Gpnmb基因在BXD重组近交系小鼠眼组织的表达差异和基因调控网络分析

发布时间：2018-05-31 17:39

本文选题：糖蛋白神经调节肽B相关基因 + 色素性青光眼　；参考：《江苏医药》2014年02期

【摘要】：目的研究色素播散综合征(PDS)和色素性青光眼(PG)致病基因糖蛋白神经调节肽B相关基因(Gpnmb)在BXD重组近交系(BXD RI)小鼠眼组织的表达差异及其基因调控网络。方法采用Affymetrix基因芯片检测确定68个品系的BXD RI小鼠眼组织中Gpnmb基因的表达差异。采用基因表达数量性状基因座(eQTL)分析方法,结合GeneNetwork网站上的在线分析工具,研究差异表达的特征,进而构建Gpnmb基因调控网络。结果 Gpnmb在BXD RI眼组织中平均表达水平为10.92,不同品系小鼠表达量存在差异。Gpnmb基因为顺式作用的eQTL,End3基因是其下游候选基因。基于和Gpnmb高度相关基因的基因网络得以构建。结论构建Gpnmb基因调控网络,为PDS和PG发病机制的深入研究提供线索。
[Abstract]:Objective to study the differential expression of glycoprotein neuromodulatory peptide B related gene (Gpnmb) related genes (Gpnmb) in the BXD recombinant inbred line (BXD RI) mouse eye tissues and its gene regulation network. Methods the Gpnmb gene in the BXD RI mouse eye tissues of 68 strains was determined by Affymetrix gene chip. The analysis method of gene expression quantitative trait loci (eQTL) and the online analysis tool on the GeneNetwork website were used to study the characteristics of the differential expression, and then the Gpnmb gene regulation network was constructed. The average expression level of Gpnmb in the BXD RI eye tissues was 10.92, and the expression of different strain mice was different in the.Gpnmb gene. For the CIS action of eQTL, the End3 gene is the downstream candidate gene. The gene network based on the highly related gene of Gpnmb is constructed. Conclusion the construction of the Gpnmb gene regulation network provides a clue for the in-depth study of the pathogenesis of PDS and PG.
【作者单位】：南通大学附属医院眼科;美国田纳西大学医学中心;
【基金】：江苏省自然科学基金(BK2008186) 南通市社会事业创新与示范计划(HS2011005)
【分类号】：R775

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