NS-398对离体培养的大鼠视网膜小胶质细胞TNF-a和COX-2表达的影响

发布时间：2018-05-22 08:27

本文选题：视网膜小胶质细胞 + 脂多糖　；参考：《中南大学》2010年硕士论文

【摘要】： 目的：探讨环氧合酶-2选择性抑制剂NS-398对脂多糖刺激下的离体培养的大鼠视网膜小胶质细胞TNF-a和COX-2表达的调节作用。方法：脂多糖刺激离体培养的第四代SD新生大鼠视网膜小胶质细胞,将培养细胞分为五组：A组空白对照组：实验组根据含不同浓度的NS-398分为：B组实验对照组(10μg/LLPS);C组低浓度作用组(50μmol/L NS-398+10μg/LLPS);D组中浓度作用组(100μmol/LNS-398+ 10μg/LLPS);E组高浓度作用组(200μmol/L NS-398+10μg/LLPS);分别作用24及48小时后,RT-PCR半定量检测各组中TNF-a和COX-2的表达,并进行统计学分析。结果： 1.视网膜小胶质细胞分别作用24h及48h后,各组间TNF-a的mRNA表达量的差异有统计学意义(P0.05),随培养液中NS-398浓度的增加,LPS刺激后视网膜小胶质细胞中TNF-a的mRNA的表达量逐渐降低。同组视网膜小胶质细胞在不同时间的TNF-a的mRNA表达量除A组(空白对照组)差异无统计学意义外(P0.05),余差异有统计学意义(P0.05)。 2.视网膜小胶质细胞分别作用24h及48h后,各组间COX-2的mRNA表达量的差异有统计学意义(P0.05),随培养液中NS-398浓度的增加,LPS刺激后视网膜小胶质细胞中COX-2的mRNA的表达量逐渐降低。同组视网膜小胶质细胞在不同时间的COX-2的mRNA表达量除A组(空白对照组)差异无统计学意义外(P0.05),余差异有统计学意义(P0.05)。结论： 1.视网膜小胶质细胞可以被激活释放炎症相关的细胞因子TNF-a和COX-2,引起一系列的炎症反应。 2.环氧合酶-2选择性抑制剂NS-398抑制视网膜小胶质细胞表达TNF-a和COX-2,继而抑制炎症反应。 3.NS-398对视网膜小胶质细胞的抑制作用呈时间剂量依赖型。
[Abstract]:Objective: To investigate the effects of cyclooxygenase-2 selective inhibitor (NS-398) on the expression of TNF-a and COX-2 in cultured rat retinal microglia stimulated by lipopolysaccharide (LPS). Methods: Lipopolysaccharide stimulated retinal microglia of the fourth generation SD neonatal rats in vitro. The cultured cells were divided into five groups: control group: the experimental group was divided into two groups according to different concentration of NS-398: the control group was divided into two groups: the control group (n = 10 渭 g / L / L) treated with 10 渭 g / L LPSN / C, the control group (n = 50 渭 mol/L NS-398, n = 10 渭 g / L / L / L) and the middle concentration group (n = 100 渭 mol/LNS-398 = 10 渭 g / L / L / L / L / L / L) respectively, and the control group (n = 100 渭 mol/LNS-398 = 10 渭 g / L / L / L) were divided into two groups (n = 10). After 24 and 48 hours of exposure, semi-quantitative RT-PCR was used to detect the expression of TNF-a and COX-2 in each group. Statistical analysis was carried out. Results: 1. After treated with TNF-a for 24 and 48 hours, the expression of mRNA in retinal microglia was significantly different (P 0.05), and the expression of TNF-a mRNA in retinal microglia decreased gradually with the increase of NS-398 concentration in culture medium. The mRNA expression of retinal microglia at different time in the same group was significantly higher than that in group A (P 0.05) except that in group A (blank control group). 2. After treated with COX-2 for 24 and 48 hours, the expression of mRNA in retinal microglia was significantly different (P 0.05), and the expression of COX-2 mRNA in retinal microglia decreased gradually with the increase of NS-398 concentration in culture medium. The mRNA expression of retinal microglia at different time in the same group was significantly higher than that in group A (P 0.05) except that in group A (blank control group). Conclusion: 1. Retinal microglia can be activated to release inflammatory cytokines TNF-a and COX-2, causing a series of inflammatory responses. 2. Cyclooxygenase-2 selective inhibitor NS-398 inhibits the expression of TNF-a and COX-2 in retinal microglia and then inhibits inflammation. The inhibitory effect of 3.NS-398 on retinal microglia was time-and-dose-dependent.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R587.2;R774.1

【参考文献】