川芎对庆大霉素致聋豚鼠耳蜗氧自由基生成的影响

发布时间：2018-05-24 09:39

本文选题：川芎 + 庆大霉素　；参考：《听力学及言语疾病杂志》2014年01期

【摘要】：目的研究川芎注射液(tetramethylpyrazine,TMP)对庆大霉素致聋豚鼠耳蜗内氧自由基生成的影响,探讨TMP对急性药物性聋的干预作用及机制。方法选择正常的健康豚鼠60只随机分成正常对照组(10只)、TMP组(10只)、庆大霉素组(GM组,20只)、GM+TMP组(20只)。GM组每日腹腔注射硫酸庆大霉素120mg/kg,TMP组每日腹腔注射盐酸川芎嗪40mg/kg,GM+TMP组同时注射硫酸庆大霉素和盐酸川芎嗪,剂量同GM组和TMP组;正常对照组则每日腹腔注射等量生理盐水,均连续用药10天,每日一次。各组动物于用药前及停药后第1天分别行ABR测试,停药后第1天测试ABR后用羟胺法和硫代巴比妥法检测各组豚鼠耳蜗组织中超氧化物歧化酶(SOD1、SOD2、T-SOD)活力和丙二醛(MDA)含量。结果用药10天后GM组ABR反应阈明显升高,与用药前、正常对照组及GM+TMP组比较差异有统计学意义(P0.01),TMP组用药前后ABR反应阈差异无统计学意义(P0.05)。GM组耳蜗组织中MDA含量显著升高,与正常对照组、GM+TMP组、TMP组比较差异有统计学意义(P0.05);GM+TMP组、TMP组与正常对照组之间耳蜗MDA含量比较差异无统计学意义(P0.05)。GM+TMP组耳蜗组织中T-SOD活性明显高于GM组,差异有统计学意义(P0.05),GM组其活性低于正常组(P0.05)。GM组耳蜗组织中SOD1活性明显下降,且与正常对照组比较差异有统计学意义(P0.05),GM+TMP组耳蜗组织中SOD1、SOD2活性明显高于GM组(P0.05)。结论 TMP可通过提高耳蜗组织内SOD的活力,以防止脂质的过氧化,从而减轻庆大霉素对耳蜗的早期损伤。
[Abstract]:Objective to study the effect of tetramethylpyrazine (TMP) on the formation of oxygen free radicals in the cochlea of gentamicin induced deafness guinea pigs and explore the effect and mechanism of TMP on acute drug-induced deafness. Methods 60 healthy guinea pigs were randomly divided into normal control group (10 rats), group TMP (10), gentamicin group (group GM, 20 rats), group GM+TMP (20). Only group.GM was intraperitoneally injected with gentamicin sulfate 120mg/kg daily, group TMP was intraperitoneally injected with tetramethylpyrazine hydrochloride 40mg/kg, group GM+TMP was injected with gentamicin sulfate and ligustrazine hydrochloride in group GM and TMP group, and normal control group was injected with equal amount of saline daily for 10 days, once a day. ABR tests were performed on the first day after withdrawal, and after the first day after stopping the drug, the activity of superoxide dismutase (SOD1, SOD2, T-SOD) and the content of malondialdehyde (MDA) were detected by hydroxylamine and thiobarbitone in the cochlear tissues of each group. The results showed that the ABR reaction threshold of the GM group increased significantly after 10 days of drug use, and was worse than the normal control group and the GM+TMP group before the medication. There was no statistical significance (P0.01). There was no significant difference in the threshold of ABR reaction in group TMP (P0.05). The content of MDA in the cochlear tissue of.GM group was significantly higher than that of the normal control group, GM+TMP group and TMP group (P0.05), and there was no statistical difference between the GM+TMP group and the TMP group and the normal control group. The activity of T-SOD in the cochlear tissue of group GM+TMP was significantly higher than that of the GM group (P0.05). The activity of GM in the GM group was lower than that in the normal group (P0.05), and the activity of SOD1 in the cochlear tissue was significantly lower than that of the normal control group (P0.05), and the SOD1 in the cochlear tissue of the GM+TMP group was significantly higher than that in the GM group. It is possible to prevent the early damage of cochlea by gentamicin by increasing the activity of SOD in the cochlea tissue to prevent lipid peroxidation.
【作者单位】：浙江中医药大学耳聋康复研究所;
【基金】：国家自然科学基金面上项目(81173309)
【分类号】：R764

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