p-STAT3、VEGF、MMP-2在中耳胆脂瘤中的表达及意义

发布时间：2018-06-03 13:29

本文选题：中耳胆脂瘤 + 磷酸化信号转导与转录激活因子-3　；参考：《郑州大学》2010年硕士论文

【摘要】： 目的检测磷酸化信号转导与转录激活因子-3(p-STAT3)、血管内皮生长因子(VEGF)和基质金属蛋白酶-2(MMP-2)在中耳胆脂瘤上皮中的表达情况,探讨三者在胆脂瘤上皮增殖与骨质破坏中的作用及其相关性。方法本研究通过收集39例伴有胆脂瘤的中耳乳突炎患者手术中的新鲜胆脂瘤标本,迅速固定备用；术中观察骨质破坏程度,按骨质的破坏程度分为四组：Ⅰ度：7例,Ⅱ度：12例,Ⅲ度：15例,Ⅳ度：5例。同期,取12例正常外耳道皮肤作为对照组。采用免疫组织化学技术(SP法)检测p-STAT3、VEGF及MMP-2在39例中耳胆脂瘤上皮和12例正常外耳道皮肤中的表达,并应用计算机图像分析系统对p-STAT3、VEGF和MMP-2染色结果进行定量分析；分析中耳胆脂瘤组织中p-STAT3、VEGF和MMP-2三者之间的关系。结果 1. p-STAT3蛋白在胆脂瘤组织中阳性表达于上皮细胞胞核中,在血管内皮细胞亦有少量表达,表现为棕黄色颗粒。而在正常外耳道皮肤中p-STAT3蛋白几乎无表达。p-STAT3蛋白在中耳胆脂瘤组织中的表达量为130.80±18.90,在正常外耳道皮肤中的表达量为76.59+13.72,二者之间有显著差异(t=10.917,p0.05)。 2. VEGF蛋白在中耳胆脂瘤组织中的基底部角质细胞和超过基底部的胆脂瘤角质细胞中可有强阳性表达,其阳性表达物位于细胞胞浆,细胞核内无染色,在内皮细胞与周围的炎性上皮下结缔组织的基质细胞中可发现表达强度增加,而在胆脂瘤基质周围组织中可有弱阳性表达。在正常外耳道皮肤基底细胞膜上层可见弱阳性表达。VEGF蛋白在中耳胆脂瘤组织中的表达水平为126.97±13.37,其在正常外耳道皮肤中的表达水平为94.80±18.05,二者之间差异有统计学意义(t=6.698,p0.05)。 3.MMP-2蛋白阳性表达物位于胞浆,细胞核无染色。阳性免疫活性沉积物主要分布于胆脂瘤上皮的颗粒层,棘细胞层与基底层,在上皮下基质中的炎性细胞中可有散在的阳性表达,自颗粒层向内阳性表达程度逐渐增强；在外耳道皮肤的基底层和棘细胞层中可见浅黄色免疫活性沉积物,为弱阳性表达。MMP-2蛋白在中耳胆脂瘤组织中的表达水平为124.46±12.74,而其在正常外耳道皮肤中的表达水平为92.66±14.13,两者之间差异有统计学意义(t=7.373,p0.05)。 4. p-STAT3、VEGF、MMP-2蛋白在中耳胆脂瘤组织中随着骨质的破坏程度增高,其表达量也随之增高,各组之间平均光密度值两两比较,差异有统计学意义(p0.05)。 5. p-STAT3与VEGF蛋白在中耳胆脂瘤中的阳性表达通过相关分析发现二者之间呈正相关(r=0.445,p0.05),p-STAT3与MMP-2蛋白在中耳胆脂瘤中的阳性表达通过相关分析发现二者之间呈正相关(r=0.351,p0.05),VEGF与MMP-2蛋白在中耳胆脂瘤中的阳性表达通过相关分析发现二者之间呈正相关(r=0.568,p0.05)。结论 1.p-STAT3、VEGF及MMP-2蛋白在中耳胆脂瘤上皮中的高表达,提示三者可能在胆脂瘤上皮的增殖过程中发挥重要作用。 2.p-STAT3、VEGF及MMP-2蛋白在中耳胆脂瘤组织中随着骨质破坏程度的范围扩大,其表达量也随之增高,表明其三者在胆脂瘤上皮对骨质的侵蚀行为中起重要作用。 3.p-STAT3与VEGF、p-STAT3与MMP-2及VEGF与MMP-2之间在中耳胆脂瘤组织中的阳性表达呈正向相关,提示三者之间在胆脂瘤组织中的增殖和侵蚀行为中可有正向协同作用,且p-STAT3可能有正向调控VEGF和MMP-2蛋白表达的作用。
[Abstract]:Purpose

To investigate the expression of phosphorylation signal transduction and transcription activator - 3 ( p - 3 ) , vascular endothelial growth factor ( VEGF ) and matrix metalloproteinase - 2 ( MMP - 2 ) in the epithelium of middle ear cholesterin , and to explore the role and correlation of these three factors in the proliferation and destruction of tumor epithelial cells .

method

In this study , we collected 39 cases of fresh cholesterin in the operation of middle ear mastoiditis with cholesterin , and then fixed them rapidly .
In the same period , 12 cases of normal external auditory canal skin were measured by immunohistochemical technique ( SP method ) .
To analyze the relationship between the expression of p - STAT , VEGF and MMP - 2 in the middle ear cholesterin tissue .

Results

1 . The positive expression of p - STAT protein in the cell nucleus of epithelial cells showed that there was little expression in the vascular endothelial cells . The expression of p - STAT protein in the normal external auditory canal was 130 . 80 卤 18 . 90 , and the expression of p - 3 protein in the normal external auditory canal was 76.59 + 13.72 , with a significant difference between them ( t = 10.917 , p0.01 ) .

2 . The expression level of VEGF protein in the basal cell membrane of the normal external auditory canal was 126.97 卤 13.37 . The expression level of VEGF protein in the normal external auditory canal skin was 126.97 卤 13.37 , the difference was statistically significant ( t = 6.698 , p0.05 ) .

3 . The positive expression of MMP - 2 protein was found in the cytoplasm and nucleus . The positive immunoactive deposit was mainly distributed in the granular layer , the spinous cell layer and the basal layer of the biliary epithelioma , and the positive expression was scattered in the inflammatory cells in the subcutaneous matrix , and the expression level of MMP - 2 was gradually increased from the granular layer to the inner positive expression level .
The expression level of MMP - 2 protein in normal external auditory canal skin was 124.46 卤 12.74 and 92.66 卤 14.13 in normal external auditory canal skin ( t = 7.373 , p0.05 ) .

4 . The expression of p - STAT , VEGF and MMP - 2 increased with the degree of destruction of bone in middle ear cholesterin tissues , and the expression level was increased , and the difference was statistically significant ( p < 0.05 ) .

5 . Positive correlation was found between the positive expression of p - STAT and MMP - 2 in the middle ear cholesterin ( r = 0.351 , p0.05 ) , and the positive correlation between VEGF and MMP - 2 was positively correlated ( r = 0.568 , p0.05 ) .

Conclusion

1 . The high expression of p - STAT , VEGF and MMP - 2 in the epithelium of the middle ear cholesterin , suggests that the three may play an important role in the proliferation of the epithelial tumor .

2 . The expression of p - STAT , VEGF and MMP - 2 increased with the extent of bone destruction in the middle ear cholesterin tissue , and the expression level of the three was increased with the extent of bone destruction , indicating that the three of them play an important role in the anti - erosion behavior of the bone .

3 . The positive correlation between the positive expression of p - STAT and VEGF , p - STAT and MMP - 2 and MMP - 2 and MMP - 2 in the tissue of the middle ear was positively correlated , suggesting that there was a positive co - action between the three groups in proliferation and erosion behavior in the tumor tissue , and the expression of VEGF and MMP - 2 may be positively regulated by p - STAT .
【学位授予单位】：郑州大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R764

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