD aggregation in the local vessels of laryngeal carcinoma. This study shows that QD-RGD can be used in angiographic imaging of laryngeal carcinoma in vivo, which provides a reference for the study of targeted diagnosis and therapy of laryngeal carcinoma.
【作者单位】：深圳大学光电工程学院教育部/广东省光电子器件与系统重点实验室;深圳大学医学院深圳市生物医学工程重点实验室;
【基金】：国家973计划(2012CB85802) 国家自然科学基金(61235012,61335001) 广东省科技创新项目(2012KJCX0094)
【分类号】：R739.65

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