FSCN1和HGF在鼻内翻性乳头状瘤组织中的表达及其临床意义

发布时间：2018-06-03 03:46

本文选题：鼻内翻性乳头状瘤 + FSCNI　；参考：《郑州大学》2011年硕士论文

【摘要】：背景与目的鼻内翻性乳头状瘤(nasal inverted papilloma, NIP)是耳鼻咽喉头颈外科比较多见的鼻腔鼻窦良性肿瘤,约占鼻腔鼻窦肿瘤的0.4%-4.7%,其男女性别发病率差别较大,男性显著高于女性,城市高于农村。据有关报道显示,随着人类受饮食,生活及周边环境的影响,具有侵袭性、高复发性和易癌变等临床特点。其病因复杂且诱发因素较多,迄今确切的病因尚不清楚,可能为多种因素综合作用所致。随着对信号通路传导系统在肿瘤分子生物学领域的研究,通过干预信号通路传导途径治疗肿瘤已成为生物治疗的新兴领域。 Fascin蛋白属于三叶虫结构蛋白家族中的一员,人类Fascin-1 (FSCN1)基因定位于染色体7p22,在正常组织器官中,FSCN1表达于间叶组织和神经系统,编码一种分子质量为55 kDa的细胞骨架蛋白,可与F-肌动蛋白结合,定位于细胞质张力纤维和细胞膜皱褶边缘的丝状伪足、微棘的核心肌动蛋白束中,Fasein蛋白N端第39位的丝氨酸为蛋白激酶C(PKC)的磷酸化位点,该位点的磷酸化可调节Fascin蛋白与F-actin的结合活性以及细胞膜表面丝状伪足和微棘的形成,提示Fascin蛋白可能在细胞迁移、细胞黏附以及细胞间信息交流等过程中发挥作用,在肿瘤的形成和发展中扮演着重要的角色,促进肿瘤的侵袭和转移。肝细胞生长因子(Hepatocyte growth factor, HGF)是一种多功能因子,生物活性由C-met蛋白介导,能促进肿瘤细胞侵袭及新生血管生成。HGF主要由间质细胞衍生的多功能细胞因子,通过自分泌或旁分泌的形式作用于数种上皮源性的细胞(如肝细胞、肾小管上皮细胞),诱导细胞有丝分裂、刺激细胞移动并且对抗细胞因子诱导的凋亡。HGF-c-Met信号传导通路广泛存在于各种细胞中,对多种组织器官的生长发育有重要的生理调节功能,HGF及其受体的研究,可能会为抗肿瘤转移防治提供一个极有希望的靶点。本研究拟收集在我院耳鼻咽喉科接受手术治疗的病人新鲜组织标本,慢性肥厚性鼻炎的下鼻甲粘膜12例(对照组),鼻内翻性乳头状瘤40例,鼻腔鼻窦鳞癌14例。本实验采用免疫组织化学方法检测FSCN1和HGF在下鼻甲、鼻内翻性乳头状瘤及鼻腔鳞癌组织中的表达,并对结果进行分析,探讨FSCN1和HGF在N IP组织中的表达及其临床意义。材料与方法 1.收集2009年7月-2010年10月在我院耳鼻咽喉科接受手术治疗的病人新鲜组织标本,慢性肥厚性鼻炎的下鼻甲粘膜12例(对照组),鼻内翻性乳头状瘤40例,鼻腔鼻窦鳞癌14例。所有病例术前均未行放疗,化疗等其他相关治疗,临床病历资料完整,术后经病理检查诊断同术前。 2.采用免疫组化SP法分析FSCN和HGF在3种鼻病组织中的阳性表达。 3.采用SPSS13.0统计软件包进行数据分析,X2检验及spearman等级相关分析。以P0.05有统计学意义。结果 1.FSCN1蛋白主要表达于细胞浆,在下鼻甲组织中的阳性表达率为8.3%(1/12),在鼻内翻性乳头状瘤组织中的阳性表达率为52.5%(21/40),在鼻腔鼻窦鳞癌组织中的阳性表达率为78.6%(11/14),三种鼻组织表达差异有统计学意义(P0.05)。 2.HGF主要表达于细胞浆,少数见于细胞核内,在下鼻甲组织中的阳性表达率为8.3%(1/12),在鼻内翻性乳头状瘤组织中的阳性表达率为40.0%(16/40),在鼻腔鼻窦鳞癌组织中的阳性表达率为82.9%(12/14),三种鼻组织表达差异有统计学意义(P0.051)。 3.FSCN1/HGF在鼻内翻性乳头状瘤组织中和鼻腔鼻窦鳞癌组织中的表达呈正相关(P0.05)。结论 1. FSCN1和HGF在鼻内翻性乳头状瘤组织中均高表达,提示FSCN1和HGF在鼻内翻性乳头状瘤的发生和发展中起着重要的作用。 2. FSCN1和HGF均参与鼻内翻性乳头状瘤的形成过程,但与鼻内翻性乳头状瘤患者的病程、年龄及性别等因素无关。 3. FSCN1和HGF在鼻内翻性乳头状瘤中的表达具有相关性,提示二者可能协同参与鼻内翻性乳头状瘤的形成过程。 4. FSCN1和HGF在下鼻甲组织、鼻内翻性乳头状瘤组织及鼻腔鼻窦鳞癌组织中的表达依次升高,提示二者在三种鼻病的转化中起一定促进作用。 5. FSCN1和HGF的表达可反映鼻腔鼻窦肿瘤生物学行为的客观指标,二者过度表达可能是鼻内翻性乳头状瘤恶变为鳞癌的早期事件,通过检测二者在三种鼻病的表达,为预防鼻腔鼻窦良恶性肿瘤的发生及诊疗提供新思路。
[Abstract]:Background and purpose
Nasal inverted papilloma (nasal inverted papilloma, NIP) is a more common benign tumor of the nasal sinus and nasal sinus, which accounts for the 0.4%-4.7% of the nasal sinus tumor. The male and female sex incidence is different, the male is significantly higher than the female, and the city is higher than the countryside. According to the reports, with the human diet, life and the surrounding area The effect of the environment, which has the clinical characteristics of invasive, high relapse and carcinogenesis, is complicated and causes many factors. The exact cause of the cause is not yet clear, and it may be caused by a variety of factors. With the study of the signal pathway conduction system in the field of molecular biology of tumor, the treatment of signal pathway conduction pathway is used. Cancer has become an emerging field of biotherapy.
Fascin protein belongs to a member of the structural protein family of the three leaf worm. The human Fascin-1 (FSCN1) gene is located on the chromosome 7p22. In normal tissues, FSCN1 is expressed in the interleaf tissue and the nervous system, and encodes a cytoskeleton protein of 55 kDa molecular mass. It can be combined with the actin with F- actin and located in the cytoplasm tension fibers and cells. The filamentous pseudo foot on the edge of the membrane folds, in the core actin bundle of the microspines, the thirty-ninth bit serine of the Fasein protein N terminal is the phosphorylation site of the protein kinase C (PKC). The phosphorylation of the site can regulate the binding activity of the Fascin protein with F-actin, and the formation of the filamentous and microspines on the surface of the cell membrane, suggesting that the Fascin protein may be migrated to the cell. Cell adhesion and intercellular information exchange play an important role in the formation and development of the tumor, promoting tumor invasion and metastasis.
Hepatocyte growth factor (HGF) is a multifunctional factor. Bioactivity is mediated by C-met protein, which promotes tumor cells invasion and neovascularization of the multifunctional cytokines derived mainly by interstitial cells and acts on several epithelial derived cells by autocrine or paracrine forms (such as liver cells). Renal tubular epithelial cells), inducing cell mitosis, stimulating cell migration and antagonism cell factor induced apoptosis.HGF-c-Met signal transduction pathway widely exists in various cells, and has important physiological regulating function for the growth and development of various tissues and organs. The study of HGF and its receptor may provide one for the prevention and control of anti tumor metastasis. A very promising target.
This study was to collect fresh tissue specimens of patients undergoing surgical treatment in our department of Otolaryngology, 12 cases of inferior turbinate mucosa of chronic hypertrophic rhinitis (control group), 40 cases of inverted papilloma of the nose, and 14 cases of nasal sinus squamous cell carcinoma. This experiment was conducted by immunohistochemistry to detect FSCN1 and HGF in the inferior turbinate, nasal inverted papilloma and nose. The expression of FSCN1 and HGF in N IP tissues and their clinical significance were analyzed.
Materials and methods
1. a collection of fresh tissue specimens, 12 cases of inferior turbinate mucosa of chronic hypertrophic rhinitis (control group), 40 cases of nasal inverted papilloma and 14 cases of nasal sinus squamous cell carcinoma were collected in the Department of Otolaryngology of our hospital in July 2009 -2010 years. All cases were not treated with radiotherapy, chemotherapy and other related treatments before operation. The clinical records were complete, After the operation, the pathological examination was performed before the operation.
2. immunohistochemical SP method was used to analyze the positive expression of FSCN and HGF in 3 kinds of rhinitis tissues.
3. using SPSS13.0 statistical package for data analysis, X2 test and Spearman rank correlation analysis. P0.05 was statistically significant.
Result
The positive expression rate of 1.FSCN1 protein was 8.3% (1/12) in the inferior turbinate tissue, and the positive expression rate in the nasal inverted papilloma was 52.5% (21/40). The positive expression rate in nasal sinus squamous cell carcinoma was 78.6% (11/14), and the difference of the three kinds of nasal tissues was statistically significant (P0.05).
The positive expression rate of 2.HGF was 8.3% (1/12) in the inferior turbinate tissue and 40% (16/40) in the nasal inverted papilloma tissue. The positive expression rate in nasal sinus squamous cell carcinoma was 82.9% (12 /14), and the three kinds of nasal tissue expression differences were statistically significant (P0.051).
3.FSCN1/HGF expression was positively correlated with sinonasal papilloma tissue and sinonasal squamous cell carcinoma (P0.05).
conclusion
1. FSCN1 and HGF were highly expressed in nasal inverted papilloma tissues, suggesting that FSCN1 and HGF play an important role in the occurrence and development of intranasal inverted papilloma.
2. FSCN1 and HGF were involved in the process of nasal inverted papilloma, but they were not related to the duration, age and sex of nasal inverted papilloma.
3. the expression of FSCN1 and HGF in nasal inverted papilloma is correlated, suggesting that the two may participate in the process of intranasal papilloma formation.
The expression of 4. FSCN1 and HGF in the inferior turbinate tissue, nasal inverted papilloma and nasal sinus squamous cell carcinoma increased in turn, suggesting that the two may play a role in the transformation of three kinds of nasal diseases.
The expression of 5. FSCN1 and HGF can reflect the objective index of biological behavior of nasal sinus tumor. The over expression of the two may be an early event of squamous carcinoma of inverted papilloma of the nose. By detecting the expression of two cases in three nasal diseases, a new way of prevention and treatment for the prevention and treatment of benign and malignant tumors of the nasal sinuses is provided.
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.62

【参考文献】