结膜下注射贝伐单抗对兔角膜新生血管的抑制作用

发布时间：2018-05-30 21:06

本文选题：贝伐单抗 + 角膜新生血管　；参考：《暨南大学》2010年硕士论文

【摘要】： 目的：通过对碱烧伤后不同时间结膜下注射贝伐单抗(Bevacizumab),观察角膜新生血管(corneal neovascularization, CNV)形成的转归,探讨结膜下注射贝伐单抗对CNV的影响及最佳给药时间,为该药的临床应用提供实验依据。方法：取新西兰白兔54只,将浸有1mol／L氢氧化钠溶液的圆形滤纸片置于兔右眼角膜中央,制成单眼碱烧伤模型,随机分为A、B、C组,每组18眼,A组碱烧伤后结膜下立即注射bevacizumab 2.5mg (0.1 ml), B组碱烧伤后3d结膜下注射bevacizumab 2.5mg (0.1 ml),C组为空白对照组。观察28d。裂隙灯显微镜下观察角膜新生血管生长情况,行眼前段照相并计算其面积,伤后7、14、28d各组随机取6例角膜行病理组织学及共焦显微镜检查,观察角膜组织炎性细胞浸润情况及角膜新生血管形态学变化。结果：A、B及C组角膜新生血管开始出现的时间分别为(5.9±0.8)d、(3.5±0.6)d及(3.4±1.1)d,其中A明显较C组延长(P0.05),B组与C组比较无显著差异(P=0.068)。伤后各时间点A、B组角膜新生血管的生长面积均明显较C组减少(P0.05),A组与B组角膜新生血管面积比较,差异有显著意义(P0.05)。角膜组织病理学检查显示,C组烧伤区可见大量炎性细胞浸润及新生血管形成,而A组角膜炎性反应较轻,烧伤区无新生血管形成,B组见少量新生血管侵入烧伤区。三组各时间点角膜组织炎性细胞计数有统计学差异(P0.05)。角膜新生血管面积与炎性细胞数有明显相关性(P0.05)。共焦显微镜检查可见角膜组织炎性细胞浸润、新生血管管腔及基质层瘢痕组织形成,治疗组基质层纤维增生程度与瘢痕组织均较对照组轻。结论：结膜下注射贝伐单抗可抑制角膜炎性细胞形成,改善损伤角膜基质,促进角膜愈合,从而减少碱烧伤引起的角膜新生血管的生长,在早期注射能取得更好的疗效。
[Abstract]:Objective: to investigate the effect of subconjunctival injection of bevacizumaban on the formation of corneal neovascularization (CNV) after alkali burn, and to explore the effect of subconjunctival injection of bevacizumab on CNV. To provide experimental basis for the clinical application of the drug. Methods: Fifty-four New Zealand white rabbits were divided into two groups randomly. The circular filter paper soaked with 1mol/L sodium hydroxide solution was placed in the center of the cornea of the right eye to make a monocular alkali burn model. In each group, 18 eyes of group A were injected with bevacizumab 2.5mg 0.1 ml immediately after alkali burn, and group B 3 days after alkali burn with bevacizumab 2.5mg 0.1 ml of bevacizumab 2.5mg as control group. Observe 28 days. The corneal neovascularization was observed under slit lamp microscope. The anterior segment of cornea was photographed and its area was calculated. Six cases of cornea were randomly selected for histopathology and confocal microscopy on day 71428 after injury. The infiltration of inflammatory cells and the morphological changes of corneal neovascularization were observed. Results the onset time of corneal neovascularization in group B and C were 5.9 卤0.8 d, 3.5 卤0.6 d and 3.4 卤1.1 d, respectively. There was no significant difference between group A and group C in prolonging the onset of corneal neovascularization in group A and group C. there was no significant difference between group A and group C in the onset of corneal neovascularization. The area of corneal neovascularization in group A was significantly lower than that in group C at each time point after injury, and there was a significant difference between group A and group B in the area of corneal neovascularization. Corneal histopathological examination showed that a large number of inflammatory cells infiltration and neovascularization were observed in burn area in group C, but the keratitis response was mild in group A, and a small amount of neovascularization invaded burn area in group B without neovascularization in burn area. There was significant difference in the number of inflammatory cells in corneal tissue between the three groups at different time points (P 0.05). There was a significant correlation between corneal neovascularization area and the number of inflammatory cells. Confocal microscopy showed inflammatory cell infiltration in corneal tissue and formation of scar tissue in neovascularization and stromal layer. The degree of fibrous proliferation in stroma and scar tissue in the treatment group were lighter than those in the control group. Conclusion: subconjunctival injection of bevacizumab can inhibit the formation of corneal inflammatory cells, improve corneal stroma injury, promote corneal healing, and reduce corneal neovascularization caused by alkali burn.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R779.1

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