纤维生长因子受体1抑制剂PD173074对鼻咽癌细胞系增殖和凋亡的影响

发布时间：2018-06-02 12:12

本文选题：纤维生长因子受体 + PD　；参考：《临床耳鼻咽喉头颈外科杂志》2014年20期

【摘要】：目的:研究纤维生长因子受体1(FGFR1)抑制剂PD173074对于鼻咽癌细胞增殖和凋亡的影响。方法:通过免疫印迹和实时定量PCR研究了FGFR1在多种鼻咽癌细胞系中的表达情况;通过MTT法检测了PD173074对CNE细胞增殖抑制的时间-效应关系和浓度-效应关系;通过免疫印迹研究了PD173074对FGFR1及下游AKT的磷酸化水平的影响;通过Caspase3/9活性检测和免疫印迹研究了PD173074对鼻咽癌细胞凋亡的影响及凋亡途径。结果:在CNE、PONE1和C666-1中,CNE细胞系FGFR1表达最高。10nmol/L PD173074刺激36h以上时能够通过抑制FGFR1和其下游的AKT磷酸化水平而抑制CNE细胞的增殖水平。在CNE细胞系中,PD173074刺激36h以上能够活化Caspase9并进一步激活Caspase3,引起细胞凋亡。结论:PD173074通过抑制FGFR1和下游的AKT磷酸化抑制鼻咽癌细胞系CNE的增殖,并激活内源性凋亡途径激活Caspase9,进而激活Caspase3引起CNE细胞凋亡。
[Abstract]:Aim: to study the effects of PD173074, a FGFR1 inhibitor, on proliferation and apoptosis of nasopharyngeal carcinoma cells. Methods: the expression of FGFR1 in various nasopharyngeal carcinoma cell lines was studied by Western blot and real-time quantitative PCR, and the time-effect and concentration-effect relationships of PD173074 on the proliferation of CNE cells were detected by MTT assay. The effect of PD173074 on the phosphorylation of FGFR1 and downstream AKT was studied by Western blotting, and the effect of PD173074 on apoptosis of nasopharyngeal carcinoma cells was studied by Caspase3/9 activity detection and Western blotting. Results: the expression of FGFR1 was the highest in CNE PONE1 and C666-1 cell lines, and the proliferation of CNE cells could be inhibited by inhibiting the phosphorylation level of FGFR1 and its downstream AKT after stimulation of PD173074 for more than 36 h. In CNE cell line, PD173074 stimulated Caspase9 for more than 36 h and further activated Caspase3, which resulted in apoptosis. Conclusion the cell proliferation of nasopharyngeal carcinoma cell line CNE was inhibited by inhibiting FGFR1 and downstream AKT phosphorylation, and Caspase9 was activated by activation of endogenous apoptosis pathway, and then CNE cell apoptosis induced by Caspase3 was activated by% PD173074.
【作者单位】：日照市东港区人民医院五官科;山东大学齐鲁医院普外科;日照市东港区人民医院小儿科;
【分类号】：R739.63

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