雷帕霉素对缺氧条件下Hep-2细胞增殖及VEGF、HIF-1α表达的影响

发布时间：2018-06-02 13:49

本文选题：喉癌 + 缺氧　；参考：《郑州大学学报(医学版)》2014年06期

【摘要】：目的:探讨雷帕霉素对缺氧条件下人喉癌Hep-2细胞增殖及VEGF、HIF-1α表达的影响。方法:在缺氧条件下,采用MTT法检测0、5、10、20 nmol/L雷帕霉素处理12、24、36、48、60、72 h后Hep-2细胞的增殖活性,ELISA检测0、5、10、20 nmol/L雷帕霉素处理24 h细胞培养上清液VEGF蛋白含量的变化,Western blot检测0、5、10、20nmol/L雷帕霉素处理16 h细胞HIF-1α蛋白表达的变化。结果:缺氧条件下雷帕霉素对Hep-2细胞仍具有明显的增殖抑制作用,且呈浓度和时间依赖性(F浓度=253.132,F时间=213.945,P0.05)。缺氧条件下雷帕霉素可抑制Hep-2细胞VEGF的分泌及HIF-1α蛋白的表达(F=7.045、16.218,P0.05)。结论:雷帕霉素可抑制缺氧条件下Hep-2细胞的增殖,其机制可能与抑制Hep-2细胞VEGF的分泌及HIF-1α蛋白的表达有关。
[Abstract]:Aim: to investigate the effects of rapamycin on proliferation and expression of VEGF HIF-1 伪 in human laryngeal carcinoma Hep-2 cells under hypoxia. Methods: under the condition of hypoxia, The proliferative activity of Hep-2 cells treated with rapamycin for 10 ~ 20 nmol/L was detected by MTT method. The expression of HIF-1 伪 protein in the supernatant of Hep-2 cells treated with rapamycin for 24 h was detected by Elisa. The protein expression of HIF-1 伪 was detected by Western blot in the culture supernatant of the cells treated with rapamycin for 24 h and the protein expression of HIF-1 伪 was detected in the cells treated with 0 ~ 51020 nmol / L rapamycin for 16 h. Results: rapamycin still inhibited the proliferation of Hep-2 cells in a dose-and time-dependent manner. Under hypoxia condition, rapamycin could inhibit the secretion of VEGF and the expression of HIF-1 伪 protein in Hep-2 cells. Conclusion: rapamycin can inhibit the proliferation of Hep-2 cells under hypoxia, and its mechanism may be related to the inhibition of VEGF secretion and HIF-1 伪 protein expression in Hep-2 cells.
【作者单位】：郑州大学第三附属医院耳鼻咽喉科;郑州大学第一附属医院肿瘤科;郑州大学第一附属医院耳鼻咽喉科;
【分类号】：R739.65

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