2型糖尿病视网膜病变患者外周血单个核细胞中PEDF与TNF-α水平的变化

发布时间：2018-05-27 07:13

本文选题：色素上皮衍生因子 + 外周血单个核细胞　；参考：《中国病理生理杂志》2014年02期

【摘要】：目的:色素上皮衍生因子(PEDF)是影响糖尿病视网膜病变(DR)发生发展的重要因子,本研究观察2型糖尿病视网膜病变患者外周血单个核细胞(PBMCs)中PEDF、磷酸化NF-κB p65及TNF-α的表达,探讨DR的发病机制。方法:选取2011年10月~2013年4月在我院内分泌科住院确诊为2型糖尿病的患者(DM组)108例,并根据眼底造影结果将患者分为无糖尿病视网膜病变组(NDR组)52例和糖尿病视网膜病变组(DR组)56例,同期糖耐量实验正常者52例作为对照组(NC组)。密度梯度离心法收集患者PBMCs,提取总蛋白,Western blotting检测3组患者PBMCs中PEDF、磷酸化NF-κB p65和TNF-α蛋白表达水平,ELISA测定血浆PEDF与TNF-α水平,收集患者体检指标并测量患者生化指标。结果:Western blotting检测结果显示NDR组和DR组PEDF、磷酸化NF-κB p65和TNF-α蛋白水平明显高于NC组(P0.05);DR组TNF-α蛋白水平明显高于NDR组(P0.05),DR组PEDF及磷酸化NF-κB p65蛋白水平略高于NDR组,差异无统计学意义(P0.05);NDR组和DR组血浆PEDF与TNF-α水平均明显高于NC组(P0.05),DR组明显高于NDR组(P0.05)。在糖尿病组,血浆PEDF水平与TNF-α及血尿酸水平呈正相关(r=0.39,r=0.25,P0.05)。结论:本研究首次证实PBMCs中可表达PEDF;2型糖尿病患者PBMCs中PEDF表达水平增加,TNF-α及血浆PEDF的表达水平上调,PEDF可能通过与炎症因子TNF-α相互作用影响DR发生发展。
[Abstract]:Objective: pigment epithelium-derived factor (PEDF) is an important factor affecting the development of diabetic retinopathy. The expression of PEDF-, phosphorylated NF- 魏 B p65 and TNF- 伪 in peripheral blood mononuclear cells (PBMCs) of patients with type 2 diabetic retinopathy was studied. To explore the pathogenesis of Dr. Methods: 108 patients with type 2 diabetes mellitus were selected from October 2011 to April 2013. According to the results of fundus angiography, the patients were divided into two groups: NDR group (n = 52), diabetic retinopathy group (n = 56) and diabetic retinopathy group (n = 56). 52 cases of normal glucose tolerance test were used as control group. Peripheral blood mononuclear cells (PBMCs) were collected by density gradient centrifugation. The levels of PEDFs, phosphorylated NF- 魏 B p65 and TNF- 伪 proteins in PBMCs were detected by Western blotting. The plasma levels of PEDF and TNF- 伪 were measured by Elisa. The indexes of physical examination were collected and the biochemical indexes of patients were measured. Results the protein levels of phosphorylated NF- 魏 B p65 and TNF- 伪 in NDR group and Dr group were significantly higher than those in P0.05 Dr group and PEDF group and phosphorylated NF- 魏 B p65 protein level in NDR group. There was no significant difference in plasma PEDF and TNF- 伪 levels between the two groups. The levels of plasma PEDF and TNF- 伪 were significantly higher in NC-group than in NDR group. In diabetic group, plasma PEDF level was positively correlated with TNF- 伪 and serum uric acid level. Conclusion: this study demonstrated for the first time that the increased expression of PEDF in PBMCs and the up-regulation of plasma PEDF expression in PBMCs patients with type 2 diabetes mellitus may influence the development of Dr through the interaction with inflammatory factor TNF- 伪.
【作者单位】：中山大学附属第三医院内分泌与代谢病科;
【基金】：广东省科技计划(No.2011B031800155) 广东省自然科学基金资助项目(No.S2011010004811)
【分类号】：R774.1

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